Biopharma

Octet® Bio-Layer Interferometry (BLI) Platform

The Octet® RH16 and RH96 systems are the benchmark for high throughput monitoring of real-time binding of proteins and other biomolecules. Learn more at: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection 

The Octet® platform will improve the overall drug discovery process to increase the success rate of preclinical candidates and identify elite candidates earlier in screening. 16-channel Octet® RH16 is ideal for high-throughput applications that demand high sensitivity and low sample volume requirements.  The 96-channel ultra-high-throughput Octet® RH96 monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed.
From IgG titer to epitope binning, the Octet® platform offers label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
00:01 The Sartorius Octet® allows researchers to analyze protein-protein and protein-small molecule interactions and represents the industry-standard for label-free analyses.
00:13 The Octet® RH16 and RH96 systems are the benchmark for high throughput monitoring of real-time binding of proteins and other biomolecules.
00:26 With the ability to read up to 96 samples in parallel, titer determinations and kinetic screens are completed in just minutes, rather than hours.
00:35 High detection sensitivity makes these systems favorites for small molecule library and fragment screening, as well as analysis of large cross-competition binding matrices.
00:48 Target ligands can be individually, or batch immobilized onto biosensors, which can be regenerated and placed back into the biosensor tray for re-use in future experiments. 
01:01 Key applications benefitting from the unique strengths of the Octet® RH systems include off-rate ranking for clone selection, titer determination and antibody competition. 
01:14 The RH96  allows complete titer determinations for a 96-well plate of IgGs in just two minutes or provides 384 binding kinetics results in as little as 15 minutes.
01:28 You can also perform customized, multi-step quantification assays for applications such as host cell protein contaminations and residual protein A detection, allowing detection of sub-nanogram per milliliter concentrations.
01:45 These systems are also ideally suited to epitope binning experiments.  For example, with the Octet® RH96, a 32 by 32 matrix can be completed in record time, with a dedicated epitope binning analysis function to enable easy visualization of data sets. The software also provides a flexible data matrix that can accommodate a variety of cross-blocking formats.
02:12 You can further increase productivity by using the systems’ high throughput capabilities to perform multiple assays in the same sample. For example, you can perform glycan screening and analyte titer determinations as early as the subcloning stage, helping to ensure only high-quality candidates continue downstream, and preventing late failures.
02:37 In summary, the Octet® RH16 and RH96 systems accelerate discovery, process development and manufacturing for biologics and vaccines, so more people will have access to better medicines, faster.
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 #BioLayerInterferometry #BLI #Octet #GenuineOctet



Octet® RH16 and RH96 | High Throughput Monitoring of Binding of Proteins and Other Biomolecules

Label-Free High-Quality Kinetics and Quantitation in Real-Time - Octet®️ Bio-Layer Interferometry (BLI) systems offer an advanced, fast, robust, and fluidics-free approach for protein-protein and protein-small molecule analysis. Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection
The Octet®️ platform is comprised of instruments, software, biosensors, reagents and assay kits for biomolecular interactions analysis in standard microplates. All Octet®️ instruments have comparable sensitivity and direct detection of specific proteins or drug molecules - even in complex mixtures and unpurified samples such as cell culture supernatants and lysates. The Octet®️ platform is a scalable solution that can process up to 96 samples in parallel. This unprecedented throughput and flexibility will empower scientists by enabling faster time to market, quicker project completion, and greater project capacity.

00:02 The Sartorius Octet® allows researchers to analyze protein-protein and protein-small molecule interactions and represents the fluidic free industry standard for label-free analysis.
00:14 The Bio-Layer Interferometry platform includes instruments biosensors reagents assay kits and software to measure binding affinities kinetics and concentrations in real time.
00:27 This integrated solution makes it significantly faster and easier to perform robust characterization of biomolecules and drug candidates accelerating workflows and delivering stress-free data for early decision making.
00:42 Sartorius's latest offering the Octet® R series provides a modular and scalable solution for molecular screening in 96 well microplates.
00:51 Offering a choice of two four or eight channel parallel processing, these instruments are designed to provide fully automated walk-away processing to meet every lab's budget and throughput requirements.
01:06 The entry-level octet r2 is intended for labs with low throughput requirements or a limited budget.
01:12 It brings the benefits of bio-layer interferometry to a wider audience, including features such as robust temperature control and microplate cooling that were previously only available on larger high-end systems.
01:26 The modular design of the r series makes it possible to upgrade your instrument as your throughput requirements change.
01:35 For example an Octet® R2 system can be converted to an Octet® R4 in the field, doubling your throughput instantly and offering the perfect balance between performance and price.
01:46 You can even upgrade to an Octet® R8 to further increase throughput without the instrument ever leaving your lab.
01:53 This helps to protect your investment and future proof your workflows without the need to redesign assays and protocols as throughput increases.
02:04 the top of the range Octet® R8 offers high throughput analysis while still being within budget of most labs.
02:11 Intended for use in both pure research and GMP environments the Octet® R8 is designed to work seamlessly with 21 CFR part 11 compliance software simplifying and streamlining the compilation of documentation for regulatory filings.
02:26 This system also features advanced evaporation protection allowing long runtime experiments and high affinity binding kinetic studies to be performed without compromising on data quality.
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Octet®️ Platform: Instrument Showcase

