Precision Molecular Profiling of Cancer Using Droplet Digital™ PCR



February 22, 2017

"For more info, visit Droplet Digital PCR (ddPCR™) technology is being used to identify cancer subtypes, optimize drug treatment plans, monitor residual disease, and study tumor evolution. The recently released Automated Droplet Generator enables minimal hands-on time, high throughput and consistent droplets from any user. Bio-Rad also has assays that target top mutations and other cancer targets readily available for use, no optimization required. Dr. Dawne Shelton discusses how these ddPCR tools will enable your research endeavors. Droplet Digital PCR Technology is the most precise and sensitive digital PCR solution for a wide variety of applications, featuring flexible assay chemistry optimized for both hydrolysis probe and dye-based assays. Nanoliter droplet partitioning reduces bias from amplification efficiency differences and PCR inhibitors and enables exquisitely accurate target quantification in a convenient assay design, without the use of standard curves. The QX200™ Droplet Digital PCR system, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications. Presenter: Dawne Shelton, Ph.D. Senior Scientist, Bio-Rad Digital Biology Center Dr. Shelton received her Bachelor of Science degree in Biochemistry and Molecular Biology from the University of California, Santa Cruz. After 5 years working in the biotech industry at Geron Corporation, she pursued a PhD in Oncological Sciences from the University of Utah, Huntsman Cancer Institute investigating different aspects of the Wnt pathway in colon cancer. Dr. Shelton has studied the origins of the different subtypes of breast cancer by creating mouse models within the context of each cellular lineage. At the University of Iowa she examined uterine cancer in a clinical cancer research lab. She is now a Senior Scientist at the Digital Biology Center focusing on developing applications and collaborations for ddPCR in the field of cancer."

Analytical Techniques

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