Flow Chemistry

Teach Me in 10

In this episode of Teach Me in 10, Lucy Lawrence is joined by the Global Product Manager of Applications and Flow Reagents at Bio-Rad Laboratories, Dr. Chris Brampton.

Chris will cover the basics of antibodies, the reasons why you need flow cytometry–specific antibodies and some basic best practices to achieve improved data and increase the likelihood of a successful outcome for flow cytometry experiments.

Top Tips for Antibodies and Flow Cytometry With Dr. Chris Brampton

SiliCycle Inc.

E-PAK is a family of radial flow purification cartridges that linearly scale-up from lab to commercial processing sizes, which means that lab conditions and results are easily reproducible. 

They are four ways/ configurations that one can use E-PAK cartridges:
-Single Pass
-Recirculation
-Multi pass
-Series pass

Watch the video to see each one in action. 

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Also be sure to check out our Application Notes library to see industry examples of when and how to use E-PAK:  
https://www.silicycle.com/resource-center/application-notes


Methods of using E-PAK

Creative Enzymes

Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme's kinetics can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or an agonist might inhibit the enzyme. Creative Enzymes is a renowned service provider, supporting many industrial and academic researchers for kinetic studies. https://www.creative-enzymes.com/service/Enzyme-Kinetics_393.html

Enzyme Kinetics at Creative Enzymes

E-PAK is a family of radial flow purification cartridges developed specifically for pharmaceutical processing from the lab scale all the way up to commercial sizes. 

Scaling up is easy when  it comes to E-PAK as it follows a linear trend, that way you can be sure that your lab results can be easily achieved at pilot and commercial purification steps.    

Offered with a choice of metal scavengers, bare silica or activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Have questions about metal scavengers? Visit our FAQ page: https://www.silicycle.com/faq/metal-organic-scavengers

SiliCycle also has a product blog page and frequently touches on the drug purification. Some articles that may be of interest include;
Excelling at scaling-up: https://www.silicycle.com/articles/cat/product-blog/post/some-people-excel-at-scaling-up-their-purifications-and-some-dont-which-one-are-you
Streamlining scale-up: https://www.silicycle.com/articles/cat/product-blog/post/how-to-streamline-metal-scavenger-scale-up-during-drug-development/
Simplifying catalyst removal: https://www.silicycle.com/articles/cat/product-blog/post/simplifying-metal-catalyst-removal-in-drug-development-applications/
Eliminating metal contamination: https://www.silicycle.com/articles/cat/product-blog/post/pharmaceutical-metal-scavengers/


E-PAK cartridge housings, from lab to commercial scale

Created with proprietary technology, E-PAK radial flow cartridges provide rapid adsorption kinetics and processing capacities for laboratory, pilot and commercial operations. They are designed for use with both organic and aqueous solvents, and incorporate design features useful for the production of active pharmaceutical ingredients (API).

Features include:
• Proven cartridge design to ensure rapid, simple & reliable technology
• Fixed-bed design ensuring safer handling, clean-up and disposal
• Large adsorbent capacity in small area footprint increases product recovery & reduces solvent requirements

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Take a closer look at the E-PAK cartridge

E-PAK is a family of radial flow purification cartridges developed specifically for pharmaceutical processing.

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Have questions about metal scavengers? Visit our FAQ page: https://www.silicycle.com/faq/metal-organic-scavengers

Also check out our Application Notes library to see some examples of when  and how to use E-PAK cartridges to help with purification needs:   https://www.silicycle.com/resource-center/application-notes


What are E-PAK radial flow cartridges?

Feiyuebio Antibodies - ELISA - Protein

Your Bulk Order
96 wells plate
Research used ELISA
Science & Technology Solutions In Life Science.
Feiyuebio provides a wide range of ready-to-use ELISA




Feiyuebio Research used ELISA

Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Cytochrome c reductase is a flavoprotein that completes the oxidation-reduction chain between hexosemonophosphate and cytochrome c.  More can be reached at Creative Enzymes: https://www.creative-enzymes.com/

Enzymatic Assay of Cytochrome c Reductase

Flow cytometry can generate exquisitely detailed information about individual cells at a resolution comparable to single-cell technology. 

In this Teach me in 10, we are joined by Richard Cuthbert, global commercialization product manager at Bio-Rad Laboratories. We discuss some of the challenges associated with high-throughput screening technologies and the benefits of flow cytometry. We also debate how recent advancements in the field have vastly increased the throughput capabilities which can be further enhanced by integrating robotic work cells, making it a viable prospect as a screening technology.

We also highlight important factors to consider when purchasing a flow cytometry platform for screening applications. Finally, we explore the current unmet needs in the field, speculate on future advancements and suggest some useful resources to help you learn more about flow cytometry.

Flow Chemistry

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