Proteomics and Metabolomics

Octet® Bio-Layer Interferometry (BLI) Platform

Label-Free High-Quality Kinetics and Quantitation in Real-Time - Octet®️ Bio-Layer Interferometry (BLI) systems offer an advanced, fast, robust, and fluidics-free approach for protein-protein and protein-small molecule analysis. Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection
The Octet®️ platform is comprised of instruments, software, biosensors, reagents and assay kits for biomolecular interactions analysis in standard microplates. All Octet®️ instruments have comparable sensitivity and direct detection of specific proteins or drug molecules - even in complex mixtures and unpurified samples such as cell culture supernatants and lysates. The Octet®️ platform is a scalable solution that can process up to 96 samples in parallel. This unprecedented throughput and flexibility will empower scientists by enabling faster time to market, quicker project completion, and greater project capacity.

00:02 The Sartorius Octet® allows researchers to analyze protein-protein and protein-small molecule interactions and represents the fluidic free industry standard for label-free analysis.
00:14 The Bio-Layer Interferometry platform includes instruments biosensors reagents assay kits and software to measure binding affinities kinetics and concentrations in real time.
00:27 This integrated solution makes it significantly faster and easier to perform robust characterization of biomolecules and drug candidates accelerating workflows and delivering stress-free data for early decision making.
00:42 Sartorius's latest offering the Octet® R series provides a modular and scalable solution for molecular screening in 96 well microplates.
00:51 Offering a choice of two four or eight channel parallel processing, these instruments are designed to provide fully automated walk-away processing to meet every lab's budget and throughput requirements.
01:06 The entry-level octet r2 is intended for labs with low throughput requirements or a limited budget.
01:12 It brings the benefits of bio-layer interferometry to a wider audience, including features such as robust temperature control and microplate cooling that were previously only available on larger high-end systems.
01:26 The modular design of the r series makes it possible to upgrade your instrument as your throughput requirements change.
01:35 For example an Octet® R2 system can be converted to an Octet® R4 in the field, doubling your throughput instantly and offering the perfect balance between performance and price.
01:46 You can even upgrade to an Octet® R8 to further increase throughput without the instrument ever leaving your lab.
01:53 This helps to protect your investment and future proof your workflows without the need to redesign assays and protocols as throughput increases.
02:04 the top of the range Octet® R8 offers high throughput analysis while still being within budget of most labs.
02:11 Intended for use in both pure research and GMP environments the Octet® R8 is designed to work seamlessly with 21 CFR part 11 compliance software simplifying and streamlining the compilation of documentation for regulatory filings.
02:26 This system also features advanced evaporation protection allowing long runtime experiments and high affinity binding kinetic studies to be performed without compromising on data quality.
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Octet®️ Platform: Instrument Showcase

Teach Me in 10

From cosmetics to medicine, there are various applications for peptides and proteins. These amino acid chains are often considered attractive therapeutic agents due to the possibility of creating tailored formulas for several applications.

To formulate a peptide or protein, you need to perform a chemical synthesis using mainly a solution-phase synthesis (SPS) or a solid-phase synthesis (SPPS). However, due to these compounds' complexity, there is no universal solution, and each compound requires a tailored approach based on its different characteristics.

Are you eager to discover which method is most suitable for formulating stable peptides? Join us for this episode of "Teach Me in 10" and delve into the following topics:
•	What are peptides and proteins? 
•	How can we synthesize peptides and proteins?
•	What happens to peptides after synthesis?
•	How to handle this material after purification?
•	The various applications of peptides and proteins?

Peptide Evolution: A How-To Guide from Synthesis to Final Formulation

In this informative Teach me in 10, Mariana Kiehl, a Senior Applications Scientist at Roche Sequencing & Life Science, introduces a novel single-tube workflow designed to seamlessly combine RNA and DNA sequencing from the same starting sample. She elucidates the intricacies of this method, highlighting its remarkable potential for providing a more profound insight into the intricate biological processes occurring within cells.

Utilizing FFPE tumor tissue sequencing as a compelling case study, Dr. Kiehl elucidates how this innovative approach conquers several common challenges that often impede the success of concurrent sequencing. These challenges encompass limited tissue availability for analysis, the inherent cellular diversity found in many tumors (even within adjacent sections), and the time-consuming need for separate DNA and RNA workflows, which can significantly extend the sample processing and analysis duration.

