Droplet Digital™ PCR Applications for Next-Generation Sequencing



February 21, 2017

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  • "For more info, visit http://www.bio-rad.com/yt/7/QX200-DropletDigitalPCR. Next-generation sequencing (NGS) platforms are currently being used to quantify rare species targets in samples. Heavy processing of the starting template in the NGS experimental workflow can result in biases in the representation of these targets. In webinar, we discuss Droplet Digital PCR (ddPCR™) applications for cross-validating NGS data with respect to rare species targets as well as the excellent capability of ddPCR quantification of NGS libraries to optimize sequencing performance. The QX200™ Droplet Digital PCR system, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications. Presenter: Nicholas Heredia, PhD Staff Scientist, Bio-Rad Digital Biology Center (DBC) Dr. Heredia received his PhD in microbiology, immunology, and molecular genetics at the University of California, Los Angeles and performed postdoctoral studies in biophysics at the Lawrence Livermore National Laboratory. At the DBC, he is focusing on digital applications for NGS as well as advanced R&D research. http://www.bio-rad.com/en-us/category/digital-pcr?WT.mc_id=yt-dbc-ww-qx200-20140210-S_e3rBE5qCA"

    Analytical Techniques

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