Proteomics and Metabolomics

Teach Me in 10

What can integrative structural biology bring to viral research? In the last year we have seen viral research hit the spotlight as the world has battled against the SARS-CoV-2 virus. It has emphasized how critically important it is for us to be able to study what viruses look like, how they are structured and how they behave in order to develop diagnostic tests, therapeutics and preventives. Integrative structural biology methods are really key here, as explained by our Teach Me in 10 guest, Dr Albert Konijnenberg.

Structural Biology Goes Viral With Dr Albert Konijnenberg

Arrayjet

Arrayjet manufacture the most advanced inkjet liquid handling for multiplex array-based diagnostics and POC devices.

An expert in bioprinting, Arrayjet install microarrayers and offer custom array printing services, worldwide.  The non-contact technology is up to 100-fold faster than pin-spotting or tip-dispensing systems and the Ultra Marathon series can print up to 1000 slides (or 200 microplates) in a single batch for commercial manufacture.

An optical QC system, the Iris, involves twin cameras that sit either side of the print head and work with intuitive software to evaluate printing in real-time.  Any missing spots are detected and reprinted automatically, and a full QC report is available at the end of each print run.

Arrayjet: Inkjet microarray printers in action

Creative Proteomics videos

SILAC-based quantitative proteomics (SILAQ) is an innovative technology used in high throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions. https://www.creative-proteomics.com/Services/SILAC-based-proteomics-analysis.htm

SILAQ

Creative Proteomics has established a highly sensitive HPLC-MS/MS pipeline that can analyze N- and O-methylation in both eukaryotic and prokaryotic organisms. In addition, we have optimized our protocol, to enable more fast and sensitive site mapping service for methylation analysis.

https://www.creative-proteomics.com/services/Methylation.htm

Protein Methylation Analysis

Protein-protein interactions (PPIs) are the basis of the cellular structure and functions. It has been estimated that more than 80% of proteins do not operate alone but in complexes. The analysis of PPIs is able to help us understand cellular organization, process, and functions. PPIs analysis may discovery unique and unforeseen functional roles for well-known proteins. And it can discover previously unknown proteins by association knowns proteins. https://www.creative-proteomics.com/

PPI Methods

Edman degradation, which was developed by Pehr Edman, is a method to sequence amino acids in a peptide. It is the Cyclic degradation of peptides based on the reaction of phenylisothiocyanate with the free amino group of the N-terminal residue.  More at https://www.creative-proteomics.com/

Protein Sequencing – Edman Degradation

At Creative Proteomics, we can provide de novo peptides or protein service. Equipped with state-of-the-art technologies such as high-performance liquid chromatograph coupled to tandem mass spectrometry (LC-MS/MS) and advanced computational algorithms, we can offer accurate and fast de novo peptide/protein sequencing service customized to your needs.
https://www.creative-proteomics.com/

Introduction to Novo peptide sequencing

Creative Enzymes

In biochemistry, the substrate is a molecule upon which an enzyme acts. Enzymes catalyze chemical reactions involving the substrate(s). In the case of a single substrate, the substrate bonds with the enzyme active site, and an enzyme-substrate complex is formed. The substrate is transformed into one or more products, which are then released from the active site. The active site is then free to accept another substrate molecule. In the case of more than one substrate, these may bind in a particular order to the active site, before reacting together to produce products. https://www.creative-enzymes.com/service/Screening-Of-Substrates-Inhibitors-And-Other-Ligands_65.html

Screening of Substrates, Inhibitors, and Other Ligands

Shotgun proteomics refers to the use of bottom-up proteomics techniques to study the whole proteins in a complex mixture, such as serum, urine, and cell lysates, etc. It utilizes the technology of high performance liquid chromatography (HPLC) in combination with mass spectrometry (MS) technology. The most distinctive feature of shotgun proteomics is that it enables identification and comparative quantification of a wide range of proteins at the same time with minimal protein separation needed.  More information can be found at https://www.creative-proteomics.com/services/shotgun-protein-identification.htm

Proteomics and Metabolomics

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