Creative Proteomics videos

Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method based on mass spectrometry that identifies and quantifies relative differential changes in protein abundance. First used in quantitative proteomics in 2002, it provides accurate relative quantification without any chemical derivatization or manipulation.

https://www.creative-proteomics.com/services/silac-based-proteomics-analysis.htm

Stable Isotope Labeling

iTRAQ (Isobaric tag for relative and absolute quantitation) was developed by Applied Biosystems Incorporation in 2004. iTRAQ is a isobaric labeling method to determine the amount of proteins from different sources in just one single experiment. The iTRAQ reagents available always are set of 4-plex and 8-plex mass tages that can be used to label and et quantitative information on up to 4 and 8 different samples in one experiment
https://www.creative-proteomics.com/services/itraq-based-proteomics-analysis.htm

iTRAQ-based Proteomics Approaches

Glycan microarray has become a sensitive and versatile approach for the analysis of glycosylation changes in complex biological samples. It can not only investigate glycan-GBPs binding but also provide kinetic analysis of glycan–protein interactions. Nowadays, this application has rapidly expanded and now achieves successful discrimination of selective interactions between glycans and, not only proteins, but also viruses, bacteria and eukaryotic cells, and most recently even live cell responses to immobilized glycans. Glycan microarray can be used to discover functional glycans, characterize glycan-processing enzymes, and detect pathogens for diagnosis. It is also suitable for monitoring multiple molecular interactions with biomolecules where glycans are involved, offering a large variety of bioassay options.

As one of the leading companies in the omics field with over years of experience in omics study, Creative Proteomics provides Glycan Microarray Assay customized to your needs. https://www.creative-proteomics.com/services/glycan-microarray-assay.htm

Glycan Microarray Assay

Proteomics is a large-scale comprehensive study of a specific proteome, including information on protein abundances, the variations and modifications, as well as their interacting partners and networks. In discovery proteomics, proteome analysis can be performed in two different strategies, bottom-up and top-down approaches, respectively. In the bottom-up approach, a crude protein mixture undergoes protease digestion first, and then separation by liquid chromatography, followed by MS analysis. In the top-down method, proteins are characterized by MS without prior proteolysis. This type of approach has the advantage of providing greater sequence coverage, resolution of sequence ambiguities and preservation of PTMs. https://www.creative-proteomics.com/services/proteomics-service.htm

Protein Sequencing

Proteins are the critical players in molecular recognition at the core of all biological processes. They can interact with the other components of cells, such as small-molecule metabolites, nucleic acids, membranes, and other proteins to build supramolecular assemblies and elaborate molecular machines that perform all sorts of functions, from chemical catalysis and mechanical work to signaling and regulation. As one of the most important interactions, Protein-Protein Interactions have been studied widely. So far large scale protein-protein interactions have been identified, and all the generated data collected together in specialized databases, enables the creation of large protein interaction networks. https://www.creative-proteomics.com/services/protein-protein-interactions-analysis-service.htm

Techniques for Protein-Protein Interaction

Equipped with advanced tandem mass spectrometry for determination of lipid composition, powerful bioinformatic analytic systems, and a panel of experienced scientists and technicians, Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.
https://www.creative-proteomics.com/services/targeted-lipidomics.htm

Creative Proteomics videos

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