T cell killing SK OV 3 Ovarian Cancer Cells in real time



July 27, 2016

  • Share
  • An IncuCyte™ movie showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. The IncuCyte™ Caspase 3/7 apoptosis reagent enables detection of cells undergoing apoptosis in real-time (green fluorescence). Labeling of the SK-OV-3 cancer cells with the NucLight Red™ Live-Cell Labeling reagent enables simultaneous measurement of tumor cell proliferation. IncuCyte™ Immune Cell Killing Assay concept 1. Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs). 2. Tumor cell death is measured directly and in real-time by adding the mix and read IncuCyte™ Caspase 3/7 Reagent to your cultures. 3. Automated image analysis enables selective quantitation of tumor cell death. Optional - simultaneous tumor cell counts can be enabled by labeling target cells with our range of NucLight™ Live-Cell Labeling Reagents.

    Cancer Research

    Keep up to date with all your favourite videos and channels.

    Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.