Imaging/Microscopy

Teach Me in 10

When analyzing complex compounds - such as PFAS, pesticides and mycotoxins - in challenging matrices with LC/MS/MS, labs face system contamination, matrix effects and time-consuming source cleaning. The combination of these can lead to inaccurate quantification and reduced sensitivity. Join Derek Mattern as he talks about ways that can help labs overcoming these challenges while meeting current regulations requirements.

Analyzing the Unanalyzable: Breakthrough in Complex Samples with LC/MS/MS

Biotech Videos 

Cysteine residues serve essential roles in protein structure and function due to their highly reactive thiol side chains that form inter or intra molecular di-sulfide bonds to enable correct protein folding.
https://www.creative-biolabs.com/adc/cysteine-based-conjugation.htm

Cysteine Based Conjugation

Aging (Aging-US)

Aging-US published this trending research paper on September 1, 2023, in Volume 15, Issue 17, entitled, “Development of a DNA damage-induced senescence model in osteoarthritic chondrocytes" by researchers from Nantes Université, Oniris, CHU Nantes, Inserm, Regenerative Medicine and Skeleton RMeS, UMR 1229, Nantes F-44000, France.

DOI - https://doi.org/10.18632/aging.204881

Corresponding author - Claire Vinatier - claire.vinatier@univ-nantes.fr

Abstract

Senescent cells (SnCs) have been described to accumulate in osteoarthritis (OA) joint tissues in response to injury, thereby participating in OA development and progression. However, clinical therapeutic approaches targeting SnCs using senolysis, although promising in preclinical OA models, have not yet proven their efficacy in patients with knee OA. This pitfall may be due to the lack of understanding of the mechanisms underlying chondrocyte senescence. Therefore, our study aimed to generate models of chondrocyte senescence.

This study used etoposide, to induce DNA damage-related senescence or chronic exposure to IL-1β to entail inflammation-related senescence in human OA chondrocytes. Several hallmarks of cellular senescence, such as cell cycle arrest, expression of cyclin-dependent kinase inhibitors, DNA damages, and senescence-associated secretory profile were evaluated.

Chronic exposure to IL-1β induces only partial expression of senescence markers and does not allow us to conclude on its ability to induce senescence in chondrocytes. On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components.

Etoposide-induced senescence model may help investigate the initiation of cellular senescence in chondrocytes, and provide a useful model to develop therapeutic approaches to target senescence in OA.

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Keywords - aging, senescence, osteoarthritis, etoposide, chondrocytes

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
X - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/@AgingJournal
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Development of DNA Damage-Induced Senescence Model in Osteoarthritic Chondrocytes | Aging-US

Custom Videos from Technology Networks

Manual sample preparation is labor-intensive, error prone, and requires highly skilled staff to
be done well. Increasingly, laboratories are turning to automation to reduce cost, improve reproducibility, and
minimize operator error.

This video explores several common myths surrounding laboratory automation technology. It also highlights compact, modular and flexible solutions that are ideal for both expert and novice users alike.

Watch this video from Agilent Technologies to discover the truth about lab automation.

Unravelling the Myths of Laboratory Automation

Shimadzu Analytical & Measuring

This video provides an overview of the iMScope QT imaging mass microscope, a robust tool for traditional mass spectrometry imaging experiments such as biomarker tracking, pharmacokinetics, and ADME studies as well as new applications. With its sub-cellular pixel resolution, high accuracy, ease of sample preparation, and user-friendly interface, the iMScope QT simplifies a previously arduous set of tasks into a routine workflow.  

Accelerate Throughput and Discovery for Your Mass Spec Imaging Projects

Aging-US published this trending research paper on April 18, 2023, in Volume 15, Issue 9, entitled, “Exogenous exposures shape genetic predisposition to lipids, Alzheimer’s, and coronary heart disease in the MLXIPL gene locus" by researchers from the Biodemography of Aging Research Unit, Social Science Research Institute, Duke University, Durham, NC; Department of Epidemiology, University of Pittsburgh, Pittsburgh, PA; Division of Statistical Genomics, Department of Genetics, Washington University School of Medicine, St Louis, MO.

DOI - https://doi.org/10.18632/aging.204665

Corresponding authors - Yury Loika - yury.loika@duke.edu, and Alexander M. Kulminski - alexander.kulminski@duke.edu

Abstract

Associations of single nucleotide polymorphisms (SNPs) of the MLXIPL lipid gene with Alzheimer’s (AD) and coronary heart disease (CHD) and potentially causal mediation effects of their risk factors, high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG), were examined in two samples of European ancestry from the US (22,712 individuals 587/2,608 AD/CHD cases) and the UK Biobank (UKB) (232,341 individuals; 809/15,269 AD/CHD cases). Our results suggest that these associations can be regulated by several biological mechanisms and shaped by exogenous exposures. Two patterns of associations (represented by rs17145750 and rs6967028) were identified. Minor alleles of rs17145750 and rs6967028 demonstrated primary (secondary) association with high TG (lower HDL-C) and high HDL-C (lower TG) levels, respectively. The primary association explained ~50% of the secondary one suggesting partly independent mechanisms of TG and HDL-C regulation. The magnitude of the association of rs17145750 with HDL-C was significantly higher in the US vs. UKB sample and likely related to differences in exogenous exposures in the two countries. rs17145750 demonstrated a significant detrimental indirect effect through TG on AD risk in the UKB only (βIE = 0.015, pIE = 1.9 × 10−3), which suggests protective effects of high TG levels against AD, likely shaped by exogenous exposures. Also, rs17145750 demonstrated significant protective indirect effects through TG and HDL-C in the associations with CHD in both samples. In contrast, rs6967028 demonstrated an adverse mediation effect through HDL-C on CHD risk in the US sample only (βIE = 0.019, pIE = 8.6 × 10−4). This trade-off suggests different roles of triglyceride mediated mechanisms in the pathogenesis of AD and CHD.

