The Cdc16 heat sensitive mutant strain was grown in Yeast Extract medium in 35mm glass bottom culture dish at 36°C and 5% CO2 for 8 hours.
The 3D Cell Explorer took an image every 2 minutes and 30 seconds.
Cdc16 protein has a regulatory function in inhibiting septation during interphase.
In this mutant strain this protein cannot fold properly at 36°C leading to an accumulation of septa during interphase.
Nanolive digital staining allows you to discriminate the different yeast biological components based on their specific Refractive Index (RI) range:
Membrane in Purple, Citosol in Light Blue, Nuclei and Organelles in Yellow, Contractile Ring in Red.