Experience the comprehensive breakdown of this cutting-edge technique by watching this Teach Me in 10.

Combined RNA & DNA Library Preparation for NGS-In a Single Tube

Luminex Corporation

Watch the recorded presentation from the Cytokines 2022 meeting by Angela Lombardi, PhD, Assistant Professor, Department of Medicine (Endocrinology) and Department of Microbiology & Immunology, Albert Einstein College of Medicine.

Learn more about the technology used in her research: https://www.luminexcorp.com/xmap-technology/#overview

Pancreas Derived Peptide Presentation by HLA-DQ in Diabetes—Angela Lombardi, PhD at Cytokines 2022

Get to know Luminex’s xMAP® Technology all over again. For 27 years, xMAP Technology has become known as the worldwide leader in bead-based multiplexing. With the new xMAP INTELLIFLEX®, we have re-energized the xMAP platform with a modern, fast, flexible and intuitive flow-based analysis system that can deliver twice the data in a single well as compared to previous instruments.

Join us to learn about the exciting applications of the xMAP INTELLIFLEX and its new dual reporter capability. We’ll describe some recently published studies that used this system, focusing on serological analyses. xMAP Technology enabled the multiplexed analysis of antigen-specific antibodies and the xMAP INTELLIFLEX’s simultaneous measuring of two antibody isotypes within a single well.

For Research Use Only. Not for use in diagnostic procedures.

Learning Objectives:

1. Refresh your knowledge of Luminex and xMAP bead-based multiplexing technology and how it has been used in life sciences research for the past 27 years.

2. Learn about the enhanced features and the novel second reporter channel of the innovative xMAP INTELLIFLEX, Luminex's newest instrument for xMAP Technology.

3. Learn about recent work that demonstrates the dual reporter functionality of the xMAP INTELLIFLEX for multiplexed serological analysis with antibody isotyping.

Learn more: https://www.luminexcorp.com/intelliflex/#overview

Accelerate Your Research With Luminex's xMAP INTELLIFLEX – Sherry Dunbar, PhD

Introducing the Luminex MAGPIX® System. This compact, fluorescent-based detection system is the simplest, most affordable, and most compact of Luminex’s xMAP® instruments. It can perform up to 50 different analytes in a single microtiter well and reads a 96-well-plate in just 60 minutes. Instead of using flow cytometry, the MAGPIX is a fluorescent imager that can perform quantitative analysis of both protein and nucleic acids. Based on Luminex's proven xMAP® Technology, the MAGPIX System uses color-coded magnetic microspheres as the substrate on which assays are performed. This video will walk you through how the system works.

Learn more: https://www.luminexcorp.com/magpix-system/#overview

Luminex MAGPIX® System

Luminex® xMAP® Technology makes up the basis of Luminex’s core business. This flexible technology is centered on the use of Luminex xMAP Microspheres.

xMAP was originally an acronym for multi-analyte profiling, where you solve for x. The surface of the microsphere offers multiple chemical attachment sites for biological capture molecules. When these molecules attach to the microsphere, it is called coupling, and this bonding can happen with both protein and nucleic acids, which make it a versatile and easy to use chemistry.

Learn more: https://www.luminexcorp.com/xmap-technology/#overview

Luminex xMAP® Technology Overview

Luminex® MAGPIX® Calibration & Verification How-To

Running a weekly calibration and daily verification on the MAGPIX® instrument in essential for maintaining instrument performance. 
Calibration normalizes the setting for the system and ensures optimal and consistent microsphere classification.
Performance Verification uses system controls to ensure that the analyzer is functioning properly with current calibration settings.  
Here is how to run the Calibration and Verification on the MAGPIX instrument.

Learn more: https://www.luminexcorp.com/magpix-system/#overview

Luminex® MAGPIX® Calibration & Verification How-To

Microspheres are an important part of the Luminex® library of assay and reagent offerings. Their special properties allow for multiplexing in a flexible way that maximizes the information that can be gathered in a single sample.
Learn more: https://www.luminexcorp.com/reagents-and-accessories/

Proteomics and Metabolomics

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