HT1080 Fibrosarcoma Cells Under Control Conditions in an Incucyte™ Cytotoxicity Assay

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July 27, 2016

Time-lapse movie of HT1080 fibrosarcoma cells under control conditions in an IncuCyte™ Cytotoxicity Assay. In this assay cell death is detected in real time using the mix-and-read IncuCyte™ Cytotox Green Reagent. When added to growth medium, the IncuCyte™ Cytotox Reagent is inert, non-fluorescent and does not enter viable cells. As cells die and membrane integrity is lost, the IncuCyte™ Cytotox probe enters the cell and fluorescently labels the nuclei. Dying cells are identified and quantified over time using the IncuCyte ZOOM® system to detect the appearance of green labelled nuclei. High definition phase contrast images and movies provide an additional qualitative validation of cell death based on morphology (e.g. loss of cytoskeleton structure, loss of motility).

Cell ScienceDrug DiscoveryImaging/Microscopy

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