Analytical Techniques

Aging (Aging-US)

Aging-US published this trending editorial on July 19, 2022 in Volume 14, Issue 14, entitled, "Restoring rhythm to prevent age-related fractures” by researchers Annelies E. Smit, Maaike Schilperoort, and Elizabeth M. Winter from the Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands.

DOI - https://doi.org/10.18632/aging.204192 (PDF Download)

Corresponding author - Elizabeth M. Winter - e.m.winter@lumc.nl

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Keywords - aging, circadian rhythm, fractures, osteoporosis, glucocorticoids, chronotherapy

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Trending With Impact: Restoring Rhythm to Prevent Age-related Fractures

Teach Me in 10

Innovations resulting from science, technology, engineering and mathematics (STEM) fields have positively touched nearly every aspect of human life. Promoting and supporting diversity across areas including gender, age, race, professional sectors and scientific disciplines plays a key role in advancing scientific understanding. Yet, there is no blueprint for bringing diversity to academia and the world of science. Meaningful diversity will not be achieved unless we invest in efforts to make STEM more accessible and inclusive for women and underrepresented populations. 
In this episode of Teach Me in 10, Dr. Jeanita Pritchett, who has a deep-rooted passion for helping others find excitement in STEM and considers herself a "Jane of All Trades", will speak about what diversity means and why DEI policies and practices are an important factor in optimizing the science ecosystem. She will also explain how she seeks to address the question of what everyone can do to help create a more inclusive environment. 
Dr. Pritchett will share steps that need to be taken to effect real, tangible and lasting change in the scientific community, or what Extraordinary Grace calls “The Change Hypothesis”. This hypothesis is the result of more than 1,300 scientists and researchers like yourselves from around the globe opening-up to Grace with their raw and honest experiences when she asked, "Can I be a scientist, and can I still be me?”


Diversity, Equity and Inclusion in STEM With Dr. Jeanita Pritchett

Handheld Raman Spectrometer

Finder Insight Portable Raman Analyzer is a high-performance Raman Spectrometer suitable for portable transportation. It can be applied to on-site rapid inspection and laboratory research. It is used in extensive applications, such as food and drug safety, drug testing, dangerous chemicals testing, pharmaceutical engineering, pharmaceutical process (PAT), etc

Portable Raman Analyzer

In the second installment of the Longevity & Aging Series, Dr. Steve Horvath revisits papers he co-authored that were published by Aging (Aging-US) and featured as a special collection of his research.

Special Collection - https://www.aging-us.com/special-collections-archive/steve-horvath
Author contact - Steve Horvath - shorvath@mednet.ucla.edu

Papers - 

DNA-methylation-based telomere length estimator: comparisons with measurements from flow FISH and qPCR
https://doi.org/10.18632/aging.203126

Epigenetic mutation load is weakly correlated with epigenetic age acceleration
https://doi.org/10.18632/aging.103950

Blood DNA methylation sites predict death risk in a longitudinal study of 12, 300 individuals
https://doi.org/10.18632/aging.103408

Epigenome-wide association study of leukocyte telomere length
https://doi.org/10.18632/aging.102230

DNA methylation-based estimator of telomere length
https://doi.org/10.18632/aging.102173

Optimism is not associated with two indicators of DNA methylation aging
https://doi.org/10.18632/aging.102090

Placental epigenetic clocks: estimating gestational age using placental DNA methylation levels
https://doi.org/10.18632/aging.102049

Rapamycin retards epigenetic ageing of keratinocytes independently of its effects on replicative senescence, proliferation and differentiation
https://doi.org/10.18632/aging.101976

Epigenetic clock analysis of human fibroblasts in vitro: effects of hypoxia, donor age, and expression of hTERT and SV40 largeT
https://doi.org/10.18632/aging.101955

DNA methylation GrimAge strongly predicts lifespan and healthspan
https://doi.org/10.18632/aging.101684

