Imaging/Microscopy

Thermo Fisher Scientific, Biosciences

In this video we'll discuss how to select the optimal PCR plate for high-throughput PCR and qPCR applications. Many Thermo Fisher PCR plates can be used for both PCR and qPCR reactions. There are several PCR plate formats that are compatible with the grippers, or paddles, found on the liquid handling automation systems. This video will help you choose the correct PCR plate for your high-throughput application.

You can find everything you need for these applications by visiting ThermoFisher.com/pcrplastics

How to select the best PCR plate for high-throughput PCR and qPCR applications

In this video we'll introduce and provide instruction for the use of two Applied Biosystems adapter trays. These trays are designed for use with Applied Biosystems thermal cyclers when using tube strips with separate cap strips, or tubes and tube strips with attached caps. Use of these adapter trays is recommended in order to prevent tube deformity, or crushing, during thermal cycling while ensuring optimal PCR yield.

You can find everything you need for these applications in your Applied Biosystems thermal cycler starter kit, or by visiting ThermoFisher.com/pcrplastics.

How to use adapter tray retainers in the Applied Biosystems thermal cycler starter kit

In this video we'll discuss how to use each accessory and tool that comes with the purchase of a new Applied Biosystems thermal cycler.

These tools and accessories can be found in the PCR starter kit. You can find everything you need for setting up your PCR reaction in your Applied Biosystems thermal cycler starter kit or on ThermoFisher.com/pcrplastics.

How to use the tool in the thermal cycler starter kit

Aging

This week's cover paper of Aging (Volume 13, Issue 13) is entitled, "A model of the aged lung epithelium in idiopathic pulmonary fibrosis," by researchers from The Jane and Leonard Korman Respiratory Institute, Philadelphia, PA; Rutgers – New Jersey Medical School, Newark, NJ; Baylor College of Medicine, Houston, TX.

Abstract:
Idiopathic pulmonary fibrosis (IPF) is an age-related disorder that carries a universally poor prognosis and is thought to arise from repetitive micro injuries to the alveolar epithelium. To date, a major factor limiting our understanding of IPF is a deficiency of disease models, particularly in vitro models that can recapitulate the full complement of molecular attributes in the human condition. In this study, we aimed to develop a model that more closely resembles the aberrant IPF lung epithelium. By exposing mouse alveolar epithelial cells to repeated, low doses of bleomycin, instead of usual one-time exposures, we uncovered changes strikingly similar to those in the IPF lung epithelium. This included the acquisition of multiple phenotypic and functional characteristics of senescent cells and the adoption of previously described changes in mitochondrial homeostasis, including alterations in redox balance, energy production and activity of the mitochondrial unfolded protein response. We also uncovered dramatic changes in cellular metabolism and detected a profound loss of proteostasis, as characterized by the accumulation of cytoplasmic protein aggregates, dysregulated expression of chaperone proteins and decreased activity of the ubiquitin proteasome system. In summary, we describe an in vitro model that closely resembles the aberrant lung epithelium in IPF. We propose that this simple yet powerful tool could help uncover new biological mechanisms and assist in developing new pharmacological tools to treat the disease.

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DOI -  https://doi.org/10.18632/aging.203291

Full text - https://www.aging-us.com/article/203291/text

Correspondence to: Ross Summer email: Ross.Summer@Jefferson.edu and Freddy Romero email: Freddy.RomeroVasquez@bcm.edu

Keywords: aging, IPF, mitochondria, proteostasis, epithelial cells

About Aging

Launched in 2009, Aging publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at http://www.Aging-US.com​​ or connect with us on:

Twitter - https://twitter.com/AgingJrnl​
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Aging is published by Impact Journals, LLC please visit http://www.ImpactJournals.com​​ or connect with @ImpactJrnls

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A New Model System for Idiopathic Pulmonary Fibrosis

In this video we'll discuss how to properly seal a plate using two methods: the use of an adhesive film and cap strips.

You can find everything you need for these applications in your Applied Biosystems Thermal Cycler starter kit or at ThermoFisher.com/pcrplastics.

How to seal an Applied Biosystems PCR plate

In this video, we'll discuss how to properly seal a plate using two methods: use of an adhesive film and cap strips.

You can find everything you need by visiting ThermoFisher.com/thermoscientificplastics

How to seal a Thermo Scientific PCR plate

Oncotarget

View the latest oncology-focused research published in this week’s issue of Oncotarget, Volume 12, Issue 13.
https://www.oncotarget.com/archive/v12/i13/

Cover Paper - “Oncogenic transformation of NIH/3T3 cells by the overexpression of L-type amino acid transporter 1, a promising anti-cancer target” 
https://doi.org/10.18632/oncotarget.27981


News - “A new class of radiosensitizers for glioblastoma”
https://doi.org/10.18632/oncotarget.27970 (PDF Download)

Research Paper - “A platform for locoregional T-cell immunotherapy to control HNSCC recurrence following tumor resection” 
https://doi.org/10.18632/oncotarget.27982


Research Paper - “Human papilloma virus circulating tumor DNA assay predicts treatment response in recurrent/metastatic head and neck squamous cell carcinoma” https://doi.org/10.18632/oncotarget.27992

Research Paper - “Association of high-sensitivity C-reactive protein and odds of breast cancer by molecular subtype: analysis of the MEND study” https://doi.org/10.18632/oncotarget.27991

