Flow Chemistry

Creative Enzymes

Creative Enzymes provides custom services for expression and production of both natural and recombinant enzymes in various systems. Creative Enzymes established and developed expression systems for all types of enzymes, covering early stage of cDNA preparation, DNA cloning, enzyme expression, all the way to production scale-up. Our outstanding scientists and strategic partners professionally handle every detail of each step.   https://www.creative-enzymes.com/service/Enzyme-Expression-And-Production_225.html

Enzyme Expression and Production - Creative Enzymes

Alfa Chemistry

Alfa Chemistry offers a powerful range of carbon nanotube nanomaterials, including:

Carbon Nanotubes
Purified Single-wall Carbon Nanotubes
Carbon Nanotube Sponges
Oriented Carbon Nanotube Array

Learn more at https://www.alfa-chemistry.org/products/carbon-nanotube-nanomaterials-45.htm

Carbon Nanotube Nanomaterials

SiliCycle Inc.

The relationship between retention factors and column volumes is important to transfer from TLC to flash chromatography.

Have more questions? Head over to our FAQs:
https://www.silicycle.com/faq

Learn more about SiliaSep:
https://www.silicycle.com/products/siliasep-flash-cartridges

Learn more about SiliaPlate:
https://www.silicycle.com/products/thin-layer-chromatography-tlc-plates

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FAQ What is the relationship between retention factor and column volume?

The basis of smoothly going from TLC plates to flash chromatography starts with the retention factor (Rf).  

Inaccurate Rf’s will lead to erroneous column volumes (CVs) and then completely skew all the method development. 

Have more questions? Visit our TLC FAQ guide: https://www.silicycle.com/faq/thin-layer-chromatography

Get TLC best practices with our blog article:
https://www.silicycle.com/articles/cat/product-blog/post/thin-layer-chromatography-plates-use-and-best-practices/

Learn more about SiliaPlate:
https://www.silicycle.com/products/th...


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Brighter Days by Markvard & Kvarmez 
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FAQ How to calculate retention factor (Rf)?

Don't be fooled,  column volume does not mean the internal volume of  the column without the packing material inside!
Also known as CV, column volumes are an important part of column chromatography as they help determine purification run length, gradient profile, and solvent consumption.  

Get an more in-depth overview of column chromatography with our free Application Note:
https://www.silicycle.com/resource-center/application-notes/quick-overview-of-column-chromatography

Have more questions? Visit our FAQ guide: 
https://www.silicycle.com/ca/faq

Learn more about SiliaSep Flash Cartridges: https://www.silicycle.com/products/siliasep-flash-cartridges


Music Credit: 
Brighter Days by Markvard & Kvarmez 
https://soundcloud.com/markvard
https://soundcloud.com/simen-eliassen...
Creative Commons — Attribution 3.0 Unported  — CC BY 3.0 
Free Download / Stream: https://bit.ly/brighter-days
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FAQ Understanding Column Volume

Chromatography & Mass Spectrometry Solutions

pH is a critical parameter in method development and routine enzyme assay measurement.  At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable determination of the optimal pH is important for successful enzyme assay method development. 
The Thermo Scientific Gallery Enzyme Master Enzyme Analyzer, with the optional electrochemistry measurement (ECM) unit, allows the parallel determination of sample pH and conductivity.  The Gallery Enzyme Master system can measure up to 67 samples for pH and conductivity in an hour alongside photometric enzyme assay analysis. Parallel determination of optimal pH makes the overall enzyme assay analysis easier, faster, and reliable.

Fully-Automated Enzyme Optimal pH Analysis

Get more from your enzyme method development process—starting with walkaway efficiency. The Thermo Scientific™ Gallery™ Enzyme Master enzyme analyzers are the first automated enzyme analyzers designed specifically for enzyme assay applications. Combining robust hardware and new custom designed software, these best-in-class solutions deliver fully-automated incubation settings, reagent additions, and precise measurement calculations—all with a touch of a button. They offer the smartest (and fastest) way yet to streamline method development and deliver reliable results.

Fully-automated enzyme assay analysis

Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Cytochrome c reductase is a flavoprotein that completes the oxidation-reduction chain between hexosemonophosphate and cytochrome c.  More can be reached at Creative Enzymes: https://www.creative-enzymes.com/

Enzymatic Assay of Cytochrome c Reductase

In biochemistry, the substrate is a molecule upon which an enzyme acts. Enzymes catalyze chemical reactions involving the substrate(s). In the case of a single substrate, the substrate bonds with the enzyme active site, and an enzyme-substrate complex is formed. The substrate is transformed into one or more products, which are then released from the active site. The active site is then free to accept another substrate molecule. In the case of more than one substrate, these may bind in a particular order to the active site, before reacting together to produce products.  https://www.creative-enzymes.com/service/Screening-Of-Substrates-Inhibitors-And-Other-Ligands_65.html

Flow Chemistry

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