Cell and tissue type independent age-associated DNA methylation changes are not rare but common
https://doi.org/10.18632/aging.101666

A multi-tissue full lifespan epigenetic clock for mice
https://doi.org/10.18632/aging.101590

Epigenetic ageing is distinct from senescence-mediated ageing and is not prevented by telomerase expression
https://doi.org/10.18632/aging.101588

Epigenetic clock for skin and blood cells applied to Hutchinson Gilford Progeria Syndrome and ex vivo studies
https://doi.org/10.18632/aging.101508

An epigenetic biomarker of aging for lifespan and healthspan
https://doi.org/10.18632/aging.101414

Leukocyte telomere length, T cell composition and DNA methylation age
https://doi.org/10.18632/aging.101293

Accelerated epigenetic aging in Werner syndrome
https://doi.org/10.18632/aging.101217

An epigenetic aging clock for dogs and wolves
https://doi.org/10.18632/aging.101211

Longitudinal study of surrogate aging measures during human immunodeficiency virus seroconversion
https://doi.org/10.18632/aging.101184

Epigenetic clock analysis of diet, exercise, education, and lifestyle factors
https://doi.org/10.18632/aging.101168

Specific premature epigenetic aging of cartilage in osteoarthritis
https://doi.org/10.18632/aging.101053

DNA methylation-based measures of biological age: meta-analysis predicting time to death
https://doi.org/10.18632/aging.101020

Huntington's disease accelerates epigenetic aging of human brain and disrupts DNA methylation levels
https://doi.org/10.18632/aging.101005

Epigenetic age of the pre-frontal cortex is associated with neuritic plaques, amyloid load, and Alzheimer’s disease related cognitive functioning
https://doi.org/10.18632/aging.100864

Decreased epigenetic age of PBMCs from Italian semi-supercentenarians and their offspring
https://doi.org/10.18632/aging.100861

Increased epigenetic age and granulocyte counts in the blood of Parkinson's disease patients
https://doi.org/10.18632/aging.100859

DNA methylation age of blood predicts future onset of lung cancer in the women's health initiative
https://doi.org/10.18632/aging.100809

Epigenetic age analysis of children who seem to evade aging
https://doi.org/10.18632/aging.100744

The cerebellum ages slowly according to the epigenetic clock
https://doi.org/10.18632/aging.100742

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ or connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Longevity & Aging Series (EP 2): Dr. Steve Horvath’s Special Collection in Aging

Aging-US published this trending research paper on July 8, 2022 in Volume 14, Issue 14, entitled, "Short-term resveratrol treatment restored the quality of oocytes in aging mice" by researchers from the Department of Obstetrics and Gynecology, International University of Health and Welfare School of Medicine, Chiba 286-8686, Japan; Department of Biodesign, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University Chiyoda-ku, Tokyo 101-0062, Japan; Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine Bunkyo-ku, Tokyo 113-8421, Japan; Aging Stress Response Project Team, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan.

DOI - https://doi.org/10.18632/aging.204157

Corresponding author - Kazuhiro Kawamura - k.kawamura.aq@juntendo.ac.jp

Abstract

The quality of oocytes declines by aging, resulting in their low competences for fertility. Here, resveratrol treatment showed increases in the rates of implantation and live offspring as well as decreases in the abortion rate as short as one week after treatment, although the number of ovulated oocytes and the rates of fertilization and blastocyst formation were not changed following resveratrol treatment. Resveratrol treatment did not cause abnormalities mouse estrous cycles and body weights. No abnormality was detected in both fetuses and placentas after 22 weeks of resveratrol treatment and the fetuses had normal fertility. Positive correlations were found between serum resveratrol levels and pregnancy and live offspring rates as well as ovarian expression levels of Sirt1, Sirt3, Sirt4, Sirt5, and Sirt7. The mitochondrial membrane potential and ATP content but not copy number of mitochondrial DNA in oocytes was increased in aging mice with resveratrol treatment. In conclusion, we demonstrated the restoration of oocyte quality in aging mice in addition to the prevention of their quality decline during aging by restoring mitochondrial functions by resveratrol treatment without adverse effects in the animals and their offspring.

