Fission yeast Cdc16 heat sensitive mutant



December 21, 2016

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  • The Cdc16 heat sensitive mutant strain was grown in Yeast Extract medium in 35mm glass bottom culture dish at 36°C and 5% CO2 for 8 hours.
    The 3D Cell Explorer took an image every 2 minutes and 30 seconds.
    Cdc16 protein has a regulatory function in inhibiting septation during interphase.
    In this mutant strain this protein cannot fold properly at 36°C leading to an accumulation of septa during interphase.
    Nanolive digital staining allows you to discriminate the different yeast biological components based on their specific Refractive Index (RI) range:
    Membrane in Purple, Citosol in Light Blue, Nuclei and Organelles in Yellow, Contractile Ring in Red.


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