Imaging/Microscopy

Aging (Aging-US)

Aging (Aging-US) published this trending research paper in Volume 14, Issue 17, entitled, “Aging, prevalence and risk factors of MRI-visible enlarged perivascular spaces” by researchers from Boston University School of Medicine, Boston, MA, NHLBI’s Framingham Heart Study, Framingham, MA, Department of Biostatistics, Boston University School of Public Health, Boston, MA, Department of Radiology, Veterans Affairs Boston Health System, Boston, MA, Department of Neurology, Boston University School of Medicine, Boston, MA, The Glenn Biggs Institute for Alzheimer's and Neurodegenerative Diseases, University of Texas Health Sciences Center, San Antonio, TX, Department of Neurology, University of California at Davis, Davis, CA.

DOI - https://doi.org/10.18632/aging.204181

Corresponding author - Jose R. Romero - joromero@bu.edu 

Abstract

Background and purpose: Cerebral small vessel disease (CSVD) increases with age and is associated with stroke and cognitive decline. Enlarged Perivascular Spaces (ePVS) is an emerging marker of CSVD, but its prevalence over the life span remain unclear. We characterized the age and sex-specific prevalence of ePVS and relation to age-specific risk factors, in a large community-based sample.

Methods: We included 3,710 Framingham Heart Study participants with available brain MRI (average age 61.4±14.6, 46% men). ePVS burden was rated in the centrum semiovale (CSO) and basal ganglia (BG) regions. Individual vascular risk factors were related to ePVS burden in the CSO, BG, and mixed CSO-BG regions using multivariable adjusted ordinal logistic regression analysis.

Results: Severe ePVS prevalence increased with age in men and women, and paralleled increase in vascular risk factors, and prevention treatment use. Older age, hypertension (and resulting higher treatment use), higher systolic and diastolic blood pressure, and smoking were associated with higher burden of ePVS in the CSO, BG and mixed regions.

Conclusions: Our observations reinforce the hypothesis that ePVS may be a marker of aging-driven brain vascular pathologies, and its association with vascular risk factors support their role as CSVD imaging biomarker.

Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.204181

Keywords - cerebral small vessel disease, neurological markers, aging, disease marker, perivascular spaces

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website athttps://www.aging-us.com/​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Paper Spotlight: Aging, Prevalence and Risk Factors of MRI-Visible Enlarged Perivascular Spaces

Oncotarget

Oncotarget published this research paper on September 6, 2022 in Volume 13, entitled, "Targeted elastin-like polypeptide fusion protein for near-infrared imaging of human and canine urothelial carcinoma" by researchers from the Department of Chemistry, Purdue University, Bindley Bioscience Center, West Lafayette, IN; Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN; Department of Pathology and Laboratory Medicine, Indiana University, Indianapolis, IN; Department of Urology, Indiana University School of Medicine, Indianapolis, IN.

DOI - https://doi.org/10.18632/oncotarget.28271

Correspondence to - David Thompson - davethom@purdue.edu

Abstract

Cystoscopic visualization of bladder cancer is an essential method for initial bladder cancer detection and diagnosis, transurethral resection, and monitoring for recurrence. We sought to develop a new intravesical imaging agent that is more specific and sensitive using a polypeptide based NIR (near-infrared) probe designed to detect cells bearing epidermal growth factor receptors (EGFR) that are overexpressed in 80% of urothelial carcinoma (UC) cases. The NIR imaging agent consisted of an elastin like polypeptide (ELP) fused with epidermal growth factor (EGF) and conjugated to Cy5.5 to give Cy5.5-N24-EGF as a NIR contrast agent. In addition to evaluation in human cells and tissues, the agent was tested in canine cell lines and tissue samples with naturally occurring invasive UC. Flow cytometry and confocal microscopy were used to test cell-associated fluorescence of the probe in T24 human UC cells, and in K9TCC-SH (high EGFR expression) and K9TCC-Original (low EGF expression) canine cell lines. The probe specifically engages these cells through EGFR within 15 min of incubation and reached saturation within a clinically relevant 1 h timeframe. Furthermore, ex vivo studies with resected canine and human bladder tissues showed minimal signal from normal adjacent tissue and significant NIR fluorescence labeling of tumor tissue, in good agreement with our in vitro findings. Differential expression of EGFR ex vivo was revealed by our probe and confirmed by anti-EGFR immunohistochemical staining. Taken together, our data suggests Cy5.5-ELP-EGF is a NIR probe with improved sensitivity and selectivity towards BC that shows excellent potential for clinical translation.

Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.28271

Press release - https://www.oncotarget.com/news/pr/oncotarget-targeted-elastin-like-polypeptide-fusion-protein-for-near-infrared-imaging-of-human-and-canine-urothelial-carcinoma/

Keywords - bladder cancer, elastin-like polypeptide, NIR imaging, epidermal growth factor receptor (EGFR), translational studies

About Oncotarget

Oncotarget is a primarily oncology-focused, peer-reviewed, open access journal. Papers are published continuously within yearly volumes in their final and complete form, and then quickly released to Pubmed.

