Fibroblastic Reticular Cell (FRC) Mitosis

Fibroblastic Reticular Cells (FRCs) were grown to 60% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 45 minutes, capturing images every 30 seconds.