Chromatography

Teach Me in 10

As per- and polyfluoroalkyl compounds become more prominent, the US Environmental Protection Agency (EPA) are developing more stringent regulations for PFAS monitoring. 

In this Teach Me in 10, we hear from field application scientist Marc Elie on the challenges of PFAS analysis in matrices including drinking water and wastewater and how to mitigate interference and contamination on LC-MS/MS instrumentation. 

After watching, you’ll understand the differences between analysis for US EPA Methods 537.1, 533 and 1633 as well as the additional difficulties of PFAS analysis in non-potable water.

Mitigate Interference and Contamination in PFAS Analysis

CloudLIMS

LIMS webinar focuses on how a LIMS can help testing laboratories manage data, automate laboratory workflows, and meet regulatory guidelines and quality standards seamlessly. Details on: https://cloudlims.com/resources/lims-webinar/

LIMS Webinar: Manage Testing and Laboratory Workflows with LIMS

Due to the unique ion exchange mechanisms and separation chemistry of analytes, it’s important to understand how sample preparation methods can contribute to the success of ion chromatography experiments. Sample preparation is crucial to obtaining the most accurate results - regardless of the sample type. 

This Teach Me in 10 we speak with Dr Yan Liu, Director, of Chemistry R&D for Ion Chromatography at Thermo Fisher Scientific. We cover some of the common sample preparation techniques used in ion chromatography and explore methods to accelerate target analyte determination.

Simple and Effective Sample Preparation Techniques for Ion Chromatography

Chromatography & Mass Spectrometry Solutions

An LC-HRAM-MS method with Thermo Scientific™ TurboFlow™ online SPE was developed and validated for the quantitation of immunosuppressants. Online SPE efficiently extracts analytes while removing other components in a crude supernatant, significantly simplifying sample preparation. The use of full-scan data-dependent MS2 acquisition allows for high-resolution selected-ion monitoring quantitation of the analytes, confirming the analytes using MS2 fragmentation, as well as monitoring the signal intensities of major immunosuppressant metabolites.

A Therapeutic Drug Monitoring Assay of Immunosuppressants using TurboFlow LC and High-Resolution Mass Spectrometry

Detect an ever-increasing number of substances at ever-decreasing concentrations. The Thermo Scientific™ Tox Explorer™ Collection helps the world’s toxicology community in forensics, clinical research, employee drug testing, and sports anti-doping navigate with ease to solve complex analytical challenges and increase productivity. Tox Explorer Collection is an all-in-one toxicology solution. Incorporating the TSQ Quantis Plus mass spectrometer for drugs of abuse testing enables sensitive and reproducible analytical measurement needed for confirmation for compounds covering a range of compound classes and polarities.

Targeted Screening and Quantitation With a Tox Explorer Triple Quadrupole LC-MS Workflow

Oncotarget

Oncotarget published this trending research paper in Volume 13, entitled, "Nectin-4 is widely expressed in head and neck squamous cell carcinoma" by researchers from the Institute of Pathology, University Hospital Bonn (UKB), Bonn, Germany; Department of Otorhinolaryngology, University Medical Center Bonn (UKB), Bonn, Germany; Department of Haemato-oncology, University Hospital Bonn (UKB), Bonn, Germany

DOI - https://doi.org/10.18632/oncotarget.28299

Correspondence to - Glen Kristiansen - glen.kristiansen@ukbonn.de 

Abstract

Purpose: Nectin-4 has been successfully established as a target molecule in locally advanced and metastatic bladder cancer. An antibody-drug conjugate (enfortumab-vedotin) directed against nectin-4 has shown marked tumor remission rates in this tumor type, which is known for high expression rates of nectin-4. As head and neck cancer and urothelial carcinomas share morphological and molecular similarities, we aimed to evaluate Nectin-4 expression in head and neck squamous cell carcinoma (HNSCC).

