Creative Proteomics videos

Proteomics studies play an increasing role in the field of biology. The use of mass spectrometry (MS) in combination with a range of separation methods is the main principal methodology for proteomics. The two principal approaches to identifying and characterizing proteins using MS are the “bottom-up”, which analyze peptides by proteolytic digestion, and “top-down”, which analyze intact proteins.  More at https://www.creative-proteomics.com/

Bottom-up  Proteomics and Top-down Proteomics

Proteins, biomolecules or macromolecules, perform a wide range of functions in organisms. Almost all of the cellular processes require that proteins specifically recognize a multitude of different interaction partners. They can carry out their roles by interacting with other molecules, including DNA, RNA, proteins and small molecules. Protein and protein interactions (PPIs), which refers to intentional physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces. https://www.creative-proteomics.com/Services/Co-immunoprecipitation-co-IP.htm

Brief Introduction to Protein-Protein Interaction (PPI)

Protein-protein interactions (PPIs) are the basis of the cellular structure and functions. It has been estimated that more than 80% of proteins do not operate alone but in complexes. The analysis of PPIs is able to help us understand cellular organization, process, and functions. PPIs analysis may discovery unique and unforeseen functional roles for well-known proteins. And it can discover previously unknown proteins by association knowns proteins. https://www.creative-proteomics.com/

PPI Methods

MS is widely used in free oligosaccharides, glycosaminoglycans as well as the glycan portions of glycoproteins, proteoglycans, and glycolipids. And there is a wide range of MS techniques are available for glycomics analysis. As research continues on MS-based glycomics analysis, we believe that clear understanding the mechanisms of important biological processes will be achieved in the near future. https://www.creative-proteomics.com/

Glycomics Analysis by Mass Spectrometry

Glycans are defined as the compounds that consist of a large number of monosaccharides linked glycosidically. Glycans have diverse biological functions and it can be classified into two broad categories. One is the structural and modulatory properties of glycans and the other is the specific recognition of glycans by other molecules, most commonly, glycan-binding proteins (GBPs). The GBPs can be divided into two types, intrinsic GBPs and extrinsic GBPs. The intrinsic GBPs recognize glycans from the same organism and mediate cell-cell interactions or recognize extracellular molecules. The extrinsic GBPs which contain pathogenic microbial adhesins, agglutinins, or toxins, recognize glycans from different organisms and some also mediate symbiotic relationships.

Brief Introduction of Glycan Microarrays

Creative Proteomics has established a highly sensitive HPLC-MS/MS pipeline that can analyze N- and O-methylation in both eukaryotic and prokaryotic organisms. In addition, we have optimized our protocol, to enable more fast and sensitive site mapping service for methylation analysis.

https://www.creative-proteomics.com/services/Methylation.htm

Creative Proteomics videos

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