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Keywords - aging, MLXIPL, lipids, triglycerides, coronary heart disease, Alzheimer’s disease

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/@AgingJournal
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

External Factors Shape Genetic Predisposition to Lipids, Alzheimer’s and Heart Disease in MLXIPL Gene

With unparalleled speed, sensitivity and spatial resolution, Shimadzu’s iMScope QT imaging mass microscope clears the
way to next-generation mass spectrometry imaging. It enables fast acquisition of high-mass, high-resolution images at the optical and molecular level, can simultaneously visualize and analyze samples, and offers full fusion with morphology studies. Integration with the Q-TOF LCMS-9030 enables obtaining distribution information and quantitative data for comprehensive analysis and it's easy to switch between MS imaging configuration and LCMS.

High-End Imaging with an Unequalled Combination of Speed, Sensitivity, and Resolution 

TotalLab

In this video, I'll show you how you can obtain quantifiable data from lateral flow tests (LFT/LFA) in a 21 CFR Part 11/GxP/EU Annex 11 compliant manner using Phoretix 1D, which allows you to produce a full audit trail for every single lateral flow test you analyse to ensure regulatory compliance.

00:00 - Intro
00:20 - Explanation of TotalLab GxP Compliance Module
00:50 - How to log in to the software
01:40 - Username entry
01:50 - Password entry
02:17 - 21 CFR Sign in
02:23 - Project screen overview
02:32 - Creating a new compliant project
02:46 - Attaching notes to the audit trail of a project
03:10 - Adding files to your project
03:42 - Audit trail explanation
04:20 - Checking out a project for analysis
04:32 - Software integrations with the TotalLab GxP module
04:39 - Opening Phoretix 1D analysis software
04:50 - Analysing lateral flow tests
10:27 - Saving your analysis
10:32 - Audit trail build-up explanation
10:48 - Extended notes within the audit trail
11:40 - Checking a project back in to the secure folder
12:05 - Adding notes to a project check-in
12:23 - Checking the revision number of a project
12:54 - Viewing previous revisions of a project
13:08 - Viewing changes in data between revisions
13:41 - Exporting a 21 CFR compliant project report in .pdf
14:00 - Overview of report
15:20 - Sending a project for sign-off
15:50 - Adding notes for project sign-off to the audit trail
17:22 - Signing off a project
17:36 - Identifying projects awaiting sign-off
17:50 - Audit trail for project awaiting sign-off
18:36- Checking out a read-only copy of a project for inspection
19:20 - Password-protected sign-off
19:35 - Adding notes to project sign-off
21:05 - Project signed-off and closed

Quantifying the bands allows accurate comparison of the reactivity of different antibodies to positive samples and the definition of the limits of detection of those antibodies to positive samples (to determine the minimum testing sample volume).

A previous version of our 1D analysis software, CLIQS, has already been used for this purpose with the research published here: https://www.mdpi.com/2079-6374/11/12/510/htm

Another of our products, CLIQS 1D Pro, allows the building of a database of lateral flow tests that can be searched or allow tests to be compared to the entire database for similarities and differences.

For a free trial of Phoretix 1D to see how well it works with your lateral flow test images, please fill in the form here: https://totallab.com/products/phoretix-1d-free-trial/

For a free trial of CLIQS 1D Pro to see how well it works creating a database of your lateral flow test images, please fill in the form here: https://totallab.com/products/cliqs-1d-pro-free-trial/

For a free trial of our Phoretix 1D GxP software, to see how well it operates within a 21 CFR Part 11/GxP/Annex 11 compliant format, please fill in the form here:
https://totallab.com/products/gxp-module-demo/

#21CFR #GxP #lateralflowtest #LFA #analysis #GxP #database #software #LFT  #1D #imageanalysis #automatic #howto #antibody #testing

How to Analyse Lateral Flow Tests (LFT/LFA) in compliance with 21 CFR Part 11 Using Phoretix 1D

In this video, I'll show you how you can get free access to advanced Western blot/1D image quantification software.

00:00 - Intro
00:25 - Full free trial
00:40 - Free academic use licensing
01:10 - How to access free licensing mode
01:30 - Opening tutorial images
01:47 - Single-channel image analysis
03:03 - Image setup and contrast edits
03:45 - Automatic lane detection/manual lane detection
04:00 - Background removal
04:34 - Molecular weight marker import
05:10 - Multichannel image analysis

For a free trial of Phoretix 1D to see how well it works with your images, please fill in the form here:
https://totallab.com/products/phoretix-1d-free-trial/

Phoretix 1D can provide quantitative analysis of blot and gel images, lateral flow test images, TLC (thin layer chromotography) images and more.
 
See our other image analysis-based products here: https://totallab.com/products/

And if you want to get in touch with us here at TotalLab:

Website: http://www.totallab.com/ 
LinkedIn: https://www.linkedin.com/company/totallab-ltd/

#westernblot #electrophoresis #SDS-PAGE #tlc #quantification #immunoblot #software #imageanalysis #cliqs #totallab1d #imagej #21cfr #FDA #science #lifescience

Imaging/Microscopy

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