Biopharma

Teach Me in 10

In some patients, the rapid antidepressant action of ketamine can last for days, or even weeks. This, says Dr. Lisa Monteggia, is incredible for a drug that is cleared from the body very quickly. Over recent years, The Monteggia laboratory at the University of Vanderbilt has been investigating the molecular mechanisms that underly this rapid action.  In this Teach Me in 10, Dr. Monteggia outlines some of the key discoveries that her team have made, particularly in relation to ketamine, neuroplasticity and neuroreceptors.

Ketamine as an Antidepressant With Dr. Lisa Monteggia

Mutant library

Creative Biogene provides world-class packaging services for a variety of viral types using our QVirusTM platform. Creative Biogene's state-of-the-art facilities and highly experienced staff are available in order to assist in all areas of virus vector design and construction, as well as the generation of the virus in a quick turnaround. Our custom viral services are highlighted by a consistent high titer and large delivery viral counts.

https://www.creative-biogene.com/Services/Custom-Viral-Service

Custom Viral Service

IntegrateRNA is a leading provider in the field of RNA biology and focused on provide high-quality and customized products and services to accelerate your vaccines development. We have experienced experts and advanced platforms that are able to solve the problems in basic research and clinical trials. If you are interested in our services, please contact us for more details.

https://integraterna.creative-biogene.com/application/mrna-vaccines.html

mRNA Vaccine

Creative Enzymes

Creative Enzymes is a worldwide leading company in diagnostic enzymes manufacturing and supply. We are committed to providing our customers with diverse enzyme products and custom enzyme-related services for medical and research diagnosis. Relying on our professional team and state-of-art technologies, we have gained solid reputation for having top of the line products and services.

Our product lines cover a wide range of application areas, including but not limited to blood lipids, liver function, kidney and pancreas function, diabetes, POCT bio-sensor and clinical diagnosis. Every product is presented with detailed description, source, form, activity and other biochemical properties. Our high-quality products always ensure the rapid, reliable and reproducible experiments.  https://diagnostic-enzymes.creative-enzymes.com/

Diagnostic Enzymes - Creative Enzymes

Sekisui XenoTech

Presenter: Dr. Brian Harvey, Emeritus Professor of Molecular Medicine at the Royal College of Surgeons in Ireland

Key concepts discussed in this webinar will include:
 - Background to the clinical use of dexamethasone in treating severe COVID-19
 - Novel molecular targets of dexamethasone in treating pulmonary edema in COVID-19
 - Molecular mechanisms of inhibitory effects of dexamethasone on ion channels
 - Gender disparity in inflammatory lung disease: COVID-19, Asthma and Cystic Fibrosis
 - Molecular mechanisms of sexual dimorphism in COVID-19 and CF
 - Sex hormone-related targets in COVID-19 and CF

Abstract:
In this webinar we will discuss novel molecular targets of the steroid dexamethasone and the steroid hormone estrogen in airway disease with a focus on Covid-19, asthma and cystic fibrosis.

Dexamethasone is a synthetic steroid commonly used in the treatment of inflammatory airway disease which has found a repurposed use in the clinical management of severe COVID-19 lung disease with great success in reducing mortality. The few clinical studies ascribe the therapeutic effects of dexamethasone to its anti-inflammatory actions, however, this does not explain the steroid action on reducing pulmonary edema per se and other targets may be involved. We have recently shown effects of dexamethasone to reduce secretion across human airway cell lines by inhibiting ion channels and these novel effects of the steroid will be discussed in detail.

A gender disparity has been observed since the first reports of COVID-19 appeared with more men than women suffering severe COVID19 disease and dying from it. The molecular mechanisms for this sexual dimorphism are still unknown although the evidence points to differences in estrogen hormone levels and estrogen receptor expression and their regulation of ACE2 receptor biology, after taking into account patients age and underlying comorbidities. We know that a ‘gender gap’ also exists in other lung diseases such as asthma and cystic fibrosis. Asthma has a higher prevalence in boys than in girls before puberty and a higher prevalence and severity in women than in men in adulthood. A similar trend is seen in cystic fibrosis with girls having an earlier onset of lung disease at puberty with more frequent pulmonary exacerbations and shorter life span than men in adulthood. Because of the complexity of these diseases, no single straightforward mechanism can explain the gender differences found in asthma and cystic fibrosis. In the latter half of the webinar we will focus on the influence of female sex hormones on CF pulmonary outcomes and summarise data from in vitro and in vivo experiments on how estrogen might modify airway clearance, immunity and bacterial infection in the CF airways. The potential for novel steroid hormone-related therapeutic interventions will also be discussed.

