T cell killing SK OV 3 Ovarian Cancer Cells in real time

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Essen Bioscience

An IncuCyte™ movie showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. The IncuCyte™ Caspase 3/7 apoptosis reagent enables detection of cells undergoing apoptosis in real-time (green fluorescence). Labeling of the SK-OV-3 cancer cells with the NucLight Red™ Live-Cell Labeling reagent enables simultaneous measurement of tumor cell proliferation. IncuCyte™ Immune Cell Killing Assay concept 1. Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs). 2. Tumor cell death is measured directly and in real-time by adding the mix and read IncuCyte™ Caspase 3/7 Reagent to your cultures. 3. Automated image analysis enables selective quantitation of tumor cell death. Optional - simultaneous tumor cell counts can be enabled by labeling target cells with our range of NucLight™ Live-Cell Labeling Reagents.