Adeno-associated virus (AAV), with low immunogenicity and toxicity, has become one of the most promising viral vectors used for delivery of genetic material in gene therapy. AAV vector development and production at industrial scale requires an efficient method for accurate quantitation of viral capsids in both upstream and downstream bioprocessing. 

Octet® Bio-Layer Interferometry (BLI) systems in combination with the Octet® AAVX Biosensors offer a rapid, label-free, and high-throughput method for quantification of AAV capsids. This video compares the Octet® AAVX biosensor workflow to that of ELISA at various stages in bioprocess development.

Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection/biosensors-and-kits/octet-aavx-biosensors

00:04 Meet Edward and Anne, scientists working in process development for a biopharmaceutical company developing gene therapies. Both are involved in the development of adeno-associated viral vectors, or AAVs. Ed works in upstream development, while Anne works on the downstream recovery and purification of biosynthetic products. 

00:25 AAV capsid titer is a critical quality attribute that must be monitored throughout process development and the production of AAVs, but Ed and Anne take two very different approaches.
00:38 Ed uses an ELISA, which requires six steps and takes three to four hours to perform. Unfortunately, Ed’s latest ELISA run delivered inconsistent results, so he’s going to need to stay late, again, and redo the ELISA.

00:55 In contrast, Anne uses the Octet® Bio-Layer Interferometry platform in combination with the Octet® AAVX Biosensors.  

01:04 The AAVX biosensor quantitation assay consists of a single step, and AAV titers can be measured in as little as 15 minutes, enabling near real-time decision making.

01:17 Anne leaves work at her usual time and enjoys the evening with friends and family.

01:24 Octet® AAVX Biosensors provide a rapid, direct and high throughput approach for the quantitation of AAV capsids in both crude and purified samples. The biosensors offer high precision and allow for quantitation of 10 different serotypes from AAV1 to AAV9 and AVVrh10.

01:47 Octet® BLI systems are fluidic-free and label-free, enabling non-destructive sample analysis. Anne uses the Octet RH96 system, which can analyze up to 96 samples simultaneously.

02:02 The next day, Ed and Anne meet at the water cooler. Ed mentions how he spent the evening re-running ELISAs and missed his daughter’s soccer game. Anne offers to share her secret for high throughput AAV capsid titers and provide a demonstration of the Octet® BLI platform. Ed states that he’s available now if she has the time. “No problem,” says Anne, and they walk to her lab.

02:28 For over 15 years, the industry-proven Octet® Bio-Layer Interferometry platform has pioneered real-time, robust, label-free analysis for the determination of affinity, kinetics, and analyte quantitation.

02:43 Explore Octet® AAVX Biosensors and systems by visiting our website.

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#AAV #virus #ELISA


Rapid Quantitation of AAV Capsids in Bioprocess Development for Gene Therapy Using Octet®️ BLI

Label-Free High-Quality Kinetics and Quantitation in Real-Time - The Octet®️ Bio-Layer Interferometry (BLI) platform is a comprehensive solution for screening and characterizing molecular interactions such as protein-protein or protein-drug interactions. Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection 

It enables an enviable variety of applications throughout the drug discovery and development process — from early selection to validation to manufacturing. Also, BLI-based analysis is widely used in biopharma and academic research. Octet®️ BLI label-free molecular interaction analysis provides critical information for specificity and affinity of biomolecular interactions for monitoring concentration and Critical Quality Attributes (CQA) in basic research, drug discovery, and bio-therapeutics manufacturing. Visit our BLI applications pages: https://www.sartorius.com/en/applications/life-science-research/label-free-detection

00:02 Bio-layer interferometry has applications throughout biomolecular analysis workflows and the drug discovery pipeline. 

00:11 The Octet®️ range of BLI instruments offers benefits across the entire workflow, irrespective of the number of samples which need to be analyzed.

00:20 In the early phase of target ID and validation, the Octet®️ Bio-Layer Interferometry can be used to determine the nature of molecular interactions with your target molecule or receptor, helping to establish the mechanism of action for novel therapeutic candidates.

00:35 Affinity testing can then be used in candidate screening, using on- and off rate binding data to support lead selection. 

00:43 The Octet®️ BLI is ideally suited to downstream optimization processes, such as high-affinity binding studies.