Download a copy of the slides: https://www.xenotech.com/wp-content/uploads/2021/11/Ion-Channel-Targets-of-Dexamethasone-and-Sex-Steroid-Hormones-in-Airway-Disease.pdf
https://www.xenotech.com/about/events/webinar-role-of-udp-glucuronosyltransferases-in-drug-metabolism-and-drug-drug-interactions/
Questions, comments and requests: https://www.xenotech.com/contact/
More information on our research services and test systems: https://www.xenotech.com
Other previously aired webinars: https://www.xenotech.com/access-adme-research-resources/resource-type/webinar/
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Request a webinar topic: https://www.xenotech.com/contact/webinar-seminar-request/

About the Presenter:
Dr. Brian Harvey is Emeritus Professor of Molecular Medicine at the Royal College of Surgeons in Ireland. He is also a Principal Investigator in the Ion Channel Group at the Centro di Estudios Cientificos, Chile, and Visiting Research Professor at the Sorbonne University, Paris. Brian holds a PhD in Physiology and Biophysics from the National University of Ireland and a DSc honoris causa from Michigan State University. His main research area is in cell and molecular endocrinology of rapid responses to steroid hormones and their regulation of ion channels on which he has published over 300 papers. He is a Chevalier of the French Order of Merit and Member of the Ordre des Palmes Academiques, and an elected fellow of the European Academy of Sciences and the Royal Irish Academy.

COVID-19 Drug Repurposing Webinar: Ion Channel Targets of Dexamethasone and Sex Steroid Hormones in Airway Disease

Oncotarget

Oncotarget published "Benzophenone-3 promotion of mammary tumorigenesis is diet-dependent" which reported that Benzophenone-3 is a putative endocrine disrupting chemical and common ingredient in sunscreens.

Although benzophenone-3 seemed protective on low-fat diet, spindle cell tumors arising in these mice showed increased proliferation and decreased apoptosis. This points to a need for further studies of benzophenone-3 in both animal models and humans as a potential breast cancer risk factor, as well as a more general need to evaluate endocrine disrupting chemicals in varying dietary contexts.

Dr. Richard C. Schwartz and Dr. Sandra Z. Haslam, both from The Michigan State University said, "Ovarian hormones are strongly implicated in the etiology of breast cancer."

Putative endocrine disrupting chemicals, particularly estrogenic chemicals, have emerged as suspects in environmental promotion of breast cancer. Environmental EDCs have the potential to act as agonists or antagonists in critical hormonally regulated processes, such as mammary gland development and mammary tumorigenesis - this warrants evaluation of their potential in promoting breast cancer.

More recently, BP-3 was demonstrated to have pathological effects on coral. Although BP-3 has a very short half-life, its presence is widespread in human urine, in as much as 98% of the general U.S. population.

A recent preliminary study found plasma concentrations greater than 0.5 ng/mL among a small human cohort using heavy topical applications of commercial sunscreens. The present study examined the interaction of BP-3 with HFD on mammary tumorigenesis in BALB/c mice, using the Trp53-null transplantation model. A level of BP-3 exposure was used that yielded levels in murine urine similar to that observed in humans subjected to heavy topical exposure of BP-3-containing commercial sunscreen. These authors found that BP-3 had complex effects that were dependent upon dietary regimen and tumor histopathology.

The Schwartz/Haslam Research Team concluded in their Oncotarget Research Output that unlike several earlier studies that found rather minimal BP-3 activity in vivo in rodents with doses higher than those in the current study: 1500 mg/kg BW/d; 1000 mg/kg BW/d; 150 mg/kg BW/d, these authors tumorigenesis experiments showed significant effects at 70 mg/kg BW/d. This dosage elicits levels of BP-3 excretion in urine similar to that observed in humans with heavy topical application of BP-3-containing sunscreen. Furthermore, their initial experiments in wild type BALB/c mice found significant effects on mammary epithelial proliferation at only 7 mg/kg BW/d. These observations suggest caution in the use of BP-3-containing sunscreens.

DOI - https://doi.org/10.18632/oncotarget.2...

Full text - https://www.oncotarget.com/article/27...

Correspondence to - Richard C. Schwartz - schwart9@msu.edu and Sandra Z. Haslam - shaslam@msu.edu

Keywords - oxybenzone, benzophenone-3, mammary tumorigenesis, dietary animal fat, breast cancer

About Oncotarget

Oncotarget is a biweekly, peer-reviewed, open access biomedical journal covering research on all aspects of oncology.

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Author Insight: Benzophenone-3 Promotion of Mammary Tumorigenesis is Diet-dependent

Creative Bioarray

The induced pluripotent stem (iPS) cells from patient's somatic cells could be a useful source for drug discovery and cell transplantation therapies. To date, most iPS cells were made by using viral vectors, such as retroviruses and lentiviruses. Here we describe a stepwise protocol for the generation of modified virus-derived iPS cells from primary human fibroblasts.

https://www.creative-bioarray.com/support/reprogramming-of-human-fibroblasts-into-ipscs.htm

Differentiation of iPSCs

With the world's most comprehensive list of research-use cells, Creative Bioarray offers you a wide variety of cell-based assays for greater accuracy and increased throughput. We provide fully quantitative results for every one of cell-based assays.

https://www.creative-bioarray.com/Services/cell-line-based-services.htm

Cell Cycle Regulation

Protein kinases represent one of the most promising groups of drug targets owing to their involvement in such pathological conditions such as cancer, neural disorders, inflammatory diseases, and metabolism problems. Classical methods to analyze kinase activity involve the quantification of phosphoryl transfer by detecting the production of the phosphorylated product or the changes in the ratio of ATP to ADP. To assist the efforts in kinase drug discovery, Creative Bioarray offers high quality, time efficient and cost effective kinase solution for assay development, high throughput screening (HTS) and selectivity profiling.

https://dda.creative-bioarray.com/kinase-assay.html

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