Droplet Digital™ Applications: Phasing of Genetic Variants for CFTR Haplotyping



February 22, 2017

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  • "For more info, visit http://www.bio-rad.com/yt/17/QX200-DropletDigitalPCR In this webinar, Dr. Jack Regan describes how the Droplet Digital PCR (ddPCR™) System can be used to determine whether genomic sequences are physically linked, enabling us to determine disease carrier status, understand differences in allele-specific expression, and clarify genome architecture. Droplet Digital PCR Technology is the most precise and sensitive digital PCR solution for a wide variety of applications, featuring flexible assay chemistry optimized for both hydrolysis probe and dye-based assays. Nanoliter droplet partitioning reduces bias from amplification efficiency differences and PCR inhibitors and enables exquisitely accurate target quantification in a convenient assay design, without the use of standard curves. The QX200™ Droplet Digital PCR System, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications. Presenter: Jack Regan, PhD Group Leader, New Application Development, Bio-Rad Digital Biology Center Dr. Jack Regan helped launch Droplet Digital PCR (ddPCR) as part of QuantaLife. For Bio-Rad Laboratories, he leads a team of scientists that focus on new application development. Prior to working on digital biology, Jack worked on automated platforms, including a sample preparation platform, a sample-to-answer platform for infectious disease detection, and an autonomous platform for the detection of bio-threat agents. The later two platforms were part of his post-doctoral work at Lawrence Livermore National Laboratory. Jack performed his doctoral training at UCSF, where he studied the influenza polymerase. http://www.bio-rad.com/en-us/category/digital-pcr?WT.mc_id=wb-DBC-ww-qx200ddpcr_vyt_20150305_e5cl0HEUY_c"

    Analytical Techniques

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