Cell Culture

Lonza

Watch this video to learn how to successfully thaw and plate primary hepatocytes from the experts at Lonza. Not only will you understand the basics of handling your cryopreserved primary hepatocytes, you will also glean tips and tricks to truly maximize your final cell count.
Read the Thawing and Plating Protocol: 
https://bioscience.lonza.com/lonza_bs/US/en/download/product/asset/29887

Learn how Lonza can support your liver research applications: 
https://bioscience.lonza.com/liver-studies-for-adme-tox-studies

Cryopreserved primary hepatocytes products from Lonza: 
https://bioscience.lonza.com/Catalogue/Products/ADME-and-Toxicology/c/5?q=%3A%3A%3A%3Alonza-sort%3A%3A%3A%3Alon_cl_PrimaryCellType%3A%3A%3A%3AHepatocytes


#ADME #Toxicology #Drugmetabolism #pharmacokinetic #DrugDiscovery #DrugDrugInteraction #hepatocytes #primarycells #Lonza

How to Thaw and Plate Primary Hepatocytes | Video Protocol

Fermex

Fermex BioFMA Autoclavable Fermenter & Bioreactor
Model : BioFMA
Capacity : 1L to 10L
Type : Autoclavable (Bench-top)
To know more about "Fermex BioFMA Autoclavable Fermenter & Bioreactor"
Click here: https://bit.ly/3oJR0Cn & https://bit.ly/3otQqrY

Please contact us for more information:
Email:  info@fermex.in & sales@fermex.in
Phone: +91-172-2972149, +91-81818-18529, +91-9592296100,
Website:  https://www.fermex.in

Fermex BioFMA Autoclavable Fermenter & Bioreactor | Benchtop | Lab scale

CD Genomics

16S/18S/ITS Amplicon Sequencing has now been a well-established method for microbial identification and phylogeny studies of samples from complicated microbiomes or environments. In addition to next-generation sequencing platforms, CD Genomics also provides full-length 16S/18S/ITS amplicon sequencing by using PacBio SMRT sequencing technology.	 https://www.cd-genomics.com/16S-18S-ITS-Amplicon-Sequencing.html

Bioinformatics analysis of 16S rRNA sequencing data: Amplicon Sequencing

Watch our expert, Maureen Bunger, PhD, explain how our patented manufacturing process ensures that the DonorPlex™ Hepatocytes exhibit improved phenotype reproducibility. Additionally, we have a proprietary algorithm that predicts yield, viability and functional phenotype prior to manufacture which ensures individual customer specifications are met.

Learn more about DonorPlex™ Pooled Donor Human Hepatocytes:
https://bioscience.lonza.com/pooled-human-donor-hepatocytes

Learn how Lonza can support your liver research applications:
https://bioscience.lonza.com/liver-studies-for-adme-tox-studies

DonorPlex™ Pooled Donor Human Hepatocytes products from Lonza:
https://bioscience.lonza.com/search/?text=donorplex


#ADME #Toxicology #Drugmetabolism #pharmacokinetic #DrugDiscovery #hepatocytes #primarycells #FDA #Lonza #DrugDrugInteraction #DDI

DonorPlex™ Pooled Donor Human Hepatocytes Enable the Next Level of Reliability | Lonza

Advance your in vitro drug interaction studies to the next level! Watch this video to learn how our Interaction Qualified primary hepatocytes can increase your confidence in your DDI and metabolism studies.

Learn more about Interaction Qualified Primary Hepatocytes: https://bioscience.lonza.com/lonza_bs/US/en/interaction-qualified-hepatocytes


Learn how Lonza can support your liver research applications: https://bioscience.lonza.com/liver-studies-for-adme-tox-studies

Cryopreserved primary hepatocytes products from Lonza: https://bioscience.lonza.com/Catalogue/Products/ADME-and-Toxicology/c/5?q=%3A%3A%3A%3Alonza-sort%3A%3A%3A%3Alon_cl_PrimaryCellType%3A%3A%3A%3AHepatocytes
#ADME #Toxicology #Drugmetabolism #pharmacokinetic #DrugDiscovery #hepatocytes #primarycells #FDA #Lonza #DrugDrugInteraction #DDI

Interaction Qualified Primary Hepatocytes | Lonza Product Overview

Aging

Aging-US published this trending research paper on August 10, 2021, entitled, “Senescence-associated hyper-activation to inflammatory stimuli in vitro” by researchers from the College of Medicine, University of Florida, Gainesville, FL, and the College of Pharmacy, University of Florida, Gainesville, FL.

