IncuCyte Poster Snapshots | Quantitative Live-Cell Imaging Assays for Immunotherapy

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Essen Bioscience

Quantitative live-cell imaging assays for immunotherapy: chemotaxis, immune cell killing & phagocytosis G. Lovell, N. Bevan, C. Szybut, N. Rodinova, D. Appledorn, M. Tikhonenko, L. O’Clair, T. O’Callaghan, T. Dale & D. Trezise. Essen BioScience R&D, Welwyn Garden City, UK & Ann Arbor, Michigan, USA Presented at CIMT 2016, Germany Summary & impact: • For the body to defend and fight against cancer, immune cells must recognise, engage, destroy and ultimately remove unwanted tumour cells. Understanding these processes and interactions at the cellular level is central to identifying and validating new drug targets and cellular therapy approaches. • Essen BioScience offers a flexible range of phenotypic assays to explore all aspects of the immuno-oncology research area. All of these assays are based on noninvasive live-cell analysis of cells in 96-well micro-plates using an IncuCyte® ZOOM live cell analysis system. • Here we describe a cluster of new assays for quantifying immune cell biology and interactions with tumour cells. • Phase-contrast/fluorescence images from cells gathered over time are processed to measure phenotypes, such as immune cell proliferation, migration & death. • Experiments are performed with low cell numbers using simple mix and read formats which are non-perturbing to the cell model. • Each of these approaches provide a full time-course of the biology; images and time-lapse movies provide credibility and valuable biological insight. Added throughput and automated image analysis enhances productivity.