METTLER TOLEDO Pipetting Solutions

Good Pipetting Techniques

Maintaining consistency during aspiration can improve accuracy by up to 5%. To ensure that you are achieving the highest accuracy, use consistent pipetting rhythm from sample to sample as shown here. Learn more: http://www.mt.com/gpp-tips

Aspiration - Good Pipetting Technique

Improve your pipetting accuracy by up to 1% with good dispensing technique. For the highest consistency, touch the vessel wall with the tip while dispensing. Then, touch off by sliding the tip up the wall to remove any liquid clinging to the orifice. Learn more: http://www.mt.com/gpp-tips http://www.mt.com/gpp

Dispensing - Good Pipetting Technique 

Immersing the tip to the correct depth will improve your accuracy by up to 5%. Immersing the tip too deeply can cause too much liquid to be aspirated and create bubbles. Conversely, positioning the tip too close to the surface can aspirate air. Learn more: www.mt.com/gpp-tips www.mt.com/gpp

Pipette Tip Immersion - Good Pipetting Technique 

Pre-rinsing is a fast, easy way to increase accuracy by up to 0.2%. Why? It helps neutralize the capillary effect in micro-volume pipettes, and for larger volume tips, equalizes the temperature of the air inside the tip with the temperature of the sample. Learn more: http://www.mt.com/gpp-tips

Pre-rinsing Pipette Tips - Good Pipetting Technique 

Pipetting with an air-displacement pipette is a five step process that is often referred to as "forward pipetting." In this short video, we demonstrate the five distinct steps of pipetting and how to do them correctly. Learn more: http://www.mt.com/gpp-tips

The Pipetting Cycle - Good Pipetting Technique 

When setting the micrometer on a pipette, always "dial down" to the desired volume. By doing do, you ensure better accuracy by reducing the effects of mechanical backlash or slippage within the micrometer's gears.

Learn more: http://www.mt.com/gpp-tips

Aspiration - Good Pipetting Technique

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METTLER TOLEDO Pipetting Solutions

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December 10, 2014

Good Pipetting Techniques

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