Teach Me in 10 guests explore a variety of topics, from the latest developments in gene editing technology, neuroscience and cancer, to writing a scientific paper or applying for your first academic position. We invite you to learn from their insights and engage with the videos as you learn from the experts.

Teach Me in 10

As the global biopharmaceutical industry continues to grow, there is a need for innovative solutions that accelerate development and manufacturing workflows. Label-free protein analysis offers unique advantages for characterizing the biomolecular interaction kinetics of therapeutic candidates during this process. The Sartorius Octet® Label-Free Platform has become an industry leader for its bio-layer interferometry (BLI)-based systems for label-free, real-time analysis.

Recently, the platform has expanded to include the next-generation surface plasmon resonance (SPR)-based instrument, Octet® SF3, making Sartorius the only company to offer both label-free technologies to its customers.

In this episode of Teach Me in 10, we speak with Helge Schnerr, global campaign manager at Sartorius, about the motivation behind this move, the game-changing features on the SF3 and how it all translates to empowering scientists.

For more information, visit https://www.sartorius.com/octet-SPR.

Powered and Prepared for Your Next Label-Free Challenge With Dr. Helge Schnerr

Research by a team of neuroscientists from the University of Nevada, Las Vegas (UNVL) has shown that it is possible to identify the molecular mechanisms underlying a host of adverse symptoms associated with unique subtypes of neurodevelopmental disorders. This work could one day improve the lives of millions of people worldwide.
Intellectual disability (ID) is a common neurodevelopmental disorder that can arise from genetic mutations ranging from trisomy to single nucleotide polymorphism. Trisomy of the 21st chromosome (Down syndrome) is the most common underlying cause of ID, but mutations in a growing number of single genes have been identified as causative in ID, including NONO and ARHGEF9. 

In this episode of Teach Me in 10, UNLV neuroscientist Dr. Rochelle Hines joins us to discuss the team’s latest findings, which show that mutations in ARHGEF9  — the gene that codes for collybistin — lead to ID through impaired α2 subunit function.

Want to learn more? Rochelle suggests you check out the following resources:  

https://www.nature.com/articles/s41380-022-01468-z
https://www.nature.com/articles/s41467-018-05481-1

Neurodevelopmental Disorders With Rochelle Hines

Next-generation sequencing (NGS) target enrichment methods provide solutions to focus on and sequence a small portion of the human genome. This saves sequencing and analysis costs for labs that study genetic diseases. These savings typically range from approximately 100 to 100,000 fold, depending on the application and the amount of the genome being targeted. Until now, there have been two primary target enrichment methods used in the field.
 Hybridization capture methods are considered high-performance solutions that tend to require cumbersome steps and an overnight hybridization incubation for optimal performance. On the other hand, amplicon technologies are considered fast and easy but generally do not have the same level of performance compared to capture technologies. Primer Extension Target Enrichment (PETE) is a newly developed technology that uses DNA polymerase extension reactions to provide a high-performance target enrichment solution that is easy to use and can be performed in a single day. 

In this Teach Me in 10, Brian Godwin, director of reagent and assay development at Roche sequencing, joins Lucy Lawrence to give us an overview of how each of the three target enrichment technologies work along. Godwin also shares a description of common target enrichment performance metrics.

Introducing Primer Extension Target Enrichment for NGS With Brian Godwin

Next Generation Sequencing (NGS) target enrichment methods provide solutions to focus on and sequence a small portion of the human genome.  This saves sequencing and analysis costs for labs that study genetic diseases.  These savings typically range from approximately 100 to 100,000 fold depending on the application and the amount of the genome being targeted. 

Until now, there have been two primary target enrichment methods used in the field.  Hybridization capture methods are considered high-performance solutions that tend to require cumbersome steps and an overnight hybridization incubation for optimal performance.  On the other hand, amplicon technologies are considered fast and easy but generally do not have the same level of performance compared to capture technologies.

In this episode of Teach Me in 10, Lucy Lawrence is joined by Brian Godwin the Director of Reagent and Assay Development at Roche Sequencing and he shares methods, performance metrics and application data surrounding NGS Target Enrichment. 

Introducing Primer Extension Target Enrichment for NGS with Brian Godwin 

Harvesting your cells is an important step in maintaining healthy cultures.  Good technique will lead to a healthy single-cell suspension.  There are many harvesting options depending on the cell line and application.  In this video, we will discuss some tips and tricks for harvesting cells.

Techniques for Harvesting Adherent Cells with Hilary Sherman

In this episode of Teach Me in 10, Lucy Lawrence is joined by the Global Product Manager of Applications and Flow Reagents at Bio-Rad Laboratories, Dr. Chris Brampton.

Chris will cover the basics of antibodies, the reasons why you need flow cytometry–specific antibodies and some basic best practices to achieve improved data and increase the likelihood of a successful outcome for flow cytometry experiments.

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