Automation

TotalLab

In this video, I'll show you how you can obtain quantifiable data from lateral flow tests (LFT/LFA) in a 21 CFR Part 11/GxP/EU Annex 11 compliant manner using Phoretix 1D, which allows you to produce a full audit trail for every single lateral flow test you analyse to ensure regulatory compliance.

00:00 - Intro
00:20 - Explanation of TotalLab GxP Compliance Module
00:50 - How to log in to the software
01:40 - Username entry
01:50 - Password entry
02:17 - 21 CFR Sign in
02:23 - Project screen overview
02:32 - Creating a new compliant project
02:46 - Attaching notes to the audit trail of a project
03:10 - Adding files to your project
03:42 - Audit trail explanation
04:20 - Checking out a project for analysis
04:32 - Software integrations with the TotalLab GxP module
04:39 - Opening Phoretix 1D analysis software
04:50 - Analysing lateral flow tests
10:27 - Saving your analysis
10:32 - Audit trail build-up explanation
10:48 - Extended notes within the audit trail
11:40 - Checking a project back in to the secure folder
12:05 - Adding notes to a project check-in
12:23 - Checking the revision number of a project
12:54 - Viewing previous revisions of a project
13:08 - Viewing changes in data between revisions
13:41 - Exporting a 21 CFR compliant project report in .pdf
14:00 - Overview of report
15:20 - Sending a project for sign-off
15:50 - Adding notes for project sign-off to the audit trail
17:22 - Signing off a project
17:36 - Identifying projects awaiting sign-off
17:50 - Audit trail for project awaiting sign-off
18:36- Checking out a read-only copy of a project for inspection
19:20 - Password-protected sign-off
19:35 - Adding notes to project sign-off
21:05 - Project signed-off and closed

Quantifying the bands allows accurate comparison of the reactivity of different antibodies to positive samples and the definition of the limits of detection of those antibodies to positive samples (to determine the minimum testing sample volume).

A previous version of our 1D analysis software, CLIQS, has already been used for this purpose with the research published here: https://www.mdpi.com/2079-6374/11/12/510/htm

Another of our products, CLIQS 1D Pro, allows the building of a database of lateral flow tests that can be searched or allow tests to be compared to the entire database for similarities and differences.

For a free trial of Phoretix 1D to see how well it works with your lateral flow test images, please fill in the form here: https://totallab.com/products/phoretix-1d-free-trial/

For a free trial of CLIQS 1D Pro to see how well it works creating a database of your lateral flow test images, please fill in the form here: https://totallab.com/products/cliqs-1d-pro-free-trial/

For a free trial of our Phoretix 1D GxP software, to see how well it operates within a 21 CFR Part 11/GxP/Annex 11 compliant format, please fill in the form here:
https://totallab.com/products/gxp-module-demo/

#21CFR #GxP #lateralflowtest #LFA #analysis #GxP #database #software #LFT  #1D #imageanalysis #automatic #howto #antibody #testing

How to Analyse Lateral Flow Tests (LFT/LFA) in compliance with 21 CFR Part 11 Using Phoretix 1D

Calibration is an important part of some user's workflows, allowing harmonisation across your labs or even within the same lab using different imaging devices such as gel docs or densitometers to scan your immunoblots or electrophoresis gels. 

Our TotalLab GxP software fully supports calibration of images to convert pixel intensity to optical density or whatever other calibrated metric you wish to use. 

00:00 - Intro
01:35 - Why calibrating images is important
01:55 - How to import your stepwedge
02:45 - How to open the calibration mode
03:08 - False colour heatmap
04:08 - Creating a calibration map
05:20 - Quick sampling stepwedge to define steps
05:55 - Manual sampling points for calibration
06:30 - Standard curve/line of best fit for calibration data
08:40 - Equations for calibration data fitting
11:20 - Saving calibration files
13:20 - Applying calibration file to an image

Calibration is compatible with 1D or 2D images and our Phoretix or SameSpots/SpotMap image analysis products.
 
See our other image analysis-based products here: https://totallab.com/products/ where you can request a free trial of our software.

And if you want to get in touch with us here at TotalLab:

Website: http://www.totallab.com/ 
LinkedIn: https://www.linkedin.com/company/totallab-ltd/

#calibration #imagecalibration #westernblot #electrophoresis #SDS-PAGE #tlc #quantification #immunoblot #software #imageanalysis #totallab1d #imagej #21cfr #FDA #lifescience

How to Calibrate 1D or 2D Gels or Blots Using a Stepwedge in Phoretix 1D

Thermo Fisher Scientific | qPCR

Change the world, or change hers. Discover how Thermo Fisher Scientific can put you in control of assay development and testing in molecular diagnostics. 

Learn more: 
https://www.thermofisher.com/us/en/home/clinical/clinical-genomics/molecular-diagnostics-solutions.html

Contact a QuantStudio systems specialist for purchase support:
https://www.thermofisher.com/us/en/home/global/forms/life-science/quantstudio-dx-quote.html

For In Vitro Diagnostic Use.

Real-Time PCR Solutions for Molecular Diagnostics

Hamilton Company (Robotics)

In this video, Oxford Biomedica, an innovative global gene and cell therapy company with operations in the UK and US, describes the automation of an assay requiring a 10-day cell-based workflow, using an integrated Hamilton Microlab STAR liquid handler and CECULA's Graphical User Interface (GUI).

Download the Case Study here: https://hubs.li/Q01xk4kF0

Automating Cell-Based Assays to Determine Viral Vector Titer – Oxford Biomedica & Hamilton Robotics

CloudLIMS

Watch our webinar to learn how LIMS helps food testing laboratories meet FSMA requirements for food safety & automate workflows with ease. Learn more: https://cloudlims.com/resources/lims-webinar/

Webinar: Learn How a LIMS Enables Food Testing Labs To Meet FSMA Requirements

The webinar highlights how a cloud-based LIMS is a technologically-advanced and cost effective alternative to traditional on-premise LIMS.

KEY TAKEAWAYS:
- Data management challenges for clinical laboratories
- Regulations and guidelines to follow
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Webinar: Data Privacy and Other Clinical Data Management Challenges

CloudLIMS.com and Rajiv Gandhi Cancer Institute and Research Center, India organized a live webinar. The webinar provided valuable information about the challenges and opportunities faced by biorepositories and research labs by focusing on the role of biobanking in facilitating high-quality cancer research that helps enhance health and well-being.

Watch now to understand:
1. Why is Biobanking Imperative for the Progress of Clinical Research?
2. How to Overcome Challenges with Biobank Data Management?
3. Affordable Cloud-based Tools for Efficient Biobanking

LIMS Webinar: Role of Biobanks: Challenges and Opportunities in Cancer Research

Watch the webinar by CloudLIMS which encompasses how a LIMS  solution is crucial for addressing the challenges faced by cannabis testing laboratories. For more information, visit https://cloudlims.com/resources/lims-webinar/

Webinar: Meet Cannabis Testing Standards and Regulatory Requirements with LIMS

LIMS webinar focuses on how a LIMS can help testing laboratories manage data, automate laboratory workflows, and meet regulatory guidelines and quality standards seamlessly. Details on: https://cloudlims.com/resources/lims-webinar/

Automation

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