Proteomics and Metabolomics

Shimadzu Analytical & Measuring

With unparalleled speed, sensitivity and spatial resolution, Shimadzu’s iMScope QT imaging mass microscope clears the
way to next-generation mass spectrometry imaging. It enables fast acquisition of high-mass, high-resolution images at the optical and molecular level, can simultaneously visualize and analyze samples, and offers full fusion with morphology studies. Integration with the Q-TOF LCMS-9030 enables obtaining distribution information and quantitative data for comprehensive analysis and it's easy to switch between MS imaging configuration and LCMS.

High-End Imaging with an Unequalled Combination of Speed, Sensitivity, and Resolution 

Creative Proteomics videos

Glycan microarray has become a sensitive and versatile approach for the analysis of glycosylation changes in complex biological samples. It can not only investigate glycan-GBPs binding but also provide kinetic analysis of glycan–protein interactions. Nowadays, this application has rapidly expanded and now achieves successful discrimination of selective interactions between glycans and, not only proteins, but also viruses, bacteria and eukaryotic cells, and most recently even live cell responses to immobilized glycans. Glycan microarray can be used to discover functional glycans, characterize glycan-processing enzymes, and detect pathogens for diagnosis. It is also suitable for monitoring multiple molecular interactions with biomolecules where glycans are involved, offering a large variety of bioassay options.

As one of the leading companies in the omics field with over years of experience in omics study, Creative Proteomics provides Glycan Microarray Assay customized to your needs. https://www.creative-proteomics.com/services/glycan-microarray-assay.htm

Glycan Microarray Assay

Proteomics is a large-scale comprehensive study of a specific proteome, including information on protein abundances, the variations and modifications, as well as their interacting partners and networks. In discovery proteomics, proteome analysis can be performed in two different strategies, bottom-up and top-down approaches, respectively. In the bottom-up approach, a crude protein mixture undergoes protease digestion first, and then separation by liquid chromatography, followed by MS analysis. In the top-down method, proteins are characterized by MS without prior proteolysis. This type of approach has the advantage of providing greater sequence coverage, resolution of sequence ambiguities and preservation of PTMs. https://www.creative-proteomics.com/services/proteomics-service.htm

Protein Sequencing

Proteins are the critical players in molecular recognition at the core of all biological processes. They can interact with the other components of cells, such as small-molecule metabolites, nucleic acids, membranes, and other proteins to build supramolecular assemblies and elaborate molecular machines that perform all sorts of functions, from chemical catalysis and mechanical work to signaling and regulation. As one of the most important interactions, Protein-Protein Interactions have been studied widely. So far large scale protein-protein interactions have been identified, and all the generated data collected together in specialized databases, enables the creation of large protein interaction networks. https://www.creative-proteomics.com/services/protein-protein-interactions-analysis-service.htm

Techniques for Protein-Protein Interaction

Equipped with advanced tandem mass spectrometry for determination of lipid composition, powerful bioinformatic analytic systems, and a panel of experienced scientists and technicians, Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.
https://www.creative-proteomics.com/services/targeted-lipidomics.htm

Mass Spectrometry Based Lipidomics

Chromatography & Mass Spectrometry Solutions

Learn more about Thermo Scientific Vanquish Flex UHPLC systems and what they can do for you. The biocompatible Vanquish Flex UHPLC system brings exceptional analytical precision, detector sensitivity, and operational simplicity to enable unprecedented robustness and flexibility.

Thermo Scientific Vanquish Flex UHPLC System: Complete Flexibility

Aging (Aging-US)

Aging-US published this research paper in Volume 15, Issue 2, entitled, "Body weight influences musculoskeletal adaptation to long-term voluntary wheel running during aging in female mice" by the Department of Anatomy, Cell Biology and Physiology, School of Medicine, Indiana University, Indianapolis, IN; Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri, Kansas City, MO; Bone-Muscle Research Center, College of Nursing and Health Innovation, University of Texas, Arlington, TX; Department of Biostatistics and Health Data Science, School of Medicine, Indiana University, Indianapolis, IN; Department of Biomedical Sciences, School of Medicine, University of Missouri, Kansas City, MO; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN.

DOI - https://doi.org/10.18632/aging.204390

Corresponding authors - Lynda Bonewald - lbonewal@iu.edu, Michael J. Wacker - wackerm@umkc.edu 

Abstract

Frailty is the hallmark of aging that can be delayed with exercise. The present studies were initiated based on the hypothesis that long-term voluntary wheel running (VWR) in female mice from 12 to 18 or 22 months of age would have beneficial effects on the musculoskeletal system. Mice were separated into high (HBW) and low (LBW) body weight based on final body weights upon termination of experiments. Bone marrow fat was significantly higher in HBW than LBW under sedentary conditions, but not with VWR. HBW was more protective for soleus size and function than LBW under sedentary conditions, however VWR increased soleus size and function regardless of body weight. VWR plus HBW was more protective against muscle loss with aging. Similar effects of VWR plus HBW were observed with the extensor digitorum longus, EDL, however, LBW with VWR was beneficial in improving EDL fatigue resistance in 18 mo mice and was more beneficial with regards to muscle production of bone protective factors. VWR plus HBW maintained bone in aged animals. In summary, HBW had a more beneficial effect on muscle and bone with aging especially in combination with exercise. These effects were independent of bone marrow fat, suggesting that intrinsic musculoskeletal adaptions were responsible for these beneficial effects.

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Keywords - body weight, musculoskeletal adaptation, long-term, voluntary wheel running, aging

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

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Facebook - https://www.facebook.com/AgingUS/
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Body Weight Influences Musculoskeletal Adaptation to Running in Aging Mice

Edman degradation, which was developed by Pehr Edman, is a method to sequence amino acids in a peptide. It is the Cyclic degradation of peptides based on the reaction of phenylisothiocyanate with the free amino group of the N-terminal residue.  More at https://www.creative-proteomics.com/

Protein Sequencing – Edman Degradation

Proteomics studies play an increasing role in the field of biology. The use of mass spectrometry (MS) in combination with a range of separation methods is the main principal methodology for proteomics. The two principal approaches to identifying and characterizing proteins using MS are the “bottom-up”, which analyze peptides by proteolytic digestion, and “top-down”, which analyze intact proteins.  More at https://www.creative-proteomics.com/

Proteomics and Metabolomics

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