Advanced Topics in Cell Sorting

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Bio-Rad Laboratories

"For more info, visit http://www.bio-rad.com/yt/4/S3 In this webinar, Dan Fox of Propel Labs explores the principles of cell sorting including the theory of droplet cell sorting, how to assess cell sorter performance, and how sorting speed influences results. Dan also discusses the S3™ Cell Sorter’s unique sorting modes, the sort mode process and logic, and how the S3 Sorter simplifies the science of cell sorting. The first truly walk-up cell sorter, the S3 delivers an easy-to-use, affordable solution for busy scientists. The S3 Cell Sorter is an automated benchtop cell sorter featuring either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors. The S3 Cell Sorter is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry or cells bearing fluorescent markers such as labeled antibodies. Features and Benefits • Simple instrument setup — automated drop delay calculation and droplet break-off monitoring enables precise 1–4 color sorting with minimal training • Compact footprint — only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system • Reduced carryover — dual-position loading stage ensures the sample line remains clean between sorting runs • 2-way cell sorting — sort two different defined populations at the same time • High speed and high purity — sort cells fast while maintaining high sensitivity and purity • Intuitive interface — user-friendly ProSort™ software enables effortless instrument control and sort logic definition Presenter: Dan Fox Director of Research and Development Propel Labs, Fort Collins, Colorado Dan Fox has over a decade of experience in designing high-speed cell sorters, and holds numerous patents on flow cytometry and sorting technology. Dan is one of the principal designers who developed Bio-Rad’s S3™ Cell Sorter. http://www.bio-rad.com/en-us/category/flow-cytometry?WT.mc_id=yt-GXD-ww-s3-20141006-N-kHOL6sJdM"