To learn more about Oncotarget, please visit https://www.oncotarget.com and connect with us:

SoundCloud - https://soundcloud.com/oncotarget
Facebook - https://www.facebook.com/Oncotarget/
Twitter - https://twitter.com/oncotarget
Instagram - https://www.instagram.com/oncotargetjrnl/
YouTube - https://www.youtube.com/OncotargetYouTube
LinkedIn - https://www.linkedin.com/company/oncotarget
Pinterest - https://www.pinterest.com/oncotarget/
Reddit - https://www.reddit.com/user/Oncotarget/

Media Contact
MEDIA@IMPACTJOURNALS.COM
18009220957

Trending With Impact: Imaging With Polypeptide Fusion Protein Targets Bladder Cancer

KRÜSS GmbH

Immerse yourself in the future of tensiometry and encounter Generation íí of our measuring technology at analytica in Munich, June 21-24, Booth A1.309.



More information:KRÜSS at analytica: https://www.kruss-scientific.com/de-DE/unternehmen/events/2022-06-analytica-germany
KRÜSS GmbH, Germany: http://www.kruss-scientific.com

Revealed at analytica | Discover Tensíío

Surface QC has never been that simple and reliable. Encounter Generation íí of our measuring technology at analytica in Munich, June 21-24, Booth A1.309.

More information:
KRÜSS at analytica: https://www.kruss-scientific.com/de-DE/unternehmen/events/2022-06-analytica-germany
KRÜSS GmbH, Germany: http://www.kruss-scientific.com

Revealed at analytica | Discover Ayríís

Revisit our booth at analytica in our event video and experience Generation íí of our instruments. Watch and listen to Dr. Andrew Mellor introducing Tensíío, our versatile flagship tensiometer, and Ayríís, the revolutionary contact angle instrument for quality control!

Generation íí revealed at analytica 2022 | Tensíío & Ayríís

Aging-US published this research paper on July 5, 2022 in Volume 14, Issue 14, entitled, "Common electrocardiogram measures are not associated with telomere length" by researchers Department of Cardiology, University Hospital, LMU Munich, Munich, Germany; German Centre for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany; Institute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany; Research Unit Molecular Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany; Institute for Medical Information Processing, Biometry and Epidemiology, Medical Faculty, LMU Munich, Munich, Germany; Institute of Genetic Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany; Institute of Medical Biostatistics, Epidemiology and Informatics (IMBEI), University Medical Center, Johannes Gutenberg University, Mainz, Germany; Pettenkofer School of Public Health Munich, Institute for Medical Information Processing, Biometry and Epidemiology, Medical Faculty, LMU Munich, Munich, Germany.

DOI - https://doi.org/10.18632/aging.204149

Corresponding author - Moritz F. Sinner - moritz.sinner@med.uni-muenchen.de

Abstract

Aims: Aging is accompanied by telomere shortening. Increased telomere shortening is considered a marker of premature aging. Cardiac aging results in the development of cardiac pathologies. Electrocardiogram (ECG) measures reflect cardiac excitation, conduction, and repolarization. ECG measures also prolong with aging and are associated with cardiac pathologies including atrial fibrillation. As premature prolongation of ECG measures is observed, we hypothesized that such prolongation may be associated with telomere length.

Methods and Results: We studied the large, community-based KORA F4 Study. Of 3,080 participants enrolled between 2006 and 2007 with detailed information on demographic, anthropometric, clinical, and ECG characteristics, 2,575 presented with available data on leukocyte telomere length. Telomere length was determined by real-time quantitative PCR and expressed relative to a single copy gene. We fitted multivariable adjusted linear regression models to associate the ECG measures RR-interval, PR-interval, QRS-duration, and heart rate corrected QTc with telomere length.

In our cohort, the mean age was 54.9±12.9 years and 46.6% were men. Increased age was associated with shorter telomere length (p<0.01), and men had shorter telomere length than women (p<0.05). In unadjusted models, heart rate (p=0.023), PR-interval (p<0.01), and QTc-interval (p<0.01) were significantly associated with shorter telomere length. However, no significant associations remained after accounting for age, sex, and covariates.

Conclusions: ECG measures are age-dependent, but not associated with shortened telomere length as a marker of biological aging. Further research is warranted to clarify if shortened telomeres are associated with clinical cardiac pathologies including atrial fibrillation.

Keywords - aging, electrocardiogram, telomere length, cardiac aging

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Paper Spotlight: Common ECG Measures are not Associated with Telomere Length

Teach Me in 10

Flow cytometry is a technique that is adopted to both detect and measure characteristics of a population of cells, or particles. As a widely used method, flow cytometry has several different applications. 
Watch this Teach Me in 10 video to hear an introduction to flow cytometry with Dr. Eric Yager from the Albany College of Pharmacy and Health Sciences.

An Introduction to Flow Cytometry With Dr. Eric Yager

ADVANCE is the innovative, workflow-oriented, and ever-growing software platform for a wide range of KRÜSS instruments. This video gives you an insight of basic feature as well as the benefits your gain with regular upgrades. With the Software Assurance license model for ADVANCE you can be sure never to miss an upgrade so that new features support you even more in your measurements and their evaluation. With the Software Assurance contract, the regular upgrades come in a much more cost-effective way compared to purchasing upgrades.

ADVANCE | Software Assurance

In this Teach Me In 10 episode we are joined by Michiel Fokkelman, head of high throughput screening at Crown Bioscience.
We will discuss the concept and definitions of high content imaging, both for 2D and 3D cell culture systems. These rich and extensive morphological readouts provide phenotypic data showing the cellular response(s) to a compound or new potential drug. Michiel then explores the use of 3D-cultured organoids and how these are utilized in drug discovery and pre-clinical drug development using high content approaches. Finally, we will then look at the role of lab automation, essential in enabling high throughput and HCI-based phenotypic screening, and how you can benefit from these technologies.

Imaging/Microscopy

Refine By

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.