Cell Culture

CD Genomics

RNA-Seq is the premier tool for mapping and quantifying transcriptomes by utilizing next-generation sequencing (NGS) technology. The transcriptome refers to the complete set of transcripts in a cell, which provides information on the transcript level for a specific developmental stage or physiological condition. Understanding the transcriptome is necessary for interpreting the functional elements of the genome and understanding development and disease. The key purpose of transcriptomics includes cataloging all species of transcripts; determining the transcriptional structure of genes; and quantifying the expression levels of each transcript under different conditions.

The Development and Workflow of RNA-seq, Learn more at https://www.cd-genomics.com/resourse-Bioinformatics-Workflow-of-RNA-Seq.html

Principle and Workflow of RNA Sequencing

CD Genomics has extensive experience in providing support for the selection and design of microsatellite markers for a wide range of plant and animal species. Besides microsatellite genotyping, we also provide genotyping by sequencing (GBS) method, which saves time and resources.  https://www.cd-genomics.com/Microsatellite-Genotyping-Service.html

Microsatellite Genotyping Service  at CD Genomics

Teach Me in 10

Cell culture media is one of the most important components of the in vitro tissue environment. It provides the nutrients, minerals and growth factors necessary to sustain cells outside the body. 

This Teach Me in 10 explores the different media options available for plating mammalian cells. It covers preparation techniques, storage options, and how to choose the right media for your experiment.

Plating Cells: Preparation and Media Options With Ann Rossi Bilodeau

There is an undeniable art to bench science. From simply pouring a polyacrylamide gel to the more gruelling task of single-cell isolation. Scientists spend years mastering these techniques, but art has its limits. For example, a biopharmaceutical company developing therapeutic monoclonal antibodies needs a fast and reliable way to isolate thousands of B- cell clones or hybridomas. Automated, single-cell isolation systems with precision robotics like CellCelector address this critical need, by providing one-day workflows, with significant benefits compared to fluidics-based alternatives. 

In this episode we speak with Andris Abramenkovs, product manager of CellCelector technology at Sartorius, about how CellCelector transforms single-cell screening and cloning workflows, the unique nanowell arrays, and some of the applications with this technology.

Single-Cell Isolation: When Art is Not Enough

Aging (Aging-US)

Aging-US published this trending research paper in Volume 14, Issue 21, entitled, "ESR1 dysfunction triggers neuroinflammation as a critical upstream causative factor of the Alzheimer’s disease process" by researchers from NatPro Center, School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Ireland; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China; Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, China; School of Medicine, Trinity College Dublin, Ireland.

DOI - https://doi.org/10.18632/aging.204359

Corresponding authors - Junying Liu - juliu@tcd.ie 

Abstract

Alzheimer's disease (AD) accounts for approximately 60% of dementia cases worldwide. Advanced age is the most significant risk factor for AD and approximately two-thirds of cases relate to women. While the previous meta-analysis suggests that estrogen receptor (ESR) genetic polymorphisms are closely associated with dementia, the implications of this observation on a molecular level are not entirely understood. Our study explores this intricate molecular puzzle through the use of a variety of bioinformatics tools. Initially, we attempted to elucidate mechanisms underlying breast cancer development by identifying the high-throughput dataset of ESR1-knockdown breast cancer tissue samples. Surprisingly, KEGG pathway enrichment showed that the most frequently occurring proteins were related to axonal guidance and inflammation-related gene markers. These observations were supported by an external high throughput dataset of AD inflammatory samples in vivo. Our results suggest that ESR1 is modulated by apolipoprotein E (APOE) through CEBPB/ATF4, mir-155-5p, or mir-1-3p. Moreover, sea hare-hydrolysates (SHH), as one of the axonal guidance molecules, could regulate the STAT3/PRDM1/CEBPB pathway and consequently induce cell death through pyroptosis signaling pathways, trigger the secretion of IL1β, leading to neuroinflammation and worsening AD pathogenesis. Molecular docking verification demonstrated that the predicted natural products scoulerine and genistein displayed strong binding affinities for BACE1 and ESR1, respectively. This strategy can be used to design novel, personalized therapeutic approaches to treatment and a first-in-class clinical lead for the personalised treatment of AD.

