Counting with Droplet Digital™ PCR – More Applications than Anyone Ever Dreamed Of



February 22, 2017

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  • "For more info, visit More than 200 peer-reviewed publications have been published that leveraged the high performance, high throughput, and tremendous versatility of Bio-Rad’s QX100™/QX200™ Droplet Digital PCR (ddPCR™) Systems. The superior precision, sensitivity, and reproducibility that have made these systems the industry standard for digital PCR have produced new biological insights and advanced the use of digital PCR in many new areas of investigation. Among the applications reviewed in this webinar are the adoption of ddPCR for liquid biopsy in cancer therapy monitoring, assisting genome editing of stem cells for disease modeling and therapy, in vivo count monitoring of therapeutically engineered immune cells in HIV eradication strategies, and identification of under-recognized somatic mosaicisms. Droplet Digital PCR Technology is the most precise and sensitive digital PCR solution for a wide variety of applications, featuring flexible assay chemistry optimized for both hydrolysis probe and dye-based assays. Nanoliter droplet partitioning reduces bias from amplification efficiency differences and PCR inhibitors and enables exquisitely accurate target quantification in a convenient assay design, without the use of standard curves. The QX200™ Droplet Digital PCR System, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications. Presenter: Dr. George Karlin-Neumann, Director of Scientific Affairs, Bio-Rad Digital Biology Center Dr. Karlin-Neumann received his PhD in molecular genetics in 1990 from UCLA. After postdoctoral studies with Dr. Ronald Davis in Stanford's Biochemistry Department, he left in 2001 to cofound ParAllele BioScience, which was acquired by Affymetrix in 2005. Dr. Karlin-Neumann joined QuantaLife in 2010. He has since transitioned to his current role as Director of Scientific Affairs at the Digital Biology Center at Bio-Rad Laboratories, where he is responsible for establishing and over-seeing research collaborations exploring new directions for digital PCR technology."

    Analytical Techniques

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