Thermo Fisher Scientific Cell Biology

Pre-clearing is necessary to get good results? In fact pre-clearing is not necessary with Invitrogen Dynabeads Magnetic Beads. Is it really necessary to pre-clear your sample prior to immunoprecipitation? Let's say you have made the assumption that some proteins will always bind nonspecifically to your solid phase. In order to remove these proteins, you need to pre-clear the sample, right? Watch the video and learn how you can avoid background without any pre-clearing.

Myths about IP - Pre-clearing is necessary to get good results

In fact, you can save money using Dynabeads Magnetic Beads. 
Some people think that Dynabeads magnetic beads are expensive, while slurry is typically considered to be cheap. Yet the price per sample is comparable—or even lower—when you include the pre-clearing step. When you calculate the cost of doing your immunoprecipitation, you typically look at the cost of the solid phase relative to the potential capacity of a fixed volume of that solid phase. Watch the video to better understand how to calculate the actual cost of your immunoprecipitation experiments.

Myths of IP - Invitrogen Dynabeads magnetic beads are expensive

In fact High capacity is not always a good thing. When we say capacity is crucial, we are typically talking about the antigen-binding capacity. Because of the history of protein isolation and the move from large-scale protein purification to immunoprecipitation, people tend to believe that this capacity actually is crucial—when in fact capacity isn't always a good thing. The fact is that you want to balance the capacity, yield, specificity, and cost. Watch the video to learn why.

The myths about IP -  Higher capacity is better for IP

Background can't be avoided? With Dynabeads magnetic beads almost all background is removed.
It seems to be an established fact that background can't be avoided. Or is this a myth that now ought to be busted? Watch the video and learn how background can be avoided with Dynabeads magnetic beads.


Myths about IP - Background can't be avoided

Simple yet useful tips on how to design good primers for PCR reactions and what to avoid!

Thermo Fisher Scientific Cell Biology

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