Mass Spectrometry

Shimadzu Analytical & Measuring

Extractables and leachables from food packaging have the potential to cause serious health issues. To ensure consumer safety and regulatory compliance, testing labs need to have reliable methods for the detection of these contaminants in food contact materials (FCMs). 

Using a Shimadzu quadrupole time-of-flight (Q-TOF) mass spectrometer, we developed a proven method for fast, accurate identification of extractables and leachables in FCMs.

Watch the video to learn how you can analyze common food storage products, including a plastic bag, a plastic cup and aluminum foil, in a total run time of 15 minutes.


Fast, Reliable Detection of Extractables & Leachables in Food

Sekisui XenoTech

Presented by SEKISUI XenoTech Technical Support Managers, Drs. Andrew G. Taylor and Chris Bohl Metabolic stability influences the oral bioavailability and plasma half-life of a compound. Metabolic stability assays measure intrinsic clearance and determine the extent to which a drug will be metabolized. This study can be conducted in a variety of test systems, which is why it may also be referred to as a hepatocyte stability study, microsomal stability study, etc. This quick overview video discusses: - Test systems for stability assays - Stability study endpoints - Example data in different species - Considerations for those wanting to conduct stability studies Download slides: https://www.xenotech.com/wp-content/uploads/2020/11/SEKISUI-XenoTech_Metabolic-Stability-ADME-101.pdf Questions, comments and requests: https://www.xenotech.com/contact/ More information on our research services and test systems: https://www.xenotech.com Other previously aired webinars: https://www.xenotech.com/access-adme-research-resources/resource-type/webinar/ Upcoming webinar notifications: https://www.xenotech.com/contact/newsletter-sign-up/ Request a webinar topic: https://www.xenotech.com/contact/webinar-seminar-request/ About the Presenters: Dr. Andrew G. Taylor received his Ph.D. from University of California San Diego. He joined SEKISUI XenoTech as a research scientist in 2017, serving as a Study Director in nonclinical drug interaction contract studies in compliance with Good Laboratory Practices (GLP), OECD Principles of GLP, &/or Japan MOHW GLP Standards and specializing in drug transport and drug metabolism studies. He became the Technical Support Manager for services in 2020 and provides valuable guidance to ensure research needs are being met. Dr. Chris Bohl received his PhD from the School of Biological Sciences from the University of Nebraska – Lincoln and has been with SEKISUI XenoTech since 2014, first operating as a research scientist before becoming the Senior Manager of Technical Support for Products. Chris provides valuable guidance to scientists seeking to choose the correct test system, troubleshoot difficulties and much more.

ADME 101: Drug Metabolism Studies – Metabolic Stability

Analytical Cannabis 

With the passing of the 2018 Farm Bill legalizing hemp and different US states legalizing Cannabis products, farmers are allowed to grow cannabis and hemp. Like any other agricultural crop, pesticides are applied to cannabis and hemp plants to protect it from pests and improve growth yield. Chronic exposure to pesticides can lead to serious health risks and therefore pesticide analysis in Cannabis and hemp is important for consumer safety and quality control. Among all of states in USA, California has issued most stringent action limits for 66 pesticides residues in cannabis flower, cannabis infused edibles, concentrates and hemp.  Normally pesticide analysis in cannabis and other food matrices is done by both GCMSMS and LCMSMS since some nonpolar and chlorinated pesticides such as pentachloronitrobenzene, chlordane and others are difficult to ionize with electrospray source used in LCMSMS systems.   We would demonstrate how we measured these compounds at low levels using LCMSMS with APCI source. The ionization mechanism of chlorinated pesticides such as pentachloronitrobenzene and others using LCMSMS system with APCI source would be presented. We analyzed all of 66 pesticides (including very hydrophobic and chlorinated pesticides typically analyzed by GCMSMS) spiked in  hemp and cannabis samples  well below the action limits specified by California State. Hemp and cannabis matrix is challenging and therefore it causes ion suppression and matrix inference for response of some of pesticides. We would demonstrate approaches to mitigate these matrix effects from cannabis and hemp. Sample preparation is the main bottleneck for achieving the high throughput analysis of pesticides in cannabis/hemp testing labs. For sample preparation, we used solvent extraction since it is a quick, cheap and easy way to achieve high extraction recovery in comparison to other time-consuming sample preparation techniques like solid phase extraction (SPE) and QuEChERS. Also, the performance of LC-MS/MS method was checked by conducting blind emerald scientific’s proficiency test for 66 pesticides in hemp. The proficiency test report generated with LCMSMS method showed acceptable results for all of 66 pesticides in hemp with no false positives and negatives.

