Proteomics and Metabolomics

Cytiva

Sensitive, flexible CCD-based imagers designed to support FDA 21 CFR Part 11 EU GMP Annex 11 regulations

Amersham™ brand ImageQuant™ 800 GxP biomolecular imager – Cytiva

Creative Proteomics videos

Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method based on mass spectrometry that identifies and quantifies relative differential changes in protein abundance. First used in quantitative proteomics in 2002, it provides accurate relative quantification without any chemical derivatization or manipulation.

https://www.creative-proteomics.com/

Stable Isotope Labeling

iTRAQ (Isobaric tag for relative and absolute quantitation) was developed by Applied Biosystems Incorporation in 2004. iTRAQ is a isobaric labeling method to determine the amount of proteins from different sources in just one single experiment. The iTRAQ reagents available always are set of 4-plex and 8-plex mass tages that can be used to label and et quantitative information on up to 4 and 8 different samples in one experiment
https://www.creative-proteomics.com/services/itraq-based-proteomics-analysis.htm

iTRAQ-based Proteomics Approaches

PTMs refer to the covalent and generally enzymatic modification of proteins during or after protein biosynthesis. It denotes changes in the polypeptide chain as a result of adding distinct chemical moieties to amino acid residues. PTMs are the foundation of governing intricate cellular process, such as cell division, growth, differentiation, signaling and regulation. Also, PTMs are involved in many cellular processes including the maintenance of protein structure and integrity, regulation of metabolism & defense processes, cellular recognition and morphology alternation. Consequently, analysis of protein post-translational modifications, including the modification categories and modified sites, is particularly crucial for the study of cell biology and disease diagnostics and prevention.

With years’ experience in advanced experiment equipment, Creative Proteomics can provide a variety of PTM services to assist your scientific research. https://www.creative-proteomics.com/services/protein-post-translational-modification-analysis.htm

Introduction of PTM

Teach Me in 10

This week at Teach Me in 10, we are joined by Oded Rechavi, a professor in molecular biology at Tel Aviv University in Israel. The tagline for the Rechavi lab is "The Laboratory for Radical Science", and in this ten-minute talk, you will learn why. Rechavi's research spans various different topics, including the study of novel mechanisms of inheritance and applying genomics analyses to the ancient Dead Sea Scrolls. To learn more about the Rechavi laboratory, you can visit the website, or read the profile piece Technology Networks published in 2020.

The Laboratory for Radical Science With Professor Oded Rechavi

Acetylation refers to a reaction that introduces an acetyl functional group into a chemical compound, in which the hydrogen atom of a hydroxyl group is replaced by an acetyl group (CH3CO) to yield a specific ester, the acetate. Protein acetylation commonly has two different forms. In humans, almost (80%-90%) proteins become co-translationally acetylated at their Nα-termini of the nascent polypeptide chains. Another type is typically acetylated on lysine residues. More can be found at https://www.creative-proteomics.com/

Brief Introduction of Protein Acetylation

Protein phosphorylation, a reversible process, is characterized by adding phosphate donated from ATP and removing phosphate from a phosphorylated protein substrate. And the protein phosphorylation is catalyzed by protein kinase and phosphatase (PP). In eukaryotic cells, about 86.4% of the protein phosphorylation events occur on serine (Ser or S), while 11.8% protein phosphorylation events occur on threonine(Thr or T) whereas only 1.8% on tyrosine (Tyr or Y) residues.  https://www.creative-proteomics.com/

Phosphorylation analysis by mass spectrometry

Peptide Mass Fingerprinting (PMF), also known as mass fingerprinting, was developed in 1993. It is a high throughput protein identification technique in which the mass of an unknown protein can be determined. PMF is always performed with Matrix-assisted laser/desorption ionization time of flight (MALDI-TOF) mass spectrometry. Learn more at https://www.creative-proteomics.com/resource/the-principle-characteristics-and-application-of-peptide-mass-fingerprinting.htm

Protein identification-Peptide mass fingerprinting (PMF)

Lucid Scientific

REAL-TIME CELL ANALYSIS
The RESIPHER series of cell culture monitors are the world's first handheld devices to measure oxygen consumption in standard multi-well plates, providing continuous insight directly from your incubator.

STREAM ANYWHERE
Monitor and analyze your data from anywhere with Lucid Lab - a cross-platform app for managing experiments, monitoring performance, and visualizing data. With our extensible open API, Lucid supports a variety of custom system integrations.  Gain insights into your experimental results remotely and rapidly iterate your experiment designs with your team in real-time.

TECHNOLOGY
To achieve its capabilities, RESIPHER incorporates multiple innovations. Key among these are an array of ultra-sensitive dissolved oxygen sensors developed by Lucid, along with dynamic vertical micro-movements that scan the oxygen gradient formed by metabolically active cells. These innovations give researchers insights into dynamic cellular responses never before seen.

CONFIGURABLE SYSTEMS
RESIPHER systems are currently available in 4-channel and 32-channel formats. With up to 8 devices on a single hub, the RESIPHER can meet the needs of any lab, big or small.  Sensing lids arrive sterilized and calibrated and are compatible with standard 96-well plates including Nunc, Falcon, and Greiner.

COLLABORATE
Lucid Lab's public platform (pub.lucidsci.com) provides a space for exploring shared experiment data, while the private space (lab.lucidsci.com) is the portal to your experiments both private and public.  At pub.lucidsci.com experiment data showing cell growth and drug dose response are featured. Additional showcase experiments are added continuously as they are performed.

Proteomics and Metabolomics

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