Chromatography

SiliCycle Inc.

This Q&A video will show you the two different ways to determine how much scavenger will be needed in your purification based on your solution parameters.

What are metal scavengers?
Metal Scavengers are functionalized silica gels designed to react and bind metal complexes. They are used to remove metallic impurities from your chemistry.

Metal scavengers are offered in various formats:

- as bulk (SiliaMetS functionnalized silica gels)
https://www.silicycle.com/products/metal-scavengers

- packed in "flash" cartridges (SiliaSep)
https://www.silicycle.com/products/siliasep-flash-cartridges

- packed in SPE cartridges (SiliaPrep)
https://www.silicycle.com/products/silicycle-sample-preparation-products/spe-cartridges

- packed in flow-through fixed bed cartridges (E-PAK)
https://www.silicycle.com/products/e-pak-purification-cartridges

FAQ How to calculate the right amount of scavenger to remove metal / organic impurities

Analytical Cannabis 

Speaking at The Science of Cannabis Cultivation Online Symposium in December 2021, Stephen Goldman, Chief Science Officer at Kaycha Labs explored pesticide testing in cannabis and hemp.

Abstract: Pesticide and mycotoxin testing regulations differ in each state. The applicable pesticides used can differ by crop (hemp vs. cannabis) and pesticide effectiveness on cannabis and hemp in the first place is currently in doubt. Each pesticide's risk profile is different based on product type, route of delivery, physicochemical properties. Inside the laboratory, different extraction methods can lead to different recoveries and different instrumentation has different detection limits. This talk will cover how cultivators and laboratories can reliably mitigate the risk of these toxic compounds in their products and ensure regulatory compliance and robust methodology.

Pesticides in Cannabis and Hemp Testing. Finding the Right Tool for the Job

Agilent - LC

Designed as a process analytical technology (PAT) tool for automated process monitoring, the Agilent 1260 Infinity II Prime Online LC System provides real-time data for greater control and faster understanding of your processes. Watch this video to learn more.

Keep Pace with Your Processes

Introducing Agilent Feed Injection. Watch this video to learn how this novel LC injection technique can help you minimize the negative effects of strong sample solvent, support rapid gradients, and provide great injection precision for sub-1 µL injections.

Full Control Over Challenging Process Samples

Creative Bioarray

Parallel Artificial Membrane Permeability Assay (PAMPA) is a cell-free permeation model used to predict passive drug transport. Passive diffusion is an important factor in determining the absorption of orally administered compounds in the gastrointestinal tract (GIT), penetration of the blood-brain barrier (BBB), and general transport across cell membranes.

https://dda.creative-bioarray.com/parallel-artificial-membrane-permeability-assay-pampa-protocol.html

PAMPA Permeability Assay

Technology Networks

Speaking at the ‘Advances in Proteomics & Metabolomics’ online symposium 2021, Professor Matthias Mann from Director Dept. Proteomics and Signal Transduction Max Planck Institute of Biochemistry; presented his talk on ‘Ultra-High Sensitivity Proteomics for Digital Cancer Pathology.’
Abstract: Great instrumental advances continue to be made in mass spectrometry-based proteomics and I will discuss a few of these, including advances in algorithms and bioinformatics and focusing on the new tims-TOF technology, which now allows ultra-sensitive analysis of single cells.

Ultra-High Sensitivity Proteomics for Digital Cancer Pathology

dandy Innovations

Dear scientists, get ready for some good news about filtering materials in the lab! For the first time ever, you can work in the lab without experiencing any hand pain whatsoever, thanks to dandyVice! It’s the only solution for filtering materials in a syringe without straining the thumb, thereby preventing long-term pain and damage. dandyVice, a registered patent, is now available for sale, by direct order, on the dandy Innovations website.
https://dandy-innovations.com/product/dandyvice/

Still using you hand to filter? Use the dandyVice! no more thumb pain!

Created with proprietary technology, E-PAK radial flow cartridges provide rapid adsorption kinetics and processing capacities for laboratory, pilot and commercial operations. They are designed for use with both organic and aqueous solvents, and incorporate design features useful for the production of active pharmaceutical ingredients (API).

Features include:
• Proven cartridge design to ensure rapid, simple & reliable technology
• Fixed-bed design ensuring safer handling, clean-up and disposal
• Large adsorbent capacity in small area footprint increases product recovery & reduces solvent requirements

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges


Music Credit: 
Brighter Days by Markvard & Kvarmez

E-PAK cartridge 3D animation  of API filtration

Watch a time lapse of  a dye mixture having each colour separated and isolated via flash chromatography using bare silica spherical gel.  

Procedure 
Cartridge : FLH-10095D-A-ISO40 (https://www.silicycle.com/products/siliasep-flash-cartridges/portfolio/siliasep-premium/flh-10095d-a)
Solvent : A = Methanol, B = Ethyl Acetate 
Conditioning : 100% Ethyl Acetate, 1 min at 100 mL/min 
Injection : 0.5 mL via liquid loading 
Gradient : 
-Segment 1 : 100%B, for 1 min at 100 mL/min 
-Segment 2 : 100-55% B, 5 min at 100 mL/min 
-Segment 3 : 55% B, 6 min at 100 mL/min 

 Our flash chromatography products offer high levels of performance and reproducibility. The flash media consistently has a tight particle size distribution with low fines and extremely low metal ion content, for more predictable and easier separations.

Using SiliCycle SiliaSep cartridges, you can purify your sample base on different mechanisms depending on the properties or your compound. Learn more about SiliaSep PREMIUM
https://www.silicycle.com/products/siliasep-flash-cartridges/portfolio/siliasep-premium

Chromatography

Refine By

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.