Bio-Rad Laboratories

Bio-Rad Laboratories, Inc. is a multinational manufacturer and distributor of life science research products, clinical diagnostics, and analytical instrumentation, based in Hercules, California. The company serves more than 70,000 research and industry customers worldwide through a network of more than 30 wholly-owned subsidiary offices.

Website: http://discover.bio-rad.com

Protein Sample Prep

"Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.

For more tips and tricks on protein electrophoresis and protein sample prepartion, and more, download our Guide to Polyacrylamide Gel Electrophoresis and Detection:
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf

We hope this video addresses your questions; for more information or to suggest topics for future LabNotes episodes, please email LabNotes@Bio-Rad.com. For questions about Bio-Rad products, contact Bio-Rad Technical Support:
http://www.bio-rad.com/en-us/life-science-research/support?WT.mc_id=sm-PQD-WW-electrospotlight-vyt_20150626-nUfxxIeu1yM"

Five Tips for Picking the Right Cell Disruption Method for Protein Analysis

"From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don't have to worry about proteases, we share helpful tips for developing a sample preparation protocol.

For more tips and tricks on protein electrophoresis and protein sample prepartion, and more, download our Guide to Polyacrylamide Gel Electrophoresis and Detection:
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf

We hope this video addresses your questions; for more information or to suggest topics for future LabNotes episodes, please email LabNotes@Bio-Rad.com. For questions about Bio-Rad products, contact Bio-Rad Technical Support:
http://www.bio-rad.com/en-us/life-science-research/support?WT.mc_id=sm-PQD-WW-electrospotlight-vyt_20150626-GLThd7cyvCw"

Four Tips for Developing a Sample Prep Protocol for SDS-PAGE or Chromatography

"In this episode, we share tips and tricks for minimizing protein degradation during cell lysis and sample preparation.

For more tips and tricks on protein electrophoresis and protein sample prepartion, and more, download our Guide to Polyacrylamide Gel Electrophoresis and Detection:
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf

We hope this video addresses your questions; for more information or to suggest topics for future LabNotes episodes, please email LabNotes@Bio-Rad.com. For questions about Bio-Rad products, contact Bio-Rad Technical Support:
http://www.bio-rad.com/en-us/life-science-research/support?WT.mc_id=sm-PQD-WW-electrospotlight-vyt_20150626-yU4el169If0"

Four Tips for Minimizing Protein Degradation During Sample Preparation

"For more info, visit http://www.bio-rad.com/yt/1/MicroRotofor.
This video tutorial demonstrates the disassembly and cleanup of Bio-Rad's MicroRotofor cell following a focusing run. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b30915de-43cb-4b45-ac11-8067e592c8c0&WT.mc_id=yt-lsd-ww-microrotofor-20120912-TlKjMZ0Bpk4"

MicroRotofor™ Tutorial — Cleaning Up

"For more info, visit http://www.bio-rad.com/yt/2/MicroRotofor.
This video tutorial provides an overview of the design and construction of Bio-Rad's MicroRotofor cell. Each component and its function is explained in detail; basic assembly is demonstrated, and the included accessories are shown. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b30915de-43cb-4b45-ac11-8067e592c8c0&WT.mc_id=yt-lsd-ww-microrotofor-20120912-3Nsrg3a5PhM"

MicroRotofor™ Tutorial — Components

"For more info, visit http://www.bio-rad.com/yt/3/MicroRotofor.
This video tutorial demonstrates fraction collection from Bio-Rad's MicroRotofor cell using the integrated harvesting tray/vacuum aspiration assembly. Full separation by isoelectric point (pI) is visually confirmed by observation of the migration of the colored protein standards. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on pI. Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b30915de-43cb-4b45-ac11-8067e592c8c0&WT.mc_id=yt-lsd-ww-microrotofor-20120912-ngJkRiwXcHA"

MicroRotofor™ Tutorial — Harvesting

"For more info, visit http://www.bio-rad.com/yt/4/MicroRotofor.
This video tutorial demonstrates sample loading in the focusing chamber of Bio-Rad's MicroRotofor cell. The narrator also shows how to remove air bubbles from the chamber. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b30915de-43cb-4b45-ac11-8067e592c8c0&WT.mc_id=yt-lsd-ww-microrotofor-20120912-N3BrUKXHMT4"

MicroRotofor™ Tutorial — Loading the Sample

"For more info, visit http://www.bio-rad.com/yt/5/MicroRotofor.
This video tutorial demonstrates the loading and operation of Bio-Rad's MicroRotofor cell for a focusing run, starting with a previously assembled focusing chamber and electrodes. Following isoelectric focusing, Bio-Rad's colored protein standards provide a visual reference for the degree of separation. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b30915de-43cb-4b45-ac11-8067e592c8c0&WT.mc_id=yt-lsd-ww-microrotofor-20120912-2iAoXgEz6Sw"