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Keywords - ESR1, Alzheimer’s disease, CEBPB/ATF4, APOE, pyroptosis

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

ESR1: A Critical Factor in the Alzheimer’s Disease Process

Oncotarget

Oncotarget published this research paper in Volume 13, entitled, "The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells" by researchers from Center for Myeloma Research, Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; Department of Immunology and Transfusion Medicine, St. Olav's University Hospital, Trondheim, Norway; Laboratory Clinic, St. Olav's University Hospital, Trondheim, Norway; Department of Hematology, St. Olav's University Hospital, Trondheim, Norway.

DOI - https://doi.org/10.18632/oncotarget.28300

Correspondence to - Ida Steiro - ida.j.steiro@ntnu.no

Abstract

Background: Multiple myeloma (MM) is an incurable malignancy of plasma cells. The serine protease matriptase is frequently dysregulated in human carcinomas, which facilitates tumor progression and metastatic dissemination. The importance of matriptase in hematological malignancies is yet to be clarified. In this study, we aimed to characterize the role of matriptase in MM.

Materials and Methods: mRNA expression of matriptase and its inhibitors hepatocyte growth factor activator inhibitor (HAI)-1 and HAI-2 was studied in primary MM cells from patient samples and human myeloma cell lines (HMCLs). We further investigated the effect of matriptase on migration and proliferation of myeloma cells in vitro. By use of the CoMMpass database, we assessed the clinical relevance of matriptase in MM patients.

Results: Matriptase was expressed in 96% of patient samples and all HMCLs tested. Overexpression of matriptase in vitro reduced proliferation, and significantly decreased cytokine-induced migration. Conversely, matriptase knockdown significantly enhanced migration. Mechanistically, overexpression of matriptase inhibited activation of Src kinase.

Conclusions: Our findings may suggest a novel role of matriptase as a tumor suppressor in MM pathogenesis.

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Keywords - matriptase, multiple myeloma, tumor suppressor, migration, Src

About Oncotarget

Oncotarget is a primarily oncology-focused, peer-reviewed, open access journal. Papers are published continuously within yearly volumes in their final and complete form, and then quickly released to Pubmed. On September 15, 2022, Oncotarget was accepted again for indexing by MEDLINE. Oncotarget is now indexed by Medline/PubMed and PMC/PubMed.

To learn more about Oncotarget, please visit https://www.oncotarget.com and connect with us:

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Reddit - https://www.reddit.com/user/Oncotarget/

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18009220957

Paper Spotlight: Matriptase Inhibits Migration and Proliferation in Multiple Myeloma Cells

Aging-US published this research paper as the cover of Volume 14, Issue 20, entitled, "Downregulation of senescence-associated secretory phenotype by knockdown of secreted frizzled-related protein 4 contributes to the prevention of skin aging" by researchers from the Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Shinjuku, Tokyo, Japan.

DOI - https://doi.org/10.18632/aging.204273

Corresponding authors - Kento Takaya - kento-takaya312@keio.jp

Abstract

There is growing evidence that the appearance and texture of the skin that is altered during the aging process are considerably enhanced by the accumulation of senescent dermal fibroblasts. These senescent cells magnify aging via an inflammatory, histolytic, and senescence-associated secretory phenotype (SASP). Secreted frizzled-related protein 4 (SFRP4) was previously determined to be expressed in dermal fibroblasts of aging skin, and its increased expression has been shown to promote cellular senescence. However, its role in the SASP remains unknown. We found that SFRP4 was significantly expressed in p16ink4a-positive human skin fibroblasts and that treatment with recombinant SFRP4 promoted SASP and senescence, whereas siRNA knockdown of SFRP4 suppressed SASP. Furthermore, we found that knockdown of SFRP4 in mouse skin ameliorates age-related reduction of subcutaneous adipose tissue, panniculus carnosus muscle layer, and thinning and dispersion of collagen fibers. These findings suggest a potential candidate for the development of new skin rejuvenation therapies that suppress SASP.

