Essen Bioscience

Considerable heterogeneity exists in even the simplest of cell systems. Watch this video to learn why cell-by-cell analysis is important. 

Why is cell-by-cell analysis important?

How non-invasive, long-term live cell analysis allows researchers to better understand the time line of biological processes and make informed decisions regarding their cell analysis workflows. Continuous cell monitoring also allows researchers to look at the morphological history of cells and measure holistic, integrated cell functions by following cell cultures over time and remotely.
Learn more at IncuCyte.com

The new age of live-cell analysis with the IncuCyte® System

Time-lapse movies acquired using IncuCyte® ZOOM live-cell analysis system showing real-time analysis of HT-1080 tumor cell proliferation following treatment with either the anti-cancer drug camptothecin (test) or vehicle (control). Proliferation is monitored by analyzing the cell occupied area (% confluence) of images over time. As cells die or proliferate, the cell confluence value either decreases or increases. IncuCyte ZOOM integrated image analysis tools enable label-free detection and measurement of confluence; an excellent surrogate for proliferation until the point that cells are densely packed or when large changes in morphology occur.

Assay concept:

● Measure cell proliferation using live cell time-lapse imaging, with or without labels. Easily generate long-term growth and growth-inhibition curves and monitor morphology
● Calculate growth rates and doubling times and explore co-cultures
● Multiplex proliferation readouts with measurements of apoptosis or cytotoxicity by combining with the IncuCyte™ Caspase 3/7 Reagent or IncuCyte™ Cytotox Reagents

Learn more at www.essenbio.com/proliferation

Tumor Cell Proliferation | Automatically Measure the Time Course with the IncuCyte® System

Quantitative live-cell imaging assays for immunotherapy: chemotaxis, immune cell killing &
phagocytosis

G. Lovell, N. Bevan, C. Szybut, N. Rodinova, D. Appledorn, M. Tikhonenko, L. O’Clair, T. O’Callaghan, T. Dale & D. Trezise.
Essen BioScience R&D, Welwyn Garden City, UK & Ann Arbor, Michigan, USA

Presented at CIMT 2016, Germany


Summary & impact:

• For the body to defend and fight against cancer, immune cells must recognise, engage, destroy and ultimately remove unwanted tumour cells. Understanding

these processes and interactions at the cellular level is central to identifying and validating new drug targets and cellular therapy approaches.

• Essen BioScience offers a flexible range of phenotypic assays to explore all aspects of the immuno-oncology research area. All of these assays are based on noninvasive live-cell analysis of cells in 96-well micro-plates using an IncuCyte® ZOOM live cell analysis system.

• Here we describe a cluster of new assays for quantifying immune cell biology and interactions with tumour cells.

• Phase-contrast/fluorescence images from cells gathered over time are processed to measure phenotypes, such as immune cell proliferation, migration & death.

• Experiments are performed with low cell numbers using simple mix and read formats which are non-perturbing to the cell model.

• Each of these approaches provide a full time-course of the biology; images and time-lapse movies provide credibility and valuable biological insight. Added throughput and automated image analysis enhances productivity.

IncuCyte Poster Snapshots | Quantitative Live-Cell Imaging Assays for Immunotherapy 

Time-lapse movies acquired using IncuCyte® ZOOM live-cell analysis system showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. Labeling of the SK-OV-3 cancer cells with the NucLight Red™ Live-Cell Labeling reagent enables direct counting of viable tumor cell numbers over-time. Addition of the IncuCyte™ Caspase 3/7 apoptosis reagent enables simultaneous detection of cells undergoing apoptosis (green fluorescence).

Assay concept:

● Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs).
● Tumor cell death is measured directly and in real-time by adding the mix-and-read IncuCyte™ Caspase 3/7 Reagent to your cultures.
● Tumor cell counts can be enabled by labeling target cells with NucLight™ Live-Cell Labeling Reagents
● Automated image analysis enables selective quantitation of tumor cell death.

Learn more at www.essenbio.com/immune-cell-killing

Immune Cell Killing of Tumor Cells | Automatically Measure the Time Course with the IncuCyte® System

Time-lapse movies acquired using IncuCyte® ZOOM live-cell analysis system showing real-time analysis of T cell clustering within a population of peripheral blood mononuclear cells (PBMC) following treatment with either anti CD-3 and IL-2 (activated) or vehicle (non-activated). Cell clustering, a measure of T cell activation, is automated monitored in real-time by counting the number of cell clusters within the image which have an area greater than a predefined area threshold. As the T cells are activated they begin to cluster and the number of counted objects increase over time. IncuCyte ZOOM integrated image analysis tools enable label-free detection of immune cell clustering and proliferation as a measure of immune cell activation.

Assay concept:

● Monitor immune cell proliferation and cell-cell clustering interactions in real time, without removing your cultures from the incubator
● Automated imaging and analysis – count label-free clusters using intuitive IncuCyte™ image analysis software
● Gain phenotypic insight from images and movies

Learn more at www.essenbio.com/immune-cell-activation

Essen Bioscience

Home

Videos

Playlists

About

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.