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Higher Resolution Improves Top-Down Protein Identification on Orbitrap Mass Spec for Large Proteins

from Chromatography & Mass Spectrometry Solutions

Higher Resolution Improves Top-Down Protein Identification on Orbitrap Mass Spec for Large Proteins David Horn - Product Manager, Biosoftware David Horn presents his scientific poster on the necessity for high mass resolution (more than 200,000) for confidence in identification of proteins above 40kDa using yeast enolase. The comparison between various resolution settings for both direct infusion and LC-MS/MS data are shown, using the Thermo Scientific Orbitrap mass spectrometer to improve confidence and sequence coverage for identification of larger proteins. The high-field Orbitrap analyzer increases speed, sensitivity and dynamic range to dig deeper into your samples. The ultra-high resolution provides certainty in analytical results by enabling molecular weight determination for intact proteins and in-depth analysis of isobaric species.

about 6 years ago

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