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Keywords - aging, resveratrol, age-associated infertility, oocyte quality, Sirtuin, mitochondria

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Trending With Impact: Resveratrol Treatment Restored Quality of Oocytes in Aging Mice

Technology Networks

Speaking at Advances in Food and Beverage Analysis 2021, Andrea Hochegger, from Graz University of Technology, presented her talk on the analysis of mineral oil hydrocarbons in food.

Mineral Oil Hydrocarbons in Food - An Analytical Challenge

Organomation

Solvent Compatibility Table: https://fs.hubspotusercontent00.net/hubfs/6549100/Updated%20Solvent%20Table.pdf

Related Article: https://blog.organomation.com/blog/organomation-publishes-solvent-table-for-laboratory-evaporators

Contact Us: https://www.organomation.com/contact

                    Solvent Compatibility for Your Nitrogen Evaporator

Organomation understands that each customer application is unique. That is why our nitrogen evaporators come in a variety of customized  models to accommodate your lab’s specific needs.

There are some important factors to think about before purchasing a nitrogen evaporator. If you will be working with… 
• Corrosive or flammable solvents
• Sensitive samples
• Or solvents with high or low boiling points
…then a customized instrument model may be required. Organomation has four models available, and this video will explain when each is recommended. 

One option we offer is our Teflon coating, which is a corrosion-resistant coating that helps prevent corrosive solvents from damaging the instrument. 

This coating option is recommended when using highly acidic, basic, or otherwise corrosive solvents. Some of the most popular ones being Hydrochloric Acid, Ammonium Hydroxide, Chloroform, and pee-ruh-deen (Pyridine).

For labs working with highly flammable solvents, our team suggests evaporators with a positive pressure purge device, also called a Z-Purge.

The Z-Purge is a safety device that is meant to keep flammable solvent vapor away from the instrument’s heating elements. It maintains a small flow of nitrogen into the bath pan. This creates a slight positive pressure gradient inside the bath case, preventing highly flammable vapors from igniting. 

The device is recommended for any application using highly flammable solvents such as Carbon Disulfide, Diethyl Ether (dai-eh-thuhl ee-thr), Hexane, and Pentane.

In addition to water baths, we offer dry bath models. A dry bath N-EVAP will have aluminum or glass beads as the heating medium, instead of the standard water bath. They are highly recommended when working with water sensitive samples.

The dry bath medium also allows for a higher temperature range than water baths, so this option is recommended when working with solvents that have high boiling points above 90 °C.

For labs using solvents with low boiling points, or heat sensitive samples, unheated instruments are an option. These instruments initiate the evaporation process by nitrogen blowdown only.
They are also a good option for evaporating very small samples that can be effectively dried with only the use of nitrogen blowdown, and not the additional heating element. 

These instrument options are designed to help keep both you and the instrument safe during the evaporation process. They will allow for efficient sample preparation for most applications. 
So how do you know which model is the best choice for your application?

Organomation has published a solvent compatibility table which provides option code recommendations for the most commonly used solvents. A link to this table will be located in the description box below.

If you will be using a solvent that is not listed on the table, or if you have questions on any instrument model, please reach out to sales@organomation.com or 978-838-7300 to speak to one of our sales technicians.

Solvent Compatibility for Your Nitrogen Evaporator || Organomation

Contact Us: https://www.organomation.com/contact

Preventing Sample Contamination in an Evaporation System

Sample contamination is a risk that virtually all labs have to be mindful of. 
Common sources of contamination include compounds in the atmosphere or sample residue left behind on improperly cleaned equipment.
Many of Organomation’s products use nitrogen blowdown as a common evaporation technique. Here are some tips to prevent sample contamination in any nitrogen evaporator.
Make sure that all sample vials are properly cleaned before coming in contact with a sample. They should also be properly stored in a sanitized area between uses.
Similarly, make sure that all gas delivery needles are clear of any residues. It’s recommended to clean and examine the needles between each use.
Organomation also offers pipet adapters, which allow glass pipets to be used in place of stainless steel needles. Since glass pipets are disposable, they are recommended for applications where contamination is a pressing concern.
Use caution when placing sample tubes in the evaporator. Before placing the samples in the unit, raise the needles as high as possible. This will allow tubes to be placed in the holder without tipping.
When lowering the needles back into place, avoid contact with the solvent.

