Analytical Techniques

Aging (Aging-US)

Aging-US published this research paper on February 27, 2023 in Volume 15, Issue 5, entitled, "Age-related methylation changes in the human sperm epigenome" by researchers from the Institute of Human Genetics, Julius Maximilians University, Würzburg, Germany; Department of Bioinformatics, Julius Maximilians University, Würzburg, Germany; Platform Degenerative Diseases, German Primate Center, Leibniz Institute for Primate Research, Göttingen and German Center for Cardiovascular Research, Partner Site Göttingen, Göttingen, Germany; Fertility Center, Wiesbaden, Germany.

DOI - https://doi.org/10.18632/aging.204546

Corresponding author - Thomas Haaf - thomas.haaf@uni-wuerzburg.de

Abstract

Advanced paternal age is associated with increased risks for reproductive and offspring medical problems. Accumulating evidence suggests age-related changes in the sperm epigenome as one underlying mechanism. Using reduced representation bisulfite sequencing on 73 sperm samples of males attending a fertility center, we identified 1,162 (74%) regions which were significantly (FDR-adjusted) hypomethylated and 403 regions (26%) being hypermethylated with age. There were no significant correlations with paternal BMI, semen quality, or ART outcome. The majority (1,152 of 1,565; 74%) of age-related differentially methylated regions (ageDMRs) were located within genic regions, including 1,002 genes with symbols. Hypomethylated ageDMRs were closer to transcription start sites than hypermethylated DMRs, half of which reside in gene-distal regions. In this and conceptually related genome-wide studies, so far 2,355 genes have been reported with significant sperm ageDMRs, however most (90%) of them in only one study. The 241 genes which have been replicated at least once showed significant functional enrichments in 41 biological processes associated with development and the nervous system and in 10 cellular components associated with synapses and neurons. This supports the hypothesis that paternal age effects on the sperm methylome affect offspring behaviour and neurodevelopment. It is interesting to note that sperm ageDMRs were not randomly distributed throughout the human genome; chromosome 19 showed a highly significant twofold enrichment with sperm ageDMRs. Although the high gene density and CpG content have been conserved, the orthologous marmoset chromosome 22 did not appear to exhibit an increased regulatory potential by age-related DNA methylation changes.

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Keywords - aging, ART outcome, DNA methylation, male germ cells, paternal age effect, human sperm epigenome

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/@AgingJournal
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Age-Related Methylation Changes in the Human Sperm Epigenome

Lumex Instruments 

InfraLUM FT-12 NIR analyzer performs express analysis of various agricultural and food products, allowing you to simultaneously determine main parameters and components with the highest possible accuracy. The analysis is non-destructive and usually does not require any sample preparation.

With regular use, this instrument can significantly reduce analysis cost compared to common wet-chemical methods. The FT spectroscopy technique ensures that InfraLUM FT-12 performs
a very precise and reproducible analysis. 

Lumex Instruments offers another InfraLUM FT-12 WholeGrain model with the automatic cell filling for the analysis of whole grains.


Express NIR spectrometer InfraLUM FT-12

Life Solved

How can psychology be used to make those decisions work for you… and the planet?

This is Life Solved, the research podcast from the University of Portsmouth, where we explore how breakthroughs here are changing our world today and in the future.

This time – how can we address climate change through public perception? Dr Chris Jones is a Reader in Applied Psychology at the University Of Portsmouth and  Director of the Quality Of Life, Health & Wellbeing Group at the University of Portsmouth. He believes we can all be positively influenced to live more sustainable lifestyles.

To listen to the full episode search 'Life Solved' wherever you listen to podcasts, or click here: https://podfollow.com/uoplifesolved/view 

You can find out more about this work and other research at the University of Portsmouth website: 
https://www.port.ac.uk/research/research-projects


The Power of Public Perception in the Fight Against Climate Change | Life Solved

Creative Enzymes

Creative Enzymes is a worldwide leading company in diagnostic enzymes manufacturing and supply. We are committed to providing our customers with diverse enzyme products and custom enzyme-related services for medical and research diagnosis. Relying on our professional team and state-of-art technologies, we have gained solid reputation for having top of the line products and services.

