Creative Proteomics videos

Acetylation refers to a reaction that introduces an acetyl functional group into a chemical compound, in which the hydrogen atom of a hydroxyl group is replaced by an acetyl group (CH3CO) to yield a specific ester, the acetate. Protein acetylation commonly has two different forms. In humans, almost (80%-90%) proteins become co-translationally acetylated at their Nα-termini of the nascent polypeptide chains. Another type is typically acetylated on lysine residues. More can be found at https://www.creative-proteomics.com/

Brief Introduction of Protein Acetylation

Protein phosphorylation, a reversible process, is characterized by adding phosphate donated from ATP and removing phosphate from a phosphorylated protein substrate. And the protein phosphorylation is catalyzed by protein kinase and phosphatase (PP). In eukaryotic cells, about 86.4% of the protein phosphorylation events occur on serine (Ser or S), while 11.8% protein phosphorylation events occur on threonine(Thr or T) whereas only 1.8% on tyrosine (Tyr or Y) residues.  https://www.creative-proteomics.com/

Phosphorylation analysis by mass spectrometry

Peptide Mass Fingerprinting (PMF), also known as mass fingerprinting, was developed in 1993. It is a high throughput protein identification technique in which the mass of an unknown protein can be determined. PMF is always performed with Matrix-assisted laser/desorption ionization time of flight (MALDI-TOF) mass spectrometry. Learn more at https://www.creative-proteomics.com/resource/the-principle-characteristics-and-application-of-peptide-mass-fingerprinting.htm

Protein identification-Peptide mass fingerprinting (PMF)

SILAC-based quantitative proteomics (SILAQ) is an innovative technology used in high throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions. https://www.creative-proteomics.com/Services/SILAC-based-proteomics-analysis.htm

SILAQ

Creative Proteomics has established a highly sensitive HPLC-MS/MS pipeline that can analyze N- and O-methylation in both eukaryotic and prokaryotic organisms. In addition, we have optimized our protocol, to enable more fast and sensitive site mapping service for methylation analysis.

https://www.creative-proteomics.com/services/Methylation.htm

Protein Methylation Analysis

Protein-protein interactions (PPIs) are the basis of the cellular structure and functions. It has been estimated that more than 80% of proteins do not operate alone but in complexes. The analysis of PPIs is able to help us understand cellular organization, process, and functions. PPIs analysis may discovery unique and unforeseen functional roles for well-known proteins. And it can discover previously unknown proteins by association knowns proteins. https://www.creative-proteomics.com/

Creative Proteomics videos

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