Chemotaxis Transendothelial Migration with IncuCyte™

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Essen Bioscience

Leukocyte extravasation. HUVECs were seeded at 6,000 cells per ClearView insert well and allowed to form a monolayer for ~24 hrs. CD3/CD28 activated primary T-cells (5000 cells/insert well) were placed on top of HUVEC monolayer prior to exposing the cells to an SDF-1α gradient. Images were collected every minute using the IncuCyte™ ZOOM imaging system at 10x.