Material and Methods: A previously described and clinically characterized cohort of HNSCC (n = 159) was analyzed by immunohistochemistry for Nectin-4 expression. The expression data was correlated to clinico-pathological parameters including patient outcome.

Results: Nectin-4 was found in 86.2% of HNSCC, with medium/high expression seen in 32.7% of cases. Non smokers and p16 positive HNSCC showed a higher expression of Nectin-4 (p < 0.005). There was no correlation of Nectin-4 with grading or tumor stage. Nectin-4 positive tumors showed a significant better survival (log rank p = 0.006).

Conclusions: Similar to urothelial carcinoma, Nectin-4 is found in the majority of HNSCC, which clearly warrants further studies to clarify if HNSCC also respond to targeted therapy with enfortumab-vedotin. Moreover, expression of Nectin-4 is associated with HPV infection and may serve as a prognostic marker in HNSCC.

Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.28299

Keywords - Nectin-4, enfortumab-vedotin, HNSCC, p16

About Oncotarget

Oncotarget is a primarily oncology-focused, peer-reviewed, open access journal. Papers are published continuously within yearly volumes in their final and complete form, and then quickly released to Pubmed. On September 15, 2022, Oncotarget was accepted again for indexing by MEDLINE. Oncotarget is now indexed by Medline/PubMed and PMC/PubMed.

To learn more about Oncotarget, please visit https://www.oncotarget.com and connect with us:

SoundCloud - https://soundcloud.com/oncotarget
Facebook - https://www.facebook.com/Oncotarget/
Twitter - https://twitter.com/oncotarget
Instagram - https://www.instagram.com/oncotargetjrnl/
YouTube - https://www.youtube.com/OncotargetYouTube
LinkedIn - https://www.linkedin.com/company/oncotarget
Pinterest - https://www.pinterest.com/oncotarget/
Reddit - https://www.reddit.com/user/Oncotarget/

Media Contact
MEDIA@IMPACTJOURNALS.COM
18009220957

Trending With Impact: Nectin-4 is Widely Expressed in Head and Neck Squamous Cell Carcinoma

HTA srl

UNIVERSAL HTA AUTOSAMPLER
HTA autosamplers can be interfaced with almost any gas chromatograph and they can serve both the front and rear injector in most supported GCs.

Furthermore HTA autosamplers are compatible with all the Chromatography Data Systems:

HTA autosamplers can be perfectly integrated with any system, causing almost no change to lab current habits.
For every data system, HTA offers the Basic Integration. For some of the most popular data systems, HTA also offers Advanced Integration by purchasing an additional extension or driver.

Integration with Chromatography Data System

Ambient ionization mass spectrometry techniques that enable direct, gentle, and rapid analysis of samples offer exciting opportunities to provide clinicians with rich molecular data to enhance decision-making. Here, an overview of several ongoing clinical projects in the Eberlin laboratory related to the development of a handheld MS-based device, the MasSpec Pen technology, for improving patient care will be discussed. In particular, I will describe results centered around our efforts in employing the MasSpec Pen for intraoperative tissue analysis and cancer detection, identification of infectious microorganisms, and detection of drugs of abuse. Current challenges and opportunities towards incorporating this technology into clinical practice will also be addressed.

From the OR to the Microbiology Lab: Advanced Development of the MasSpec Pen for Broad Clinical Use

Therapeutic drug monitoring (TDM) represents significant workflow demands and revenue generation for clinical laboratories. A high throughput, validated paper spray mass spectrometry assay using the Thermo Scientific™ VeriSpray™ source is presented as a facile and rapid TDM strategy for the direct, quantitative analysis of clozapine and its metabolite in human serum samples. Quantitative results obtained compare favourably with a validated clinical LC-MS assay.
For Research Use Only. Not for use in diagnostic procedures. Thermo Scientific™ Orbitrap™ mass spectrometers are intended for General Laboratory Use Only, and not for Diagnostic or Clinical Use.

Chromatography

Refine By

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.