Our product lines cover a wide range of application areas, including but not limited to blood lipids, liver function, kidney and pancreas function, diabetes, POCT bio-sensor and clinical diagnosis. Every product is presented with detailed description, source, form, activity and other biochemical properties. Our high-quality products always ensure the rapid, reliable and reproducible experiments.  https://diagnostic-enzymes.creative-enzymes.com/

Diagnostic Enzymes

Teach Me in 10

March 8 marks [url][link]https://www.internationalwomensday.com/[/link][name]International Women’s Day 2023[/name][/url], a global holiday that celebrates women’s rights movements and brings focus to issues such as gender equality, reproductive rights and violence against women.

To celebrate International Women’s Day, Technology Networks is delighted to showcase the work of [url][link]https://pharmacy.auburn.edu/directory/angela-calderon.php[/link][name]Dr. Angela Calderón[/name][/url], associate professor in drug discovery and development at Auburn University in the United States.

At Auburn University, Dr. Calderón’s research interests lie in the application of mass spectrometry to study enzyme kinetics and ligand–enzyme interactions, particularly in the context of natural products drug discovery. Beyond the contributions her laboratory has made in botanical drug development, Dr. Calderón is also an advocate for women in science – recruiting only female scientists to her laboratory. She is also a passionate mentor for women in science, receiving the [url][link]https://ocm.auburn.edu/newsroom/campus_notices/2023/03/030847-calderon.php[/link][name]2023 Female Scientist Pharmacist Research Excellence Award[/name][/url] and the [url][link]https://pharmacy.auburn.edu/news/2020-21/120120-calderon-award.php[/link][name]2020 Provost Award for Faculty Excellence in Undergraduate Research Mentoring[/name][/url].

Join us for this Teach Me in 10 video to learn more about Dr. Calderón’s research and how she is championing efforts to support women in science and engineering roles.

Celebrating Female Scientists on International Women’s Day

Analytical Cannabis 

This exclusive all-female panel discussion took place in February 2023 to honor International Women and Girls in Science Day.

We were delighted to host expert female cannabis scientists for this panel who discussed challenges and opportunities in cannabis testing and regulations from their unique perspective as women actively engaged in these lines of work. 

This panel discussion explored:
- Analytical challenges alongside regulatory limits
- Testing to compliance and realistic regulations
- Challenges and updates of the cannabis industry in Oklahoma and Florida

Women in Cannabis Science

Oncotarget

Oncotarget published this review in Volume 14, entitled, “What is hemoglobin, albumin, lymphocyte, platelet (HALP) score? A comprehensive literature review of HALP’s prognostic ability in different cancer types" by researchers from the Department of Urology, George Washington University School of Medicine, Washington, DC; Department of Surgery, George Washington University School of Medicine, Washington, DC.

DOI - https://doi.org/10.18632/oncotarget.28367

Correspondence to - Christian Mark Farag - cmfarag@gwu.edu

Abstract

Since its inception, the Hemoglobin, Albumin, Lymphocyte, Platelet Score (HALP) has gained attention as a new prognostic biomarker to predict several clinical outcomes in a multitude of cancers. In our review, we searched PubMed for articles between the first paper on HALP in 2015 through September 2022, yielding 32 studies in total that evaluated HALP's association with various cancers, including Gastric, Colorectal, Bladder, Prostate, Kidney, Esophageal, Pharyngeal, Lung, Breast, and Cervical cancers, among others. This review highlights the collective association HALP has with demographic factors such as age and sex in addition to TNM staging, grade, and tumor size. Furthermore, this review summarizes HALP's prognostic ability to predict overall survival, progression-free survival, recurrence-free survival, among other outcomes. In some studies, HALP has also been able to predict response to immunotherapy and chemotherapy.

This review article also aims to serve as a comprehensive and encyclopedic report on the literature that has evaluated HALP as a biomarker in various cancers, highlighting the heterogeneity surrounding HALP's utilization. Because HALP requires only a complete blood count and albumin - already routinely collected for cancer patients - HALP shows potential as a cost-effective biomarker to aid clinicians in improving outcomes for immuno-nutritionally deficient patients.