00:50 Its real-time, label-free approach to lead optimization provides crucial information on binding specificity and affinity, and allows you to monitor critical quality attributes during biologics development. Octet®️ BLI saves time and offers valuable insights to help characterize potential leads more efficiently.

01:10 Octet®️ biolayer interferometry systems also fit seamlessly into the preclinical development phase. The precise and sensitive nature of this technology makes it suitable for immunogenicity studies, where you can measure exact antibody responses after treatment or vaccination.

01:27 When a drug reaches the process development phase, the Octet®️ BLI can support numerous applications. For example, real-time target concentration measurements can be used to optimize cell line development, by aiding the selection of growth media and conditions.


01:44 Finally, the Octet®️ BLI instrument family can be used in a wide range of quality control applications, offering easy measurement of protein stability, potency and activity.

01:55 Whatever your application or stage in the drug development pipeline, the Octet®️ BLI platform provides a scalable solution for your biomolecular interaction studies. 

02:06 In summary, Octet®️ biolayer interferometry (BLI) simplifies progress in life sciences and bioprocessing, enabling the development of new and better therapies, and more affordable medicines.

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 #BioLayerInterferometry #BLI #Octet


Octet®️ Platform: BLI Applications

Label-Free High-Quality Kinetics and Quantitation in Real-Time - For over fifteen years, the industry-proven Octet®️ BLI platform has pioneered real-time, robust, label-free analysis for determination of affinity, kinetics and analyte quantitation. Learn more: https://www.sartorius.com/en/products/protein-analysis/octet-bli-detection

Making the characterization of drug candidates significantly easier, faster and better! Octet®️ systems deliver greater value over traditional techniques with limited throughput and performance. Discover the fastest and most flexible system on the market with an enviable range of applications. 

00:02 Science is continuously evolving. And so is the Octet®️ Bio-Layer Interferometry system. 

00:10 Bio-Layer Interferometry technology, or BLI, has been providing researchers with a label-free approach to monitoring biomolecular interactions for over 15 years, overcoming the limitations in throughput, performance and cost associated with traditional technologies.

00:27 Accelerate your workflows and get the results you need to progress your research with Sartorius Octet®️. The path to progress just got simpler.

00:38 BLI is an optical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on a biosensor tip, and an internal reference layer.  Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern, which is recorded in real time.

00:59 A proprietary protein coating at the biosensor tip acts as a binding partner, providing reproducible coupling of target molecules while minimizing non-specific binding. The measurement is plate-based, and aqueous buffer is used to generate a baseline, before the sensor dips into a sample containing the analyte. 

01:18 The analyte then binds to the sensor surface, and the binding interaction is followed in real time to reveal the association kinetics. The sensor tip is then dipped into fresh buffer, and the dissociation can be monitored until the regeneration of the biosensor surface. 

01:36 This real-time analysis provides precise and accurate data on binding specificities, analyte concentrations, and rates of association and dissociation.

01:48 A unique characteristic of BLI is that only analyte binding or dissociation can shift the interference pattern and generate a response profile. Unbound molecules, changes in the refractive index or pH, or other matrix effects do not affect results, making this technology well suited to measurements in crude samples. 

02:09 In addition, BLI is easy to use, making assay development and optimization a much faster process, for a shorter time to results. This fluidic-free approach eliminates sample loading or cleaning steps between measurements.

02:23 Finally, BLI is easily scalable. With the sensors arrayed in a standard SLAS-format, you can perform measurements with multiple sensor tips in parallel to perfectly match individual labs’ throughput needs.

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 #BioLayerInterferometry​ #BLI #OctetBLI

Octet®️ Platform: Bio-Layer Interferometry (BLI) Technology Introduction

Danaher Life Sciences

Danaher Life Sciences is your partner for workflow innovation and end-to-end solutions.

Every day, scientists around the world are working to understand the causes of disease, develop new therapies and vaccines and test new drugs. Danaher Life Sciences, a group of businesses at Danaher Corporation, make this leading-edge scientific research possible. Our capabilities extend beyond research to power the creation of biopharmaceuticals, cell and gene therapies and other breakthrough treatments to advance patient health and improve treatment outcomes.

We see a way to save over 14,000 hours of manual data-handling per year in a cell line development department.
We see a way to identify high producers in <15 minutes in cell line development.  
We see a way to cut the per-sample cost of purifying AAV by 250X vs. affinity chromatography.  
We see a way to accelerate the drug development time curve by 50%.

Talk to an Expert at Danaher Life Sciences today about how you can achieve these outcomes in your workflows.  

Danaher Life Sciences | Together, We See a Way to Accelerate the Drug Development Curve by up to 50%

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CCDC

This is a presentation by Marc Short, from University of Leeds, at the Materials Science Meeting 2021.
In this presentation he's talking about virtual screening of ligands for homogeneous catalysis using CSD-CrossMiner.

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