Chromatography

HTA srl

HT4000A sample prep workstation can automate chemical and biological reaction, through the handling of derivatization operations followed by the incubation in a temperature controlled reaction cell. Final reaction product can be directly injected into HPLC for on-line analysis.

HT4000A - chemical/biological reaction automation before HPLC injection

Analytical Cannabis 

Speaking at The Science of Cannabis 2020 Online Symposium, Rob Thomas, Principal Consultant at Scientific Solutions presented his talk on heavy metals regulation and how lessons from the pharmaceutical sector could be transferred to the cannabis industry.<br/><br/>Abstract: The lack of federal oversight with regard to medicinal cannabis and hemp products in the US has meant that it has been left to the individual states to regulate its use. Medical marijuana is legal in 33 states, while 11 states allow its use for adult recreational consumption. The sale of these products is strictly regulated by their THC and CBD content, depending on their use. However, it’s also critical to monitor levels of contaminants such heavy metals, as the cannabis plant is known to be a hyper-accumulator of heavy metals in the soil. Unfortunately, there are many inconsistencies with heavy metal limits in different states where medical cannabis is legal. Some states define four heavy metals while others specify up to eight. Some are based on limits directly in the cannabis, while others are based on consumption per day. Others take into consideration the body weight of the consumer, while some states do not even have heavy metal limits. So clearly there is a need for consistency across state lines, in order that consumers know they are using products which are safe to use. This presentation will take a closer look at how the pharmaceutical industry changed its 100-year old sulfide precipitation test for a small group of heavy metals, to finally arrive at a list of 24 elemental impurities using plasma spectrochemical techniques. The cannabis industry can learn a great deal from this process to not only understand the many potential sources of heavy metal contamination, but also how the final cannabis products can be contaminated by the manufacturing equipment, the extraction process and the delivery systems used.

Regulating Heavy Metals in Cannabis: What We Can Learn From the Pharmaceutical Industry

Speaking at the Analytical Cannabis Expo East Online 2020, Dr Ben Armstrong, Laboratory Director at Juniper Analytics, presented his talk on how to mitigate matrix effects in cannabis and hemp analysis.

Abstract: The food and pharmaceutical industries established methods for pesticide testing many years ago. However, cannabis is unique in that it contains over 500 compounds not found in other fruits and vegetables. A variety of extraction methods are currently used for sample preparation, each with its own benefits and disadvantages. This is further complicated by the large variety of sample matrices that are presented for pesticide residue analysis. Accuracy, precision and sensitivity of the method are frequently compromised by the presence of matrix effects. Techniques can be used to mitigate the matrix effects that each product presents, however this comes with its own set of challenges. This talk will discuss extraction methods used in the cannabis and hemp industry and highlight strategies for mitigating matrix effects, the need for improved extraction protocols, and validated methods.

Strategies to Mitigate Matrix Effects in Cannabis and Hemp Pesticide Residue Analysis

Shimadzu Analytical & Measuring

Management of the mobile phase is of great importance in HPLC analysis. Making sure you have sufficient mobile phase in the system—before batch analysis—is critical to keeping your lab running smoothly. For this reason, Shimadzu’s Nexera UHPLC series enables real-time monitoring of mobile phase levels to ensure maximum uptime. The instrument monitors solvents gravimetrically and notifies lab personnel if there isn’t enough mobile phase to complete the batch run. Watch the video to learn about this key operation that provides maximum uptime and productivity.  

Real-Time Mobile Phase Monitoring with the Nexera UHPLC Series

With the passing of the 2018 Farm Bill legalizing hemp and different US states legalizing Cannabis products, farmers are allowed to grow cannabis and hemp. Like any other agricultural crop, pesticides are applied to cannabis and hemp plants to protect it from pests and improve growth yield. Chronic exposure to pesticides can lead to serious health risks and therefore pesticide analysis in Cannabis and hemp is important for consumer safety and quality control. Among all of states in USA, California has issued most stringent action limits for 66 pesticides residues in cannabis flower, cannabis infused edibles, concentrates and hemp.  Normally pesticide analysis in cannabis and other food matrices is done by both GCMSMS and LCMSMS since some nonpolar and chlorinated pesticides such as pentachloronitrobenzene, chlordane and others are difficult to ionize with electrospray source used in LCMSMS systems.   We would demonstrate how we measured these compounds at low levels using LCMSMS with APCI source. The ionization mechanism of chlorinated pesticides such as pentachloronitrobenzene and others using LCMSMS system with APCI source would be presented. We analyzed all of 66 pesticides (including very hydrophobic and chlorinated pesticides typically analyzed by GCMSMS) spiked in  hemp and cannabis samples  well below the action limits specified by California State. Hemp and cannabis matrix is challenging and therefore it causes ion suppression and matrix inference for response of some of pesticides. We would demonstrate approaches to mitigate these matrix effects from cannabis and hemp. Sample preparation is the main bottleneck for achieving the high throughput analysis of pesticides in cannabis/hemp testing labs. For sample preparation, we used solvent extraction since it is a quick, cheap and easy way to achieve high extraction recovery in comparison to other time-consuming sample preparation techniques like solid phase extraction (SPE) and QuEChERS. Also, the performance of LC-MS/MS method was checked by conducting blind emerald scientific’s proficiency test for 66 pesticides in hemp. The proficiency test report generated with LCMSMS method showed acceptable results for all of 66 pesticides in hemp with no false positives and negatives.

