Bioarrayy

Creative Proteomics provides Label-free methods for both relative and absolute quantification, which a rapid and low-cost alternative to other quantitative proteomic approaches.

Label-free quantification
 is a method in mass spectrometry that aims to determine the relative amount of proteins in two or more biological samples. Unlike other methods for protein quantification, label-free quantification does not use a stable isotope containing compound to chemically bind to and thus label the protein.

Label-free quantification

The sequence of peptide/protein is important to study the biological function of the peptide/protein. However, complete characterization of peptides/proteins, including post-translational modifications (PTMs), sequence mutations and variants, is very challenging. There are two approaches to determine the sequence of peptide/protein by mass spectrometry: database search and de novo sequencing. Database search approach compares acquired mass spectra to a database of known protein sequences to identify the protein sequences. de-novo protein sequence analysis is a process in which amino acid sequences are directly interpreted from tandem mass spectra without the assistance of a database.

De-novo protein sequence analysis

determination of amino acid composition

Amino acids are the building blocks of proteins, and are the fundamental nutrients for human beings. There are 20 amino acids for the composition of proteins. 10 out these amino acids can be synthesized by humans, and are known to be nonessential amino acids. Whereas, the rest cannot be produced by humans, and must be supplied in the food, hence, are known as essential amino acids. Composition and structure of these 20 amino acids are different, so as the their perspective functions.

Determination of amino acid composition

Peptide Mass Fingerprinting (PMF) is an analytical technique for identifying unknown protein. Proteins to be identified is first digested into peptides, whose absolute mass can be accurately analyzed by a mass spectrometer, such as MALDI-TOF or ESI-TOF. MALDI is more preferred in PMF analysis, as it has a wide sequence coverage and can analyze a large amount of sample at the same time. The peptide mass are compared to database containing the theoretical peptide masses of all known protein sequences. The match with the highest score was considered to be the identity of the unknown protein. 

Peptide mass fingerprinting

Creative Bioarray offers 35 human cell systems with over 160 different cell types. Moreover, we also provide our customers primary cells from over 13 types of other animals. These cells taken from living tissue are extremely accurate as they are literally coming from the source and are available from many sources on the human body. These living samples can give extremely accurate information about the cells in vivo and give relevant information regarding the living systems. more details via: http://www.creative-bioarray.com/Products/Primary-Cells.htm

Human Primary Cells

Creative Bioarray provides various human and animal cell lines that are invaluable for medical, scientific and pharmaceutical institutions. Creative Bioarray offers Rat Primary Cells for your research.

Check more: http://www.creative-bioarray.com/Products/Rat-Primary-Cells-list-175.htm

Bioarrayy

Home

Videos

Playlists

About

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.