Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: Device Failed to Pressurize



February 21, 2017

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  • "For more information, visit This short video describes how to proceed when the Bio-Plex system returns the error message ""Device Failed to Pressurize"", typically due to a mismatch in system pressure detected during calibration, which can occur after replacing the sheath fluid container. The Bio-Plex system uses magnetic beads coupled with unique fluorpohores and customized detection antibodies for the quantitation of multiple proteins, peptides, and nucleic acids. Bio-Rad offers Bio-Plex assays for cytokines, growth factors, signal-transduction molecules, and disease biomarkers for inflammation, autoimmune disorders, cancer, diabetes and heart disease."


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