Abstract
Aging is associated with an increased susceptibility to adverse inflammatory conditions such as sepsis and cytokine storm. We hypothesized that senescent cells (SnCs) play a central role in this age-associated pathology in part due to their expression of the senescence-associated secretory phenotype (SASP), which may prime SnCs to inflammatory stimulation. To test this hypothesis, we examined the expression of various inflammatory cytokines and chemokines at the levels of gene transcription and protein production in various SnCs in vitro in response to lipopolysaccharide (LPS), interleukin-1β (IL1β), and tumor necrosis factor α (TNFα) stimulation. We found that SnCs not only expressed higher basal levels of various inflammatory cytokines and chemokines as a manifestation of the SASP, but more importantly exhibited hyper-activation of the induction of a variety of inflammatory mediators in response to LPS, IL1β and TNFα stimulation as compared with non-SnCs. This senescence-associated hyper-activation is likely mediated in part via the p38MAPK (p38) and NFκB pathways because LPS stimulation elicited significantly higher levels of p38 phosphorylation and NFκB p65 nuclear translation in SnCs when compared to their non-senescent counterparts and inhibition of these pathways with losmapimod (a p38 specific inhibitor) and BMS-345541 (a selective NFκB inhibitor) attenuated LPS-induced expression of IL6, TNFα, CCL5, and IL1β mRNA in SnCs. These findings suggest that SnCs may play an important role in the age-related increases in the susceptibility to developing an exacerbated inflammatory response and highlight the potential to use senotherapeutics to ameliorate the severity of various devastating inflammatory conditions in the elderly.

Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.203396

DOI - https://doi.org/10.18632/aging.203396

Full text - https://www.aging-us.com/article/203396/text

Correspondence to: Daohong Zhou email: zhoudaohong@cop.ufl.edu

Keywords: cellular senescence, inflammation, SASP, aging

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at http://www.Aging-US.com​​ or connect with us on:

Twitter - https://twitter.com/AgingJrnl
Facebook - https://www.facebook.com/AgingUS/
SoundCloud - https://soundcloud.com/aging-us​
YouTube - https://www.youtube.com/agingus​
LinkedIn -  https://www.linkedin.com/company/aging​

Aging-US is published by Impact Journals, LLC please visit http://www.ImpactJournals.com​​ or connect with @ImpactJrnls

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Top-Performer: Senescent Cells and Inflamm-Aging

Aging-US published this trending review on August 12, 2021, entitled, “Cellular senescence in lymphoid organs and immunosenescence,” by researchers from the Genetics and Genomics Graduate Program, Genetics Institute, College of Medicine, University of Florida, Gainesville, FL; Department of Neuroscience, McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL; Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL.


Abstract:
Immunosenescence is a multi-faceted phenomenon at the root of age-associated immune dysfunction. It can lead to an array of pathological conditions, including but not limited to a decreased capability to surveil and clear senescent cells (SnCs) and cancerous cells, an increased autoimmune responses leading to tissue damage, a reduced ability to tackle pathogens, and a decreased competence to illicit a robust response to vaccination. Cellular senescence is a phenomenon by which oncogene-activated, stressed or damaged cells undergo a stable cell cycle arrest. Failure to efficiently clear SnCs results in their accumulation in an organism as it ages. SnCs actively secrete a myriad of molecules, collectively called senescence-associated secretory phenotype (SASP), which are factors that cause dysfunction in the neighboring tissue. Though both cellular senescence and immunosenescence have been studied extensively and implicated in various pathologies, their relationship has not been greatly explored. In the wake of an ongoing pandemic (COVID-19) that disproportionately affects the elderly, immunosenescence as a function of age has become a topic of great importance. The goal of this review is to explore the role of cellular senescence in age-associated lymphoid organ dysfunction and immunosenescence, and provide a framework to explore therapies to rejuvenate the aged immune system.

Sign up for free Altmetric alerts about this article - https://oncotarget.altmetric.com/details/email_updates?id=10.18632%2Foncotarget.203405

DOI - https://doi.org/10.18632/aging.203405

Full text - https://www.aging-us.com/article/203405/text

Correspondence to: Daohong Zhou email: zhoudaohong@cop.ufl.edu

Keywords: cellular senescence, immunosenescence, immune senescence, senescence associated secretory phenotype (SASP), thymus

About Aging-US

Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.

Please visit our website at http://www.Aging-US.com​​ or connect with us on:

Twitter - https://twitter.com/AgingJrnl​
Facebook - https://www.facebook.com/AgingUS/​
SoundCloud - https://soundcloud.com/aging-us​
YouTube - https://www.youtube.com/agingus​
LinkedIn -  https://www.linkedin.com/company/aging​

Aging-US is published by Impact Journals, LLC please visit http://www.ImpactJournals.com​​ or connect with @ImpactJrnls

Media Contact
18009220957
MEDIA@IMPACTJOURNALS.COM

Top-Performer: Cellular Senescence in Lymphoid Organs and Immunosenescence

Ziath 2D barcode readers

Have you been so frustrated by dodgy equipment that does not work properly in the lab? This poor guy has! He can't get home for tea because his inferior bar-code reader can't determine which wells contain a tube and which wells are empty... If only he had a Ziath Mirage running our new AI-powered empty well detection.

Bad, Bad Scanner, Give me AI! I want AI!

Vlogger Rizwan Chaudhrey interviews Ziath's founder and Managing Director, Neil Benn about sample tracking, why not all tubes are made equal, how to get a great result straight from the freezer and some of the strange things that led to Neil setting up Ziath Ltd 15 years ago.

"Chats with Chaudhrey" - Rizwan talks to Neil Benn, MD of bar-code reader specialist Ziath to see how they got to be market leaders in their field of sample tracking

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