Leukocyte extravasation. HUVECs were seeded at 6,000 cells per ClearView insert well and allowed to form a monolayer for ~24 hrs. CD3/CD28 activated primary T-cells (5000 cells/insert well) were placed on top of HUVEC monolayer prior to exposing the cells to an SDF-1α gradient. Images were collected every minute using the IncuCyte™ ZOOM imaging system at 10x.
Cell ScienceDrug DiscoveryImaging/Microscopy