PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells - A Fast Approach to Staining Nuclei

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February 21, 2017

"For more information, visit http://www.bio-rad.com/yt/15/TechSupport-Hoechst This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for fluorescence microscopy and cell imaging applications. Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format. PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution — no weighing required. Features and Benefits of PureBlu Nuclear Staining Dyes • High purity formulation for quality results in challenging experiments • Pre-aliquoted powder format eliminates time-consuming weighing steps • Convenient format allows you to generate working solution with only one dilution step after resuspension • Compatible with multicolor experiments PureBlu Nuclear Staining Dye One-Step Dilution Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to 50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM); PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)). http://www.bio-rad.com/en-us/product/nuclear-staining-dyes?WT.mc_id=yt-gxd-ww-purblu-20141118-BYmvWnwSqdw"

Cell Science

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