Real-time Monitoring of Phagocytosis Using the IncuCyte® ZOOM Instrument

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Essen Bioscience

Real-time monitoring of phagocytosis using the IncuCyte® ZOOM live-cell imaging & analysis system. Time-lapse phase contrast and fluorescence images, acquired using IncuCyte® ZOOM instrument, enable the real-time visualisation of IncuCyte™ pHrodo® Green E. coli Bioparticles® engulfment by the mouse macrophage cell type J774A.1. The Bioparticles® are phagocytosed and enter the acidic environment of the phagosome resulting in increased fluorescence of the pHrodo® label. IncuCyte® ZOOM integrated image analysis tools enable detection and measurement of the green fluorescent signal over the entire assay time-course and eliminate the impact of background fluorescence.