Bio-Rad Laboratories, Inc. is a multinational manufacturer and distributor of life science research products, clinical diagnostics, and analytical instrumentation, based in Hercules, California. The company serves more than 70,000 research and industry customers worldwide through a network of more than 30 wholly-owned subsidiary offices.

Website: http://discover.bio-rad.com

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Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.

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©2021 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions.  All trademarks used herein are the property of their respective owner.

Quick Tips: How To Prepare the Bio-Plex System for Optimal Data Acquisition

Watch this video for a step-by-step tutorial on how to properly set up a Trans-Blot Cell blotting sandwich for efficient polyacrylamide gel transfer during western blotting.

Learn more at https://www.bio-rad.com/QuickTips

We Are Bio-Rad.

Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.

Connect with Bio-Rad Online:
Website: http://www.bio-rad.com/ 
LinkedIn: https://www.linkedin.com/company/1613...  
Facebook: https://www.facebook.com/biorad/ 
Twitter: https://twitter.com/BioRadLifeSci 
Instagram: @BioRadLabs
Snapchat: @BioRadLabs

©2021 Bio-Rad is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions.  All trademarks used herein are the property of their respective owner.

Quick Tips: How to Setup a Mini Trans-Blot Cell for Western Blot Transfer

Uneven sample migration in polyacrylamide gel electrophoresis (PAGE), also called smiling and frowning, can make it difficult to accurately estimate band size and differentiate between bands on your western blot. Watch this video to learn about common causes of uneven sample migration. These tips will help ensure that you get the best possible resolution in your western blotting experiments.

Learn more at https://www.bio-rad.com/QuickTips

We Are Bio-Rad.

Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.

Connect with Bio-Rad Online:
Website: http://www.bio-rad.com/ 
LinkedIn: https://www.linkedin.com/company/1613...  
Facebook: https://www.facebook.com/biorad/ 
Twitter: https://twitter.com/BioRadLifeSci 
Instagram: @BioRadLabs
Snapchat: @BioRadLabs


Quick Tips: How to Minimize Band Distortion During Gel Electrophoresis for Western Blotting

Watch this video to learn more about targeting a specific area on a western blot for auto exposure in Image Lab Touch Software. This is a critical feature for western blots that contain unspecified signal sources that are much brighter than your bands of interest.  

Learn more at https://www.bio-rad.com/quicktips

Quick Tips: Targeting Area of Interest for Auto Exposure in Image Lab Touch Software

This video will demonstrate how to quickly and effectively evaluate your western blot transfer using Stain-Free imaging technology with a Bio-Rad imaging system. 

Be able to quickly visualize blots and gels immediately after electrophoresis and post transfer without the need to perform any staining steps. This will help you save time and provide confirmation that your protein samples made it onto the membrane efficiently.  

Learn more at https://www.bio-rad.com/quicktips

Quick Tips: Evaluating Western Transfers using Stain-Free Gels and Bio-Rad Imaging System

Watch this video to explore considerations for optimizing incubation time and dilution of primary antibodies when performing a western blot.

Commercially purchased antibodies should have a starting dilution recommended by the manufacturer. Plan your dilution series around the manufacturer-recommended dilution. This should help establish the optimal dilution for your specific target protein.

Once the optimal primary antibody dilution has been established, you can optimize incubation time. The typical recommendation for primary antibody incubation is 1 to 2 hours at room temperature or overnight at 4 degrees Celsius. The optimal choice depends on your antibody and sample.

However, a longer incubation time may result in the higher background and non-specific binding. These issues should be minimal if the primary antibody dilution has already been optimized.

Learn more at https://www.bio-rad.com/quicktips

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