Genomics

CD Genomics

As a leading provider of NGS services, CD Genomics is providing an integrated portfolio of methylation sequencing services. Whole genome bisulfite sequencing (WGBS) is an effective and reliable strategy to identify individually methylated cytosines on a genome-wide scale. With over 10 years of experience and the state-of-the-art next-generation sequencing platforms, we can totally meet your project requirements and budgets in the exploration of methylome. 


https://www.cd-genomics.com/Whole-Genome-Bisulfite-Sequencing.html

Principle and Workflow of Whole Genome Bisulfite Sequencing

RNA-Seq is the premier tool for mapping and quantifying transcriptomes by utilizing next-generation sequencing (NGS) technology. The transcriptome refers to the complete set of transcripts in a cell, which provides information on the transcript level for a specific developmental stage or physiological condition. Understanding the transcriptome is necessary for interpreting the functional elements of the genome and understanding development and disease. The key purpose of transcriptomics includes cataloging all species of transcripts; determining the transcriptional structure of genes; and quantifying the expression levels of each transcript under different conditions.

The Development and Workflow of RNA-seq, Learn more at https://www.cd-genomics.com/resourse-Bioinformatics-Workflow-of-RNA-Seq.html

The Development and Workflow of RNA-seq

Oncotarget

Abstract:
Melanoma is the deadliest form of skin cancer. Despite recent advances in medicine and the development of new treatments for melanoma, cures remain elusive as acquired resistance to both targeted and immunotherapies are becoming common. Therefore, more studies are conducted to dissect underlying molecular mechanisms that drive melanoma growth in order to provide better therapeutic option. Here, employing a comprehensive and unbiased analysis of different RNA modification regulatory proteins using various publicly available databases we identify the most relevant RNA modifying proteins that plays crucial role in melanoma development. Our study started with the analysis of various genetic alterations (amplifications, mutations/deletion) as well as RNA overexpression of these RNA modification regulatory proteins in The Cancer Genome Atlas melanoma database. We then analyzed their expression in The Human Protein Atlas data. The result of analysis revealed that only a subset of RNA modification regulatory proteins are overexpressed in >75% of melanoma patient cases as compared to normal skin. However, when examined in Oncomine dataset we found only two genes (METTL4 and DNMT3A) were significantly overexpressed in melanoma samples versus normal skin samples and matched with the results of The Human Protein Atlas data. Therefore, we functionally validated METTL4 and DNMT3A using shRNA-mediated knockdown and found that their knockdown in melanoma cells led to melanoma cells growth inhibition. Collectively, in this study, we investigated the epitranscriptomic landscape of melanoma using various publicly available database and identified DNMT3A and METTL4 as the most relevant potential regulators of melanoma growth.

Oncotarget is a bi-weekly, peer-reviewed, open access biomedical journal covering research on all aspects of oncology.

To learn more about Oncotarget, please visit https://www.oncotarget.com or connect with:

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Facebook - https://www.facebook.com/Oncotarget/
Twitter - https://twitter.com/oncotarget
YouTube - https://www.youtube.com/c/OncotargetYouTube/
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Reddit - https://www.reddit.com/user/Oncotarget/

Oncotarget is published by Impact Journals, LLC please visit https://www.ImpactJournals.com or connect with @ImpactJrnls

Media Contact
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Trending with Impact: The Role of RNA Modification Regulatory Proteins in Melanoma

Teach Me in 10

This week at Teach Me in 10, we are joined by Oded Rechavi, a professor in molecular biology at Tel Aviv University in Israel. The tagline for the Rechavi lab is "The Laboratory for Radical Science", and in this ten-minute talk, you will learn why. Rechavi's research spans various different topics, including the study of novel mechanisms of inheritance and applying genomics analyses to the ancient Dead Sea Scrolls. To learn more about the Rechavi laboratory, you can visit the website, or read the profile piece Technology Networks published in 2020.

The Laboratory for Radical Science With Professor Oded Rechavi

Cytiva

Our Lyo-Stable stabilization service locks in performance, enabling room temperature shipping and storage. Our patented lyophilization technology stabilizes individual proteins, reagents and complete multiplex assays, by providing a molecular environment that protects against conformational changes in protein structure.

Lyo-Stable lyophilization services

Cytiva's custom conjugation service optimizes reagents and magnetic beads using concentration, reformulation, binding of enzymes, antibodies and custom ligands and adapting particle sizes and functionalities.

Reagent and magnetic bead conjugation services

Our customized solutions and stabilization services simplify and streamline your genomics, molecular biology and immunodiagnostics workflows. Whether creating a custom bead, a specialized enzyme, or a convenient kit for a repeated workflow, we will work with you to share progress and optimize outcomes. Our team of scientists and manufacturing specialists is available to provide custom solutions including lyophilization, conjugation, custom biology, custom plates and contract manufacturing that fit seamlessly into your existing protocols, often saving you time and cost.

Customize solutions for genomics and diagnostics

CD Genomics is committed to providing novel NGS services enabling researchers to explore structure and function of the microbial community in a high-resolution and culture-independent manner using technology from Illumina and PacBio. In addition, we also offer the service to sequence the genome of the individual cultured bacteria, fungi, phage or virus, whether do novo or re-sequencing.  https://www.cd-genomics.com/Microbial-Genomics.html

Microbial Genomics — CD Genomics

Learn more about Microsatellite Genotyping at CD Genomics: https://www.cd-genomics.com/

Genomics

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