Next-Generation Sequencing

Teach Me in 10

Next-generation sequencing (NGS) target enrichment methods provide solutions to focus on and sequence a small portion of the human genome. This saves sequencing and analysis costs for labs that study genetic diseases. These savings typically range from approximately 100 to 100,000 fold, depending on the application and the amount of the genome being targeted. Until now, there have been two primary target enrichment methods used in the field.
 Hybridization capture methods are considered high-performance solutions that tend to require cumbersome steps and an overnight hybridization incubation for optimal performance. On the other hand, amplicon technologies are considered fast and easy but generally do not have the same level of performance compared to capture technologies. Primer Extension Target Enrichment (PETE) is a newly developed technology that uses DNA polymerase extension reactions to provide a high-performance target enrichment solution that is easy to use and can be performed in a single day. 

In this Teach Me in 10, Brian Godwin, director of reagent and assay development at Roche sequencing, joins Lucy Lawrence to give us an overview of how each of the three target enrichment technologies work along. Godwin also shares a description of common target enrichment performance metrics.

Introducing Primer Extension Target Enrichment for NGS With Brian Godwin

Next Generation Sequencing (NGS) target enrichment methods provide solutions to focus on and sequence a small portion of the human genome.  This saves sequencing and analysis costs for labs that study genetic diseases.  These savings typically range from approximately 100 to 100,000 fold depending on the application and the amount of the genome being targeted. 

Until now, there have been two primary target enrichment methods used in the field.  Hybridization capture methods are considered high-performance solutions that tend to require cumbersome steps and an overnight hybridization incubation for optimal performance.  On the other hand, amplicon technologies are considered fast and easy but generally do not have the same level of performance compared to capture technologies.

In this episode of Teach Me in 10, Lucy Lawrence is joined by Brian Godwin the Director of Reagent and Assay Development at Roche Sequencing and he shares methods, performance metrics and application data surrounding NGS Target Enrichment. 

Introducing Primer Extension Target Enrichment for NGS with Brian Godwin 

Oxford Nanopore Technologies

Oxford Nanopore sequencing devices enable direct, real-time analysis of short to ultra-long fragments of DNA/RNA in fully scalable formats. 

It’s possible to target regions of DNA in real time using adaptive sampling on nanopore devices. The nanopore has the ability to read the strand sequence in real time, if it is not the strand of interest, the strand is ejected, but if it is, the nanopore recognises this and continues to read the strand. Researchers then get more of the sequence they are interested in within a shorter period of time, allowing faster and easier analysis. 

Adaptive Sampling: Target Regions of DNA in Real Time

CD Genomics

CD Genomics has been providing the accurate and affordable whole genome sequencing service for couple of years. We combine both Illumina (short reads) and PacBio (long reads) platforms to achieve whole genome de novo assemblies and re-sequencing for viruses, microbes, plants, animals and humans.	https://www.cd-genomics.com/Whole-Genome-Sequencing.html

Methods and Workflow of Whole Genome Sequencing

RNA-Seq is the premier tool for mapping and quantifying transcriptomes by utilizing next-generation sequencing (NGS) technology. The transcriptome refers to the complete set of transcripts in a cell, which provides information on the transcript level for a specific developmental stage or physiological condition. Understanding the transcriptome is necessary for interpreting the functional elements of the genome and understanding development and disease. The key purpose of transcriptomics includes cataloging all species of transcripts; determining the transcriptional structure of genes; and quantifying the expression levels of each transcript under different conditions.

The Development and Workflow of RNA-seq, Learn more at https://www.cd-genomics.com/resourse-Bioinformatics-Workflow-of-RNA-Seq.html

Principle and Workflow of RNA Sequencing

Highly integrated single-base resolution DNA methylation patterning; Providing insights into gene cell-fate commitment and reprogramming, as well as gene regulation; Identifying novel epigenetic markers and targets for disease· Unable to distinguish between 5mC and 5hmC.
More information at https://www.cd-genomics.com/5mc-5hmc-sequencing-1.html

5mC and 5hmC Sequencing Methods

CD Genomics is committed to providing novel NGS services enabling researchers to explore structure and function of the microbial community in a high-resolution and culture-independent manner using technology from Illumina and PacBio. In addition, we also offer the service to sequence the genome of the individual cultured bacteria, fungi, phage or virus, whether do novo or re-sequencing. https://www.cd-genomics.com/Microbial-Genomics.html

Microbial Genomics Solutions at CD Genomics

CD genomics can help you analyze genetic variation efficiently to meet diverse research needs. By utilizing Affymetrix and Illumina array platforms, we support high-throughput and multiplex processing to meet diverse research needs, delivering high-quality data at a low per-sample cost. https://www.cd-genomics.com/SNP-Microarray.html

SNP Genotyping Technologies

To support the increasing research interest in small RNA, CD Genomics is offering the qualified small RNA sequencing service that covers novel small RNA discovery, mutation characterization, and expression profiling of small RNAs by leveraging of advanced NGS technologies and data analysis pipeline.	https://www.cd-genomics.com/Small-RNA.html

Next-Generation Sequencing

Refine By

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.