We are a dynamic, personal business with over 25 years’ experience supporting your essential needs for life science research. 

We do this with basic and advanced software for visualising and quantifying the separation of proteins, DNA and RNA by 1D SDS PAGE and 2D gel electrophoresis.

Our purpose? Easy-to-use, high-quality image analysis software to help you get results from core research techniques. We support over 2,600 software licenses in private and public organisations around the world. You can talk directly with one of our customers on every continent to hear what they think about us and our software. 

For more information, please visit » http://totallab.com

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In this video, I'll show you how you can obtain quantifiable data from lateral flow tests (LFT/LFA) in a 21 CFR Part 11/GxP/EU Annex 11 compliant manner using Phoretix 1D, which allows you to produce a full audit trail for every single lateral flow test you analyse to ensure regulatory compliance.

00:00 - Intro
00:20 - Explanation of TotalLab GxP Compliance Module
00:50 - How to log in to the software
01:40 - Username entry
01:50 - Password entry
02:17 - 21 CFR Sign in
02:23 - Project screen overview
02:32 - Creating a new compliant project
02:46 - Attaching notes to the audit trail of a project
03:10 - Adding files to your project
03:42 - Audit trail explanation
04:20 - Checking out a project for analysis
04:32 - Software integrations with the TotalLab GxP module
04:39 - Opening Phoretix 1D analysis software
04:50 - Analysing lateral flow tests
10:27 - Saving your analysis
10:32 - Audit trail build-up explanation
10:48 - Extended notes within the audit trail
11:40 - Checking a project back in to the secure folder
12:05 - Adding notes to a project check-in
12:23 - Checking the revision number of a project
12:54 - Viewing previous revisions of a project
13:08 - Viewing changes in data between revisions
13:41 - Exporting a 21 CFR compliant project report in .pdf
14:00 - Overview of report
15:20 - Sending a project for sign-off
15:50 - Adding notes for project sign-off to the audit trail
17:22 - Signing off a project
17:36 - Identifying projects awaiting sign-off
17:50 - Audit trail for project awaiting sign-off
18:36- Checking out a read-only copy of a project for inspection
19:20 - Password-protected sign-off
19:35 - Adding notes to project sign-off
21:05 - Project signed-off and closed

Quantifying the bands allows accurate comparison of the reactivity of different antibodies to positive samples and the definition of the limits of detection of those antibodies to positive samples (to determine the minimum testing sample volume).

A previous version of our 1D analysis software, CLIQS, has already been used for this purpose with the research published here: https://www.mdpi.com/2079-6374/11/12/510/htm

Another of our products, CLIQS 1D Pro, allows the building of a database of lateral flow tests that can be searched or allow tests to be compared to the entire database for similarities and differences.

For a free trial of Phoretix 1D to see how well it works with your lateral flow test images, please fill in the form here: https://totallab.com/products/phoretix-1d-free-trial/

For a free trial of CLIQS 1D Pro to see how well it works creating a database of your lateral flow test images, please fill in the form here: https://totallab.com/products/cliqs-1d-pro-free-trial/

For a free trial of our Phoretix 1D GxP software, to see how well it operates within a 21 CFR Part 11/GxP/Annex 11 compliant format, please fill in the form here:
https://totallab.com/products/gxp-module-demo/

#21CFR #GxP #lateralflowtest #LFA #analysis #GxP #database #software #LFT  #1D #imageanalysis #automatic #howto #antibody #testing

How to Analyse Lateral Flow Tests (LFT/LFA) in compliance with 21 CFR Part 11 Using Phoretix 1D

In this video, I'll show you how you can get free access to advanced Western blot/1D image quantification software.

00:00 - Intro
00:25 - Full free trial
00:40 - Free academic use licensing
01:10 - How to access free licensing mode
01:30 - Opening tutorial images
01:47 - Single-channel image analysis
03:03 - Image setup and contrast edits
03:45 - Automatic lane detection/manual lane detection
04:00 - Background removal
04:34 - Molecular weight marker import
05:10 - Multichannel image analysis

For a free trial of Phoretix 1D to see how well it works with your images, please fill in the form here:
https://totallab.com/products/phoretix-1d-free-trial/

Phoretix 1D can provide quantitative analysis of blot and gel images, lateral flow test images, TLC (thin layer chromotography) images and more.
 
See our other image analysis-based products here: https://totallab.com/products/

And if you want to get in touch with us here at TotalLab:

Website: http://www.totallab.com/ 
LinkedIn: https://www.linkedin.com/company/totallab-ltd/

#westernblot #electrophoresis #SDS-PAGE #tlc #quantification #immunoblot #software #imageanalysis #cliqs #totallab1d #imagej #21cfr #FDA #science #lifescience

Free 1D