Research Paper - “Glucocorticoid receptor antagonism promotes apoptosis in solid tumor cells”
https://doi.org/10.18632/oncotarget.27989



Research Paper - “TERT and its binding protein: overexpression of GABPA/B in high grade gliomas” https://doi.org/10.18632/oncotarget.27985

Review - “Cross-talks in colon cancer between RAGE/AGEs axis and in-flammation/immunotherapy” https://doi.org/10.18632/oncotarget.27990

Review - “Cancer-epigenetic function of the histone methyltransferase KMT2D and therapeutic opportunities for the treatment of KMT2D-deficient tumors” https://doi.org/10.18632/oncotarget.27988

Research Perspective - “Targeting super-enhancers reprograms glioblastoma central carbon metabolism” https://doi.org/10.18632/oncotarget.27938

Editorial Perspective - “Polo-like kinase inhibition as a therapeutic target in acute myeloid leukemia” https://doi.org/10.18632/oncotarget.27919 (PDF Download)

Editorial - “The formation of pre-effectors in the steady state opens a new perspective for cancer immunosurveillance” https://doi.org/10.18632/oncotarget.27967 (PDF Download)

Editorial - “Mechanisms of gefitinib-induced interstitial pneumonitis: why and how the TKI perturbs innate immune systems?” https://doi.org/10.18632/oncotarget.27958 (PDF Download)

Keywords - oncogenicity,  glioblastoma, head and neck cancer, immunotherapy, tumors, glioma, colon cancer, epigenetics, cancer immunosurveillance, gefitinib, AML, breast cancer, cancer, science, research, oncology


About Oncotarget

Oncotarget is a bi-weekly, peer-reviewed, open access biomedical journal covering research on all aspects of oncology. 

To learn more about Oncotarget, please visit https://www.oncotarget.com/ or connect with:

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Oncotarget is published by Impact Journals, LLC. Please visit https://www.impactjournals.com/ or connect with @ImpactJrnls

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Table of Contents - Volume 12, Issue 13

Oncotarget published this research paper on August 11, 2020, entitled, “Survival after resection of brain metastases with white light microscopy versus fluorescence-guidance: A matched cohort analysis of the Metastasys study data,” by researchers from the University of Medicine Goettingen, Goettingen, Germany; University Medical Center Leipzig, Leipzig, Germany; University Hospital Muenster, Muenster, Germany.

The cover for issue 32 of Oncotarget features Figure 2, "This figure depicts overall survival and local in-brain recurrence-free survival in the study's subgroups," by Hussein, et al. which reported that the aim of the present study is to assess whether the use of 5-ALA has an impact on local recurrence or survival compared to conventional white light microscopic tumor resection.

Two groups were compared:
In the “white light” group, resection was performed with conventional microscopy.
In the 5-ALA group, fluorescence guided peritumoral resection was additionally performed after standard microscopic resection.
Local in-brain recurrence occurred in 21/175 patients with a rate of 15/119 in the white light and 6/56 in the 5-ALA group.

The use of 5-ALA did not result in lower in-brain recurrence or mortality compared to the use of white light microscopy.

Dr. Bawarjan Schatlo from the Department of Neurosurgery at The University of Medicine Goettingen said, "Metastatic brain disease is more common than primary brain tumors."

Another group made the case for extending tumor resection 5 millimeters into peritumoral tissue to perform a so-called supramarginal resection.

Its aim is to prolong progression-free survival through radical resection and improved local tumor control.

In a series of 52 patients, Kamp and colleagues detected positive fluorescence in 62% of resected cerebral metastases.

Thus, the utility and importance of using methods to improve local control of brain metastases remains an unresolved issue.

The aim of the current study was to compare survival and local recurrence in a cohort of patients who underwent surgery for brain metastases with 5-ALA fluorescence microscopy to one that was operated using microscopic white light only.

The Schatlo Research Team concluded in their Oncotarget research paper, "the present study confirmed that regardless of surgical adjunct, radiotherapy is strongly associated with improved survival."

To date, this research paper has generated an Altmetric Attention Score of 48. The Altmetric Attention Score provides an at-a-glance indication of the volume and type of online attention the research has received. 

Top Oncotarget publications rated by Altmetric Attention Score - https://www.oncotarget.com/news/altmetric/

Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.27688

DOI - https://doi.org/10.18632/oncotarget.27688

Full text - https://www.oncotarget.com/article/27688/text/

Correspondence to - Bawarjan Schatlo - bawarjan.schatlo@med.uni-goettingen.de

Keywords - brain tumor, brain metastasis, fluorescence-guided surgery, 5-ALA

About Oncotarget

Oncotarget is a bi-weekly, peer-reviewed, open access biomedical journal covering research on all aspects of oncology.

To learn more about Oncotarget, please visit https://www.oncotarget.com or connect with:

SoundCloud - https://soundcloud.com/oncotarget
Facebook - https://www.facebook.com/Oncotarget/
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Oncotarget is published by Impact Journals, LLC please visit https://www.ImpactJournals.com or connect with @ImpactJrnls

Media Contact
MEDIA@IMPACTJOURNALS.COM
18009220957

Top-Performer: White Light Versus 5-ALA Fluorescence Guided Resection

Handheld Raman Spectrometer

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Imaging/Microscopy

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