Western Blotting - the principles and a comparison of indirect vs. direct immunodetection

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Expedeon

Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. Although Western blotting is simple in principle, for a successful outcome, scientists must be aware of the technical caveats which must be overcome. Therefore, to provide further insights in to Western blotting, this exciting webinar discusses: • Sample preparation • SDS-PAGE • Western Blot transfer • Blocking • Indirect Detection -- primary antibody incubation and indirect detection • Indirect Vs. Direct antibody detection -- advantages and disadvantages • Direct detection for multiplex fluorescent Western blotting • Problems with sourcing labeled antibodies for direct detection • The solution simple antibody labeling kits • Questioning the secondary antibody amplification hypothesis