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Keywords - HALP score, biomarker, prognosis, cancer, survival

About Oncotarget

Oncotarget is a primarily oncology-focused, peer-reviewed, open access journal. Papers are published continuously within yearly volumes in their final and complete form, and then quickly released to Pubmed. On September 15, 2022, Oncotarget was accepted again for indexing by MEDLINE. Oncotarget is now indexed by Medline/PubMed and PMC/PubMed.

To learn more about Oncotarget, please visit https://www.oncotarget.com and connect with us:

SoundCloud - https://soundcloud.com/oncotarget
Facebook - https://www.facebook.com/Oncotarget/
Twitter - https://twitter.com/oncotarget
Instagram - https://www.instagram.com/oncotargetjrnl/
YouTube - https://www.youtube.com/@OncotargetJournal
LinkedIn - https://www.linkedin.com/company/oncotarget
Pinterest - https://www.pinterest.com/oncotarget/
Reddit - https://www.reddit.com/user/Oncotarget/

Media Contact
MEDIA@IMPACTJOURNALS.COM
18009220957

What is Hemoglobin, Albumin, Lymphocyte, Platelet (HALP) Score?

Speaking at an Analytical Cannabis webinar in November 2022, Sabrina Didado, Founder/ and Biochemist of Cosmic Harvest, LLC , presented insights into the latest real-world solutions for cannabis oil purification and contaminant remediation with a focus on production-scale solutions.

Talk abstract: With the influx of varying quality cannabis and hemp flower on the market today, proper processing is one of the most critical components of obtaining accurate and precise products for consumers to count on. Even for vertically integrated companies, the variation and unpredictability from plant to plant and harvest to harvest can greatly change the rigid and precision of the processing system. 
This webinar will provide insights into the latest real-world solutions for cannabis oil purification and contaminant remediation with a focus on production-scale solutions. Sabrina will briefly explain the different processes of cannabis extraction and the various steps involved to obtain a desired cannabinoid potency. Terpene isolation will also be mentioned briefly. Then, quality assurance will be discussed in detail and how important this step is to the end consumer. Different options for contamination remediation will also be mentioned, as well as preventative measures. 


Cannabis Oil Purification and Contaminant Remediation 

TotalLab

In this video, I'll show you how you can obtain quantifiable data from lateral flow tests (LFT/LFA) in a 21 CFR Part 11/GxP/EU Annex 11 compliant manner using Phoretix 1D, which allows you to produce a full audit trail for every single lateral flow test you analyse to ensure regulatory compliance.

00:00 - Intro
00:20 - Explanation of TotalLab GxP Compliance Module
00:50 - How to log in to the software
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02:17 - 21 CFR Sign in
02:23 - Project screen overview
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04:32 - Software integrations with the TotalLab GxP module
04:39 - Opening Phoretix 1D analysis software
04:50 - Analysing lateral flow tests
10:27 - Saving your analysis
10:32 - Audit trail build-up explanation
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11:40 - Checking a project back in to the secure folder
12:05 - Adding notes to a project check-in
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18:36- Checking out a read-only copy of a project for inspection
19:20 - Password-protected sign-off
19:35 - Adding notes to project sign-off
21:05 - Project signed-off and closed

Quantifying the bands allows accurate comparison of the reactivity of different antibodies to positive samples and the definition of the limits of detection of those antibodies to positive samples (to determine the minimum testing sample volume).

A previous version of our 1D analysis software, CLIQS, has already been used for this purpose with the research published here: https://www.mdpi.com/2079-6374/11/12/510/htm

Another of our products, CLIQS 1D Pro, allows the building of a database of lateral flow tests that can be searched or allow tests to be compared to the entire database for similarities and differences.

For a free trial of Phoretix 1D to see how well it works with your lateral flow test images, please fill in the form here: https://totallab.com/products/phoretix-1d-free-trial/

For a free trial of CLIQS 1D Pro to see how well it works creating a database of your lateral flow test images, please fill in the form here: https://totallab.com/products/cliqs-1d-pro-free-trial/

For a free trial of our Phoretix 1D GxP software, to see how well it operates within a 21 CFR Part 11/GxP/Annex 11 compliant format, please fill in the form here:
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#21CFR #GxP #lateralflowtest #LFA #analysis #GxP #database #software #LFT  #1D #imageanalysis #automatic #howto #antibody #testing

Analytical Techniques

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