B16 Mouse Melanoma cancer cells and Dictyostelium amoeba co-culture

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January 16, 2017

Mouse skin melanoma cancer cells (B16, p35) were incubated overnight with dictyostelium amoebae cells (WT1, p14), in order to visualize their interactions. Our digital staining panel allows the discrimination of mammalian cells (blue) and amoeba cells (green) based on their specific refractive index (RI) range. The RI difference (ΔRI 0.015) allows for an automatic segmentation of cell populations. We observed a simultaneous, massive mammalian cell necrosis, the reason of which remains unknown. nanolive.ch/applications/

Imaging/Microscopy

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