Proteomics and Metabolomics

Creative Proteomics videos

Proteins, biomolecules or macromolecules, perform a wide range of functions in organisms. Almost all of the cellular processes require that proteins specifically recognize a multitude of different interaction partners. They can carry out their roles by interacting with other molecules, including DNA, RNA, proteins and small molecules. Protein and protein interactions (PPIs), which refers to intentional physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces. https://www.creative-proteomics.com/Services/Co-immunoprecipitation-co-IP.htm

Brief Introduction to Protein-Protein Interaction (PPI)

Glycan microarray has become a sensitive and versatile approach for the analysis of glycosylation changes in complex biological samples. It can not only investigate glycan-GBPs binding but also provide kinetic analysis of glycan–protein interactions. Nowadays, this application has rapidly expanded and now achieves successful discrimination of selective interactions between glycans and, not only proteins, but also viruses, bacteria and eukaryotic cells, and most recently even live cell responses to immobilized glycans. Glycan microarray can be used to discover functional glycans, characterize glycan-processing enzymes, and detect pathogens for diagnosis. It is also suitable for monitoring multiple molecular interactions with biomolecules where glycans are involved, offering a large variety of bioassay options.

As one of the leading companies in the omics field with over years of experience in omics study, Creative Proteomics provides Glycan Microarray Assay customized to your needs. https://www.creative-proteomics.com/services/glycan-microarray-assay.htm

Glycan Microarray Assay

Proteins are the critical players in molecular recognition at the core of all biological processes. They can interact with the other components of cells, such as small-molecule metabolites, nucleic acids, membranes, and other proteins to build supramolecular assemblies and elaborate molecular machines that perform all sorts of functions, from chemical catalysis and mechanical work to signaling and regulation. As one of the most important interactions, Protein-Protein Interactions have been studied widely. So far large scale protein-protein interactions have been identified, and all the generated data collected together in specialized databases, enables the creation of large protein interaction networks. https://www.creative-proteomics.com/services/protein-protein-interactions-analysis-service.htm

Techniques for Protein-Protein Interaction

MS is widely used in free oligosaccharides, glycosaminoglycans as well as the glycan portions of glycoproteins, proteoglycans, and glycolipids. And there is a wide range of MS techniques are available for glycomics analysis. As research continues on MS-based glycomics analysis, we believe that clear understanding the mechanisms of important biological processes will be achieved in the near future. https://www.creative-proteomics.com/

Glycomics Analysis by Mass Spectrometry

Aging (Aging-US)

Aging-US published this research paper on July 12, 2022 in Volume 14, Issue 13, entitled, "Age-related neuroendocrine, cognitive, and behavioral co-morbidities are promoted by HIV-1 Tat expression in male mice" by researchers from Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS; Department of Psychiatry, McLean Imaging Center, McLean Hospital/Harvard Medical School, Belmont, MA; Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS.

DOI - https://doi.org/10.18632/aging.204166

Corresponding author - Jason J. Paris - parisj@olemiss.edu

Abstract

In the U.S. about half of the HIV-infected individuals are aged 50 and older. In men living with HIV, secondary hypogonadism is common and occurs earlier than in seronegative men, and its prevalence increases with age. While the mechanisms(s) are unknown, the HIV-1 trans-activator of transcription (Tat) protein disrupts neuroendocrine function in mice partly by dysregulating mitochondria and neurosteroidogenesis. We hypothesized that conditional Tat expression in middle-aged male transgenic mice [Tat(+)] would promote age-related comorbidities compared to age-matched controls [Tat(−)]. We expected Tat to alter steroid hormone milieu consistent with behavioral deficits. Middle-aged Tat(+) mice had lower circulating testosterone and progesterone than age-matched controls and greater circulating corticosterone and central allopregnanolone than other groups. Young Tat(+) mice had greater circulating progesterone and estradiol-to-testosterone ratios. Older age or Tat exposure increased anxiety-like behavior (open field; elevated plus-maze), increased cognitive errors (radial arm water maze), and reduced grip strength. Young Tat(+), or middle-aged Tat(−), males had higher mechanical nociceptive thresholds than age-matched counterparts. Steroid levels correlated with behaviors. Thus, Tat may contribute to HIV-accelerated aging.

Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.204166

Keywords - aging, hypothalamic-pituitary-adrenal axis, hypothalamic-pituitary-gonadal axis, secondary hypogonadism, trans-activating transcriptor

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

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LinkedIn - https://www.linkedin.com/company/aging/
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Aging-US is published by Impact Journals, LLC: http://www.ImpactJournals.com​​

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Trending With Impact: Age-related Co-morbidities Promoted by HIV-1 Tat Expression in Male Mice

SCIEX2

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Proteomics is a large-scale comprehensive study of a specific proteome, including information on protein abundances, the variations and modifications, as well as their interacting partners and networks. In discovery proteomics, proteome analysis can be performed in two different strategies, bottom-up and top-down approaches, respectively. In the bottom-up approach, a crude protein mixture undergoes protease digestion first, and then separation by liquid chromatography, followed by MS analysis. In the top-down method, proteins are characterized by MS without prior proteolysis. This type of approach has the advantage of providing greater sequence coverage, resolution of sequence ambiguities and preservation of PTMs. https://www.creative-proteomics.com/services/proteomics-service.htm

Protein Sequencing

Glycomics, the study of glycans, is applied to biology and chemistry that focuses on the structure and function of carbohydrates, and on glycoform distributions at the cellular, tissue, organ and organism levels. In glycomics, there are three major parts, including structural characterization of glycans, research of glycan–protein interactions, and the study of in vitro and in vivo systems for the determination of the function of specific glycans. https://www.creative-proteomics.com/services/glycomics-service.htm

Glycomics analysis by using mass spectrometry

Creative Proteomics has established a highly sensitive HPLC-MS/MS pipeline that can analyze N- and O-methylation in both eukaryotic and prokaryotic organisms. In addition, we have optimized our protocol, to enable more fast and sensitive site mapping service for methylation analysis.

https://www.creative-proteomics.com/services/Methylation.htm

Proteomics and Metabolomics

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