MicroRotofor™ Tutorial — Performing the Focusing Run

"For more info, visit http://www.bio-rad.com/yt/6/MicroRotofor.
This video tutorial demonstrates how to assemble the focusing chamber and electrodes of Bio-Rad's MicroRotofor cell into an integrated focusing assembly. The MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b30915de-43cb-4b45-ac11-8067e592c8c0&WT.mc_id=yt-lsd-ww-microrotofor-20120912-eS3syan16a4"

MicroRotofor™ Tutorial — Preparing the Focusing Assembly

"For more info, visit http://www.bio-rad.com/yt/1/Rotofor.
This video tutorial demonstrates the assembly of the electrodes in Bio-Rad's Rotofor cell in full detail. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small to medium volumes (3–60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-kwRAxIdOTrg"

Rotofor® Tutorial — Assembling Electrodes

"For more info, visit http://www.bio-rad.com/yt/2/Rotofor.
This video tutorial demonstrates the assembly of the Rotofor focusing chamber and electrodes on the cooling finger and correct placement on the chassis of the Rotofor cell, including alignment of the chamber ports with the membrane core. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small to medium volumes (3–60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-_lr7U613UxE"

Rotofor® Tutorial — Assembling the Focusing Chamber and Electrodes

"For more info, visit http://www.bio-rad.com/yt/3/Rotofor.
This video tutorial demonstrates fraction collection from the Rotofor cell using the included vacuum aspiration assembly. Following isoelectric focusing, Bio-Rad's colored protein standards provide a visual reference for the degree of separation. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of  the presence of highly abundant proteins such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small to medium volumes (3–60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-cJd7PJsNSDI"

Rotofor® Tutorial — Collecting Fractions

"For more info, visit http://www.bio-rad.com/yt/4/Rotofor.
This video tutorial provides an overview of the design and construction of the electrodes for Bio-Rad's Rotofor cell along with guidelines for their storage and use. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small to medium volumes (3-60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-7a8qUI7ED3c"

Rotofor® Tutorial — Electrodes Overview

"For more info, visit http://www.bio-rad.com/yt/5/Rotofor.
This video tutorial provides an overview of the design, construction, and assembly of the standard focusing chamber for Bio-Rad's Rotofor cell. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small to medium volumes (3-60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-VGH15jS3umI"

Rotofor® Tutorial — Focusing Chamber Overview

"For more info, visit http://www.bio-rad.com/yt/6/Rotofor.
This video tutorial demonstrates sample loading and operation of Bio-Rad's Rotofor cell for a focusing run, starting with a previously assembled focusing chamber and electrodes. The narrator also shows how to remove air bubbles from the chamber and set the power supply. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant protein such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small-medium volumes (3-60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.

http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-4eyMz3dOmmQ"

Rotofor® Tutorial — Loading and Running Sample

"For more info, visit http://www.bio-rad.com/yt/7/Rotofor.
This video tutorial provides an overview of the design and construction of Bio-Rad's Rotofor cell. Each component and its function is explained in detail; basic assembly is demonstrated, and the included accessories are shown. The Rotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Due to the great complexity of samples such as tissues, cells, and body fluids, protein researchers may want to fractionate crude samples prior to analysis by two-dimensional (2-D) gel electrophoresis. Fractionation is particularly relevant when trying to detect low-abundance proteins in samples like serum, cerebrospinal fluid (CSF), and urine because of the presence of highly abundant proteins such as albumin and immunoglobulins. The Rotofor and mini Rotofor cells use liquid-phase IEF to fractionate small to medium volumes (3–60 ml) of proteins in free solution based on isoelectric point (pI). Protein fractionation by pI improves sample loading and separation on narrow- and micro-range IPG strips in downstream 2-D electrophoresis applications.


http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=684c3b20-b5b6-4ada-a45a-820a9db12763&WT.mc_id=yt-lsd-ww-rotofor-20120912-UQdmEwYImY0"

Rotofor® Tutorial — Product Overview

"For more info, visit http://www.bio-rad.com/yt/SureBeads
Bio-Rad’s SureBead Magnetic Beads System provides faster and more reliable immunoprecipitation of proteins and protein complexes at a price comparable to leading agarose beads. The SureBeads System consists of protein A- or protein G-coupled magnetic beads and an ergonomically designed tube rack with high-strength magnets that separate SureBeads Magentic Beads and pulled-down proteins from the supernatant in seconds.

The SureBeads Magnetic Beads System makes immunoprecipitation:
Faster – eliminates time-consuming centrifugation steps
Easier — simplifies pipetting by immobilizing beads against the side wall of tubes instead of the bottom
Reliable — ensures accurate and reproducible results through more consistent IgG binding capacity
Affordable — unique surface chemistry allows you to use less antibody more efficiently

http://www.bio-rad.com/en-us/product/protein-g-magnetic-beads?WT.mc_id=yt-lsd-ww-surebeads-20140822-aHsBID5oS4k"

Five Tips for Picking the Right Cell Disruption Method for Protein Analysis

Posted by

Bio-Rad Laboratories

721 views

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March 14, 2017

Protein Sample Prep

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