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3D Cell Explorer microscope from Nanolive, Switzerland.

The 3D Cell Explorer is a high speed, high resolution and non-invasive live cell imaging microscope that can look deep inside biological systems. This allows you to record stunning 3D images of entire cells in just seconds and with a higher resolution than any conventional microscope on the market.

 

For more information, visit nanolive.ch.

Live cell video of human mesenchymal stem cell undergoing mitosis

Live imaging of mouse pre-adipocytes for 48 hours. 1 image per minute. This is label-free yet you can see amazing biology unfolding under your eyes! This is unique material as never seen before.
Technology: 3D Cell Explorer microscope from Nanolive, Switzerland.

For more information, visit nanolive.ch.

Labelfree microscopy: Long Term Live Imaging of Mouse Pre-adipocytes (48hrs)



Blebbing, while fun to say, is also a defining feature of apoptosis (programmed cell death).  A bleb is more or less what it sounds like, a blob-like protrusion of the plasma membrane.  They generally exist for only a short time, on the scale of tens of seconds to a couple minutes.  The phenomenon is caused during apoptosis as the cell’s cortex, which plays a crucial role in maintaining cell shape, ruptures, or detaches from the plasma membrane, allowing it to bulge outwards.  From there, the bulges can either separate from the cell, becoming apoptotic bodies, or be reabsorbed by recontracting the bleb back into the cell.

Melanoma cells (Me 260.LN) blebbing

We’ve made a time lapse observation of mouse Embryonic stem cells (mESCs) using holo-tomographic microscope. <br/>Read more http://nanolive.ch/4-mitosis/

4 mESCs Mitosis synced up

Using glioblastoma cells, autophagy was induced by the addition of a drug.  The cells were incubated at 37 degrees with 5% CO2 and imaged every 60s over a period of 9 hours.
The cells used are LN18, and the drugs used are a combination of imipramine and ticlopidine. Special thanks to the Laboratory of Translational Oncology - Prof. Douglas Hanahan
Read more here: nanolive.ch/autophagy/

Glioblastoma cells undergoing autophagy

Bacteria lysis caused by T4 phage. The bacteria were imaged using the 3D Cell Explorer at 37°C for 2hrs with images being captured every 30 sec.

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