Application of Hepatocytes for In Vitro Uptake Assays Webinar

4,141 views

|

February 6, 2013

This webinar provides a basic review of concepts applicable to a structure-based approach for the evaluation of hepatic uptake with in vitro hepatocyte experiments.

Key concepts discussed in this webinar will include:
- Selection of appropriate in vitro study type and design based on predictions from a drug candidate's phys-chem properties
- Description of the basic in vitro methodologies using hepatocytes, both suspension and plated, to evaluate drug uptake, namely whole-system versus medium loss, oil-spin method and uptake into plated cells
- Discussion of the practical challenges associated with these experiments
- Basic review on the estimation of active and passive uptake components from these in vitro hepatocyte experiments
- Impact of phys-chem properties, active and passive uptake and metabolism on the estimation of free intracellular drug concentrations
- Brief review of the prediction of in vivo hepatic clearance from in vitro uptake experiments and potential scaling techniques

Download Slides: https://www.xenotech.com/wp-content/uploads/2013/02/XenoTech-Uptake-experiments-in-hepatocytes.pdf

Questions: https://www.xenotech.com/contact-us

Upcoming Webinars: https://www.xenotech.com/scientific-resources/upcoming-webinars

Other Previously Aired Webinars: https://www.xenotech.com/scientific-resources/webinar-series

Cell CultureChemistryDrug Discovery

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.