Overcoming Challenges associated with analysis of all of pesticides regulated by California

CEM_Corporation

Our host, Benedict Liu, is a little embarrassed in this episode of Will it Extract? From high school flashbacks to World War II plastic surgery tricks, liquid concealer has played many varying roles. Will Ben overcome his shyness and work with EDGE to correctly prep the sample for analysis of parabens, PFAS, and other potentially harmful chemicals?

Getting the sample into the Q Cup (and not on the counter) is Ben's first challenge. As a rather viscous liquid, he wants to make sure it will stay in the Q Cup long enough to be extracted. What sample prep will be involved? And most importantly, can the EDGE handle it? Tune in to see if EDGE can cover!

Will it Extract? Watch and find out!

Our Applications Teams, Sales Support Staff, and Service Personnel are all available and able to help. Need to contact them? Don't hesitate:
Molecular Method Support: molecular.support@cem.com

Sales Support: info@cem.com

Service/Service Contracts/Hardware: service@cem.com

Will it Extract? Liquid Concealor

Our host, Benedict Liu, is getting ready for Halloween, including picking out his costume, a ghoul, and that means eye shadow. You can't be a good ghoul without good eye shadow. He works with EDGE to correctly prep the sample for analysis of parabens, PFAS, and other potentially harmful chemicals.

Eye shadow does present some interesting challenges. It is a powder that is quite light. Making certain it stays in the Q-Cup will be one of Ben's highest priorities. What sample prep is involved? And most importantly, can the EDGE handle it? Tune in to see if EDGE delivers a trick or a treat!

Will it Extract? Watch and find out!

Our Applications Teams, Sales Support Staff, and Service Personnel are all available and able to help. Need to contact them? Don't hesitate:
Molecular Method Support: molecular.support@cem.com

Sales Support: info@cem.com

Service/Service Contracts/Hardware: service@cem.com

Will it Extract? 

Benjamin Liu and EDGE take us on a virtual trip halfway around the world to Japan. Bonito flakes are a staple in Japanese cooking. They require quite a long process to properly prepare them for consumption. Once ready to go, they are tested for both mycotoxins and fat. The question is: are they as difficult to test as they are to manufacture? What sample prep is involved? And most importantly, can the EDGE handle it?

Our Applications Teams, Sales Support Staff, and Service Personnel are all available and able to help. Need to contact them? Don't hesitate:
Molecular Method Support: molecular.support@cem.com

Sales Support: info@cem.com

Service/Service Contracts/Hardware: service@cem.com

Will it Extract? Bonita Flakes

Did you know that Thursday is National Root Beer Float Day? Have you been missing out on the past few years? Don't worry, Ben has you covered. Tune in to learn a bit more about root beer and plastics, one of the common means of holding root beer for mass distribution. Ben puts PET to the test to see if the EDGE can handle it. PET, PVC, polyethylenes, and more all follow very similar sample prep processes to analyze for UV stabilizers, slip additives, phthalates, and more. So, the question, as always is...Will it Extract? Watch and find out!  

For more product information visit https:https://cem.com/edge/

Want more CEM updates and videos? Subscribe to our channel: https://www.youtube.com/c/CEMCorporation

Check out CEM's Resource Library to download various Application Notes: https://cem.com/en/literature?application=Extraction&literature_type=Application_Notes

Explore CEM's Solvent Extraction page: https://cem.com/en/solvent-extraction

Connect with us
Follow @CEMcorporation on Twitter: https://twitter.com/CEMcorporation
Follow and Like on Facebook: https://www.facebook.com/CEMCorporation
Follow CEM on LinkedIn: https://www.linkedin.com/company/cem-...
Subscribe to CEM on YouTube: https://www.youtube.com/c/CEMCorporation
Follow CEM on Instagram: https://www.instagram.com/cemcorporation/

Chromatography

Refine By

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.