Using Droplet Digital™ PCR to Diagnose Leukemia



February 21, 2017

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  • "For more info, visit Prof. Alec Morley, a pioneer in digital PCR, describes his use of droplet digital PCR (ddPCR™) technology to advance the diagnosis and treatment of leukemia. Morley and his colleagues at Flinders University and Medical Center in Adelaide, South Australia developed a new method for the early detection of residual acute lymphoblastic leukemia (ALL) using a DNA marker instead of traditional microscopy of bone marrow cells. The unparalleled sensitivity of the ddPCR system enabled more accurate prediction of relapse risk for ALL patients following chemotherapy. Monoquant, a company founded by Morley to commercialize the research in his lab, is developing a new clinical test for chronic myeloid leukemia (CML). The new method offers higher sensitivity than conventional qPCR tests and also eliminates the variability in amplification efficiency that results from using patient-specific PCR primers, removing a potential roadblock to FDA approval. The QX200™ Droplet Digital PCR system, Bio-Rad’s second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications."

    Analytical Techniques

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