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Press release - https://aging-us.com/news_room/downregulation-of-senescence-associated-secretory-phenotype-by-knockdown-of-secreted-frizzled-related-protein-4-contributes-to-prevention-of-skin-aging
Keywords - aging, skin, fibroblast, SASP, SFRP4

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Downregulation of SASP by SFRP4 Knockdown Helps Prevent Skin Aging

In advanced therapy medicinal products (ATMP) manufacturing, the quality and safety of the product need to be ensured from the first stages of development. 

In this episode, we are joined by Kris Wronski, Cell Culture Applications Scientist from Thermo Fisher Scientific. He will discuss how to design an effective contamination prevention strategy that will minimize risks to cell therapy products and allow you to implement appropriate control measures.

By watching this episode you will learn:
•	Why contamination prevention strategy is so important
•	About the types and sources of particulates in the cell therapy production environment and their impact on product quality and safety 
•	The role of laboratory equipment in contamination prevention and what to look for when sourcing suitable equipment

Contamination Prevention in Cell Therapy Environments With Kris Wronski

Dr. Takushi Namba, Associate Professor at Kochi University in Japan, discusses a research paper he co-authored that was published by Aging (Aging-US) in Volume 14, Issue 19, entitled, “Lotus germ extract rejuvenates aging fibroblasts via restoration of disrupted proteostasis by the induction of autophagy.”

DOI - https://doi.org/10.18632/aging.204303

Corresponding author - Takushi Namba - t-namba@kochi-u.ac.jp

Video transcript - https://aging-us.net/2022/10/27/behind-the-study-lotus-germ-extract-rejuvenates-aging-fibroblasts/

Abstract

Cell aging attenuates cellular functions, resulting in time-dependent disruption of cellular homeostasis, which maintains the functions of proteins and organelles. Mitochondria are important organelles responsible for cellular energy production and various metabolic processes, and their dysfunction is strongly related to the progression of cellular aging. Here we demonstrate that disruption of proteostasis attenuates mitochondrial function before the induction of DNA damage signaling by proliferative and replicative cellular aging. We found that lotus (Nelumbo nucifera Gaertn.) germ extract clears abnormal proteins and agglutinates via autophagy-mediated restoration of mitochondrial function and cellular aging phenotypes. Pharmacological analyses revealed that DAPK1 expression was suppressed in aging cells, and lotus germ extract upregulated DAPK1 expression by stimulating the acetylation of histones and then induced autophagy by activating the DAPK1-Beclin1 signaling pathway. Furthermore, treatment of aging fibroblasts with lotus germ extract stimulated collagen production and increased contractile ability in three-dimensional cell culture. Thus, time-dependent accumulation of abnormal proteins and agglutinates suppressed mitochondrial function in cells in the early stage of aging, and reactivation of mitochondrial function by restoring proteostasis rejuvenated aging cells. Lotus germ extract rejuvenates aging fibroblasts via the DAPK1-Beclin1 pathway-induced autophagy to clear abnormal proteins and agglutinates.

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Keywords - aging, mitochondria, autophagy, proteostasis

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at https://www.Aging-US.com​​ and connect with us:

SoundCloud - https://soundcloud.com/Aging-Us
Facebook - https://www.facebook.com/AgingUS/
Twitter - https://twitter.com/AgingJrnl
Instagram - https://www.instagram.com/agingjrnl/
YouTube - https://www.youtube.com/agingus​
LinkedIn - https://www.linkedin.com/company/aging/
Pinterest - https://www.pinterest.com/AgingUS/

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Cell Culture

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