Creative Proteomics videos

Proteins, biomolecules or macromolecules, perform a wide range of functions in organisms. Almost all of the cellular processes require that proteins specifically recognize a multitude of different interaction partners. They can carry out their roles by interacting with other molecules, including DNA, RNA, proteins and small molecules. Protein and protein interactions (PPIs), which refers to intentional physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces. https://www.creative-proteomics.com/Services/Co-immunoprecipitation-co-IP.htm

Brief Introduction to Protein-Protein Interaction (PPI)

Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method based on mass spectrometry that identifies and quantifies relative differential changes in protein abundance. First used in quantitative proteomics in 2002, it provides accurate relative quantification without any chemical derivatization or manipulation.

https://www.creative-proteomics.com/services/silac-based-proteomics-analysis.htm

Stable Isotope Labeling

iTRAQ (Isobaric tag for relative and absolute quantitation) was developed by Applied Biosystems Incorporation in 2004. iTRAQ is a isobaric labeling method to determine the amount of proteins from different sources in just one single experiment. The iTRAQ reagents available always are set of 4-plex and 8-plex mass tages that can be used to label and et quantitative information on up to 4 and 8 different samples in one experiment
https://www.creative-proteomics.com/services/itraq-based-proteomics-analysis.htm

iTRAQ-based Proteomics Approaches

Proteomics studies play an increasing role in the field of biology. The use of mass spectrometry (MS) in combination with a range of separation methods is the main principal methodology for proteomics. The two principal approaches to identifying and characterizing proteins using MS are the “bottom-up”, which analyze peptides by proteolytic digestion, and “top-down”, which analyze intact proteins.  https://www.creative-proteomics.com/resource/top-down-proteomics-vs-bottom-up-proteomics.htm

Bottom-up Proteomics and Top-down Proteomics at Creative Proteomics

SILAC-based quantitative proteomics (SILAQ) is an innovative technology used in high throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions. https://www.creative-proteomics.com/Services/SILAC-based-proteomics-analysis.htm

SILAQ

Creative Proteomics has established a highly sensitive HPLC-MS/MS pipeline that can analyze N- and O-methylation in both eukaryotic and prokaryotic organisms. In addition, we have optimized our protocol, to enable more fast and sensitive site mapping service for methylation analysis.

https://www.creative-proteomics.com/services/Methylation.htm

Creative Proteomics videos

Home

Videos

Playlists

About

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.