Creative Proteomics videos

Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method based on mass spectrometry that identifies and quantifies relative differential changes in protein abundance. First used in quantitative proteomics in 2002, it provides accurate relative quantification without any chemical derivatization or manipulation.

https://www.creative-proteomics.com/

Stable Isotope Labeling

Label-free protein quantification is a mass spectrometry-based method for identifying and quantifying relative changes in two or more biological samples instead of using a stable isotope-containing compound to label proteins. There is no limit on the number of samples in label-free protein quantification and it’s in principle applicable to any kind of sample, including materials that cannot be directly metabolically. It is ideal for large-sample analyses in clinical screening or biomarker discovery experiments.   https://www.creative-proteomics.com/

Label-free Protein Quantification

iTRAQ (Isobaric tag for relative and absolute quantitation) was developed by Applied Biosystems Incorporation in 2004. iTRAQ is a isobaric labeling method to determine the amount of proteins from different sources in just one single experiment. The iTRAQ reagents available always are set of 4-plex and 8-plex mass tages that can be used to label and et quantitative information on up to 4 and 8 different samples in one experiment
https://www.creative-proteomics.com/

iTRAQ-based Proteomics Approaches

Equipped with advanced tandem mass spectrometry for determination of lipid composition, powerful bioinformatic analytic systems, and a panel of experienced scientists and technicians, Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.
https://www.creative-proteomics.com/services/targeted-lipidomics.htm

Mass Spectrometry Based Lipidomics at Creative Proteomics

Creative Proteomics has established a highly sensitive HPLC-MS/MS pipeline that can analyze N- and O-methylation in both eukaryotic and prokaryotic organisms. In addition, we have optimized our protocol, to enable more fast and sensitive site mapping service for methylation analysis.

https://www.creative-proteomics.com/services/Methylation.htm

Protein Methylation Analysis

SILAC-based quantitative proteomics (SILAQ) is an innovative technology used in high throughput quantitative analysis of large protein complexes, protein-protein and protein-small molecule interactions. https://www.creative-proteomics.com/Services/SILAC-based-proteomics-analysis.htm

Creative Proteomics videos

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