Transfection

0:29 in vivo nucleic acid delivery: 
1:10 Why?
2:35 How?
4:40 What?
5:52 in vivo jetPEI®: easy, injectable, safe
9:40 in vivo delivery case studies:
9:50 AnxA2 function study
11:37 HIV-infected CNS attenuation
12:50 in vivo for gene therapy
13:15 in vivo jetPEI® Product Range: from R&D to Clinical Trials
15:42 Toolbox: transfection database, scientific materials
16:43 CONTACT US!

Check out our new webinar made by in vivo delivey experts on "in vivo delivery from study to gene therapy: overview, considerations and challenges" and learn how to succesully deliver nucleic acids in vivo.
Several topics are covered like the methods to generate an animal model, key parameters to consider beforehand and how to address in vivo delivery challenges.

[Webinar] in vivo delivery from study to gene therapy

Join this webinar hosted by BioInsight and featured by experts in transfection. Several topics are covered around the optimization of manufacturing scale-up of therapeutic viral vectors to support advanced clinical trials and commercialization.

Optimizing process development to simplify manufacturing scale up of therapeutic viral vectors

Tutorial provided to you by Transfection Experts. Transfection is used to study gene expression and protein function by introducing foreign genetic material into a cell. In this video we explain what transfection is. Why scientists use it. And the different way to transfect cells. As Polyplus-transfection is specialised in chemical-based transfection reagent, this part is emphasized. Thank you for listening and come and visit us at www.polyplus-transfection.com

What is transfection?

When using CRISPR/Cas9 system, transfection is a key and often limiting step to ensure successful genome editing. There are three main systems to transfect guide RNA and express Cas9 protein which are DNA, RNA or RNP (gRNA + Cas9 protein) approaches. Each system has their own pros and cons in terms of easiness of use, genome editing efficiency and off-targeting effects. Learn more about it on our website: https://www.polyplus-transfection.com/case-studies/genome-editing/

Webinar CRISPR: overview, challenges and latest technologies

jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:
- no risk of genome integration, hence no genome modification of the transfected cell
- no promoter regulation issue
- no need to reach the nucleus for efficient expression
more gentle process
jetMESSENGER® has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard to transfect, finally allowing relevant gene expression experiments in almost all cell types.

For more information, visit: https://www.polyplus-transfection.com/products/jetmessenger/

mRNA transfection tutorial using jetMESSENGER transfection reagent

FectoCHO® Expression System is a 3 component kit optimized to work together:
- FectoCHO® CD Expression Medium, a chemically defined cell culture medium optimized for transient gene expression
- FectoPRO® Transfection Reagent to produce amazing protein yields using low DNA amount
- FectoPRO® Expression Booster that enhances gene expression to boost the protein production

For more information, visit: https://www.polyplus-transfection.com/products/fectocho/

Transfection

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