Behind the Science, ep. 8: "Bottoms" Up

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Waters Corporation

Protein quantification: bottoms up or bottom-up? As our host Jen Fournier learns, LC-MS/MS is a good alternative to ligand binding assays, and Waters scientists have been developing methods and technologies to support protein quantification for more than 8 years. Erin Chambers, principal scientist, shows Jen how to perform a surrogate peptide approach, where a protein is digested into its peptide, and a signature peptide is selected to monitor as a representative in a quantitative assay. No beer mugs in the lab!