Waters Corporation

Glycan analysis & characterization | Behind the Science

Our host Jennifer Fournier talks with Matt Lauber, Ph.D., a principal applications chemist at Waters, about what's behind released N-glycan analysis. It's highly complicated, protocols are long, but the data is like gold - and understanding glycans is a requirement for biopharma companies. We've heard rumors that a traditional 2 to 3 day protocol can now be done in just 30 minutes. How is it possible? Let's find out.

Behind the Science, episode 1: Just Not Possible 

In episode 1, we learned about a simple, fast way to prepare released N-glycan samples. But are you giving something up for dramatic time savings? Jennifer Fournier talks with Darryl Brousmiche, Ph.D., a synthetic chemist at Waters and one of the creators of RapiFluor-MS, about how the unique labeling reagent tags glycans so that they're visible to optical and mass detectors.

Behind the Science, ep. 2: What's the Catch?

Our host Jen Fournier sets up a face-off between HILIC and RP separations to see which delivers better results. Our chemists Scott McCall and Matt Lauber go head-to-head using glycans. Let's get ready to separate!

Behind the Science, ep. 3: HILIC vs. Reversed-Phase Battle 

Today our host Jennifer Fournier confronts her fear of serial detection. Having a mass assigned to every peak would be nice, but aren't mass spectrometry instruments hard to use? As a chromatography pro who does glycan analysis, Jen has no problem running optical detectors. See what she learns about mass detection with Stephen Koza, a principal applications chemist at Waters.<br/>

Behind the Science, ep. 4: Serial Detection Is Not For Me

Are there better ways to understand what glycans are present and where they are attached to a glycoprotein? Our Behind the Science host Jen Fournier talks with Steve Koza, a principal applications chemist at Waters, about how to determine glycan site occupancy and linkage information.<br/>

Behind the Science, ep. 5: Where's Your Sugar?

It’s time for a glycan therapy session where scientists discuss the “problem children” in the laboratory. Our Behind the Science host Jen Fournier gives scientists coping strategies – and tips and tricks – for difficult glycosylation studies. Topics raised on the therapy couch: problems characterizing the N-glycans of EPO with HILIC and QTof-MS, comparing low-abundant glycan species between an innovator and a biosimilar, and separation of high mannose species critical pairs.

Behind the Science, episode 1: Just Not Possible

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Waters Corporation

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July 28, 2016

Glycan analysis & characterization | Behind the Science

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