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Subcellular fractionation and enrichment strategies for improved mass spectrometry analysis

from Technology Networks Webinars

Fractionation or simplification of a complex sample before mass spec analysis helps bring many of these lower copy number proteins into view, helping researchers better understand changes in their expression, cellular location, post-translational modifications, and some of the interacting proteins that may influence their biological function. Fractionation can be accomplished on multiple levels, ranging from very broad subcellular compartmentalization to targeted immunoselection. In this webinar, you will learn how: - Differential detergent subcellular fractionation can improve protein identification and identify co-localizing proteins. - Enrichment of integral and peripheral membrane proteins is accomplished using a non-denaturing differential detergent solubilization protocol. - Immunoprecipitation facilitates the identification of proteins that otherwise cannot be detected in whole cell lysates, and how IP in combination with SILAC facilitates monitoring changes in protein expression, post-translational modifications, and helps to identify potential interacting proteins. For Research Use Only. Not for Use in Diagnostic Procedures. To register for this on demand webinar please copy and paste the below link into your browser:

about 3 years ago

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Technology Networks Webinars
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