Overcoming Challenges associated with analysis of all of pesticides regulated by California

Real-time PCR is the method of choice for contemporary microbiological quality analysis of cannabis products. Its reliability is already proven for many years for analysis of food products and beverages, outcompeting traditional culture in both specificity and sensitivity. With the ever increasing amount of cannabis-infused products on the market, BIOTECON Diagnostics as long standing partner in the food safety diagnostic industry can bring all its experience to the market. 
 Matrices in Cannabis and Cannabis-infused products can range from pure plant materials with lots of secondary metabolites to highly processed products, such as gummy bears and brownies. The sheer variety of matrices are challenging to come by, especially in regards to sample preparation and DNA extraction in many labs. The efficient removal of dead cell background, where necessary, and PCR-inhibitors during extraction is what sets BIOTECON’s extraction apart. As a leader in viability PCR, BIOTECON’s Reagent D eliminates dead microbes from the PCR, enabling results that perfectly match traditional culture.
With over 20 years of experience in real-time PCR analysis, BIOTECON Diagnostics has assembled a portfolio of open-platform cannabis testing solutions combining highest sensitivity, specificity, speed and convenience that perfectly fits the needs of any lab. This includes our unique foodproof Yeast and Mold Quantification LyoKit and our brand new multiplex kit for Aspergillus detection and identification. 
With its long standing history and experience in real-time PCR based kits, BIOTECON Diagnostics is a pioneer and a premier partner to support clients in the Cannabis industry to ensure product safety.

qPCR Testing for microbial contaminants in Cannabis and Cannabis-infused products

Speaking at The Science of Cannabis 2020 Online Symposium, Sarah Dalton, US Hemp and Cannabis Leader for Ernst & Young presented her talk on the challenges of regulating CBD from the FDA perspective.

Abstract: Recent warning letters and Consumer Updates from the FDA have sent a chill through the CBD industry. Anticipated market entrants like Coca-Cola, Mondelez, Starbucks and Kellogg’s that would have bought up much of the CBD inventory produced from a record-year of hemp cultivation have mostly stayed on the sidelines, leading to a precipitous fall in hemp and hemp derivative prices. This regulatory uncertainty is leading CBD companies to come up with novel approaches to develop and market their products while staying afloat in this volatile market. Join Sarah as she discusses what the current FDA position on CBD is, projected regulatory rules and pathways, existing CBD products and their ability to market medical claims including OTC monograph topicals, and the role of individual states in regulating CBD, THC and minor cannabinoids. 

The FDA challenge for regulating CBD 

CEM_Corporation

Have you ever wondered how the world around us is scientifically analyzed?  From food to fuel, the key is preparing the sample.  

Microwave sample preparation is a proven technique that has been utilized for many years for a wide variety of sample types for elemental analysis by ICP, ICP-MS, or AA.  Acid digestion is employed to break down the sample matrix leaving the elements of interest in solution and ready for analysis. CEM microwave digestion systems rapidly break down a wide variety of sample matrices leaving behind a clear solution containing the analytes of interest.

CEM helped pioneer the field of microwave-enhanced chemistry and many official methods were developed by chemists using CEM systems. That’s why more chemists trust CEM microwave sample preparation systems than any other system available for microwave digestion. 

In an effort to show how sample preparation is done, we took your suggestions and created "Will It Digest?". Enjoy.

Will it Digest? Big Mac

It's a sticky, gooey mess and difficult to weigh out, never mind digest, but Michael Howe has never met a sample that he didn't try. First peanut butter digestion challenge: get the sample into the bottom of the digestion vessel without making a mess. Second challenge: digest a mixed group of food samples, including peanut butter, beef jerky, a lollypop, and cheese. Third challenge: digest 40 of them at once! Howie is going to try...

Can we reach 210 C with 40 samples, the temperature required for a complete peanut butter digest? Let's find out in this episode of Will it Digest?

Learn more: cem.com/microwave-digestion

Will it Digest? Peanut Butter

For over 30 years chemists have trusted CEM to provide the highest quality microwave digestion systems for their laboratories. The MARS 6™ is simply the easiest to use, safest, and most intelligent system on the market. It is the only system that uses integrated sensor technology to recognize the vessel type as well as the sample number then apply the appropriate amount of power to ensure the highest quality digest. With options for remote system control and contactless all vessel temperature control, the MARS 6 is ready to handle your most difficult samples. Features -Contactless in-situ temperature measurement -Sensors that detect vessel type and count -Hundreds of preprogrammed methods -Easy to use vessels for every application

MARS 6 Demo

Flour, salt, water, and surfactant seem simple to digest, but what happens if we add a rubber ducky, a crayon, and a plastic figure to the same batch? Will they all digest? What kind of sample prep is involved? Howie takes on several different toys in larger sample sizes in this episode of Will it Digest? With all of the mixing and matching that he has done, as always, the question is Will it Digest?

Mass Spectrometry

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