Webinars brought to you by Technology Networks

Webinars by Technology Networks 

Many viruses are surrounded by lipid membranes, including SARS-CoV-2 and influenza. Both viruses contain viroporins, which are small, single pass transmembrane proteins that oligomerize to form ion channels. The influenza M2 viroporin is already a clinically approved antiviral drug target. In other coronaviruses, the E protein viroporin which is involved in growth, could be a potential target for COVID-19 vaccine and antiviral development. Although the oligomeric state of the M2 and E proteins are thought to be tetrameric and pentameric respectively, determining the oligomeric states of small transmembrane peptide complexes within membranes is challenging.

Native or non-denaturing mass spectrometry (MS) data suggests a more complex picture of the oligomeric state than previously assumed, highlighting the need for novel analytical methods to understand how these important biomedical targets interact within lipid membranes. This webinar will introduce the native MS and lipid nanodisc techniques and demonstrate how this can be used to directly measure the oligomeric state of protein and peptide complexes within intact model lipid bilayers. Ongoing studies on the M2 and E protein will be discussed.

Native Mass Spectrometry and COVID-19: Uncovering Drug Binding and Oligomerization

To confidently characterize complex lipidomes of whole organisms, researchers require comprehensive MS/MS coverage of detected lipids. Compared to conventional analyses, the timsTOF Pro, used with Parallel Accumulation Serial Fragmentation (PASEF) acquisition mode, acquires MS/MS spectra with uncompromised sensitivity and resolution.

This webinar will demonstrate Bruker’s fully integrated lipidomics workflow in MetaboScape and outline how novel 4D-lipidomics tools has been used to gain a better understanding of how a sphingolipid mutation in the C. elegans relates to an increased lifespan – or in other words, the fountain of youth.

4D-Lipidomics™: Searching for the Fountain of Youth

Mucosal barriers are the gateways to all internal organs, serving to transport oxygen, nutrients, and waste and at the same time performing enormous feats of protection against infection and other hazardous insults. The explosion of interest in the human microbiome – especially but not only that in the gut – has driven new interest in building human mucosal barrier models.

This talk will highlight three related themes: (i) engineering synthetic microenvironments to expand primary adult epithelial organoids and induce morphogenesis into mucosal barriers (ii) engineering microfluidic devices to create microbial-mucosal interfaces that enable chronic co-culture of the most super strict anaerobes, such as Faecalibacterium prausnitzii, with a colon mucosal barrier and (iii) interconnection of mucosal barriers with other tissues in systemic circuits to illuminate the role of gut-derived bacterial metabolites on function of other organ systems. Examples will emphasize how these approaches can be used to model chronic inflammatory diseases.

Engineering Mucosal Barriers: From Organoids to Organs-on-Chips

CAR T-cell therapies are emerging anti-cancer treatments, whereby the patient’s immune cells are engineered to express chimeric antigen receptors (CARs) on their surface. Wider adoption of this therapeutic modality would benefit from innovative technologies that can improve the characterization of raw material quality attributes (MQA) and final product quality attributes (PQA).

Here, we present the development of a sensitive proteomic method to characterize CAR T cells using SWATH™ technology. We evaluated key steps of the data acquisition, from separation to detection, to enhance the sensitivity by data dependent and independent methods.

The data represent an instrument benchmarking that will ultimately be used as performance qualification metrics to assure instrument control for biologically relevant decision making of CAR T proteomic samples.

Development of a Sensitive SWATH™ MS Workflow To Advance the Characterization of CAR T-Cell Therapies

Metastatic disease is the leading cause of mortality in patients with solid tumors. Despite decades of research, we only partially understand the underlying cellular and molecular mechanisms.

This gap in knowledge represents a significant barrier for the prevention and treatment of metastasis. In part, a lack of relevant accessible model systems capable of capturing the complexities of metastatic disease progression has hindered our progress. As a result, researchers are turning towards tissue engineered ex vivo biomimetic microphysiological systems.

In this webinar, we discuss how to use these systems to model metastatic progression, evaluate therapeutics and improve our understanding of the bidirectional crosstalk that occurs between host tissue cells and the metastatic cancer cells.

A Microphysiological Model of Metastatic Progression

Current in vitro approaches for investigational toxicology, drug metabolism (DMPK) and safety are limited and are not fully representative of human response; therefore, drugs showing acceptable preclinical toxicity often fail in human clinical trials, and accurately predicting human drug exposure for new compounds is challenging and costly.

Microphysiological Systems (MPS), or Organ-on-chip (OOC) technologies are a trending way to bridge the gap between traditional DMPK/toxicology assays and human trials by providing more robust and reliable data that translate into clinical outcomes.

CN Bio offers preclinical investigational toxicology and DMPK services using its proprietary, industry-proven PhysioMimix™ OOC technology and liver-on-a-chip model. In this webinar, we will share our latest advances in investigational toxicology, drug clearance and drug safety, highlighting how the translatable, human-relevant data generated using CN Bio’s liver-on-a-chip model can be used to support (or call into question) the conclusions of internal pre-clinical studies, helping you to make data driven decisions with greater confidence.

Webinars by Technology Networks

Home

Videos

Playlists

About

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.