"Investigation of freshly purified rat tritosomes and human hepatic lysosomes as an in vitro tool for characterization of biologic drugs"
Presented by Chris Bohl, PhD, Global Technical Support Manager for Sekisui XenoTech’s Products Division, on Feb. 4th, 2016.
Synopsis: The realization of new technologies and the development of targeted biopharmaceutical therapies have accelerated in the last decade with over 30 such compounds currently in clinical trials. With ongoing development and evaluation of strategies, such as antibody drug conjugates, to effectively deliver compounds to targeted cell populations through the endocytic-lysosomal pathway, the development and availability of novel reagents will become paramount. Isolated rat hepatic tritosomes and human hepatic lysosomes are in vitro systems that can be utilized to quickly and conveniently evaluate compound stability and guide development direction of biopharmaceutical candidates. In this study, subcellular isolation techniques combined with immunoblotting, protease arrays, and enzymatic activity assays were carried out to characterize isolated rat tritosomes and human hepatic lysosomes.
Key Concepts Discussed:
Isolation techniques and characterization
Insights into the differences between tritosomes and lysosomes
In vitro applications
Download Slides: https://www.xenotech.com/webinar-slides/xenotech_lysosomes_webinar_slides.pdf
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