Ensure that your gas source is clean and dry. If any inert gas is being used, it should be high or ultra-high purity depending on the application.

When using atmospheric air from a compressor, a dryer and micro filter may be needed as well.”

It’s also important to avoid excessive sample turbulence due to high gas flow. Solvent splashing may result in sample loss, cross contamination, or needle contamination.

We recommend setting the gas flow rate at a low setting, and slowly increasing it until a dimple is visible on the sample’s surface.

Try to avoid a bath temperature that is too high, which can create condensation on the evaporator. The condensed solvent may drip into the sample or leave residue on the needles. The recommended bath temperature is 2-3°C below the solvent’s boiling point.

Once evaporation is complete, use caution when removing the samples from the unit. Raise the needles as high as possible so that they don’t come in contact with the sample.

Make sure the gas flow is turned off during this step, to avoid any accidental splashing.

Although sample contamination will always be a concern for labs, having strict procedures and properly trained personnel will help reduce the risks. Maintaining these preventative measures will ultimately save the laboratory valuable time and money.

If you have any questions or concerns, please reach out to sales@organomation.com or call 978-838-7300 to speak to one of Organomation’s sales technicians.

Preventing Sample Contamination in an Evaporation System || Organomation

Website: https://www.organomation.com/

Contact Page: https://www.organomation.com/contact

Video Citations:
Khmelevsky, Ruslan, director. Video Of Metal Hook Near Fire. Pexels, https://www.pexels.com/video/video-of-metal-hook-near-fire-4652136/. Accessed 18 July 2022. 
Podrez, Artem, director. Test Tubes with Chemicals. Pexels, https://www.pexels.com/video/test-tubes-with-chemicals-8534430/. Accessed 18 July 2022. 

                                      How to Safely Evaporate Explosive Solvents
Since 1959, Organomation has been a leading provider of sample preparation solutions and equipment. We strive to create long-lasting equipment with safety and reliability as the top priorities.

Many users of Organomation equipment work with highly flammable solvents such as Hexane and Diethyl Ether. That’s why we created a positive pressure purge device as an optional safety item to each of our evaporators and extractors.

This positive pressure purge device, also called a Z-Purge, allows users to safely concentrate samples containing these highly flammable solvents, without the risk of explosion.

The Z-Purge uses both pressurization and purging to protect against an explosion. Pressurization is an affordable yet effective alternative to intrinsically safe or explosion proof equipment.

This device works by maintaining a small flow of inert gas in the heating unit, creating a positive pressure gradient within the enclosure. This prevents solvent vapor from entering the heating unit and igniting. As an additional protection measure, the concentrator is continuously purged with inert gas throughout the entire evaporation process, in order to flush out any stray solvent vapor.

Each Z-Purge device comes with a pressure gauge and control valve to monitor and maintain pressure within the heating unit.

The purge system is not tied to instrument operation, so the evaporator can be operated without a Z-Purge in low-risk environments.

The Z-Purge can be added to all Organomation instruments, including our full line of nitrogen blowdown evaporators as well as our large-scale solvent evaporators and extractors.

For nitrogen blowdown evaporators, the purge line can either be connected to the main nitrogen blowdown line, or to a separate clean air line to conserve nitrogen gas.

If you have any questions on the Z-Purge device, or if you’re not sure whether your application requires one, please reach out to sales@organomation.com or call 978-838-7300 to speak to one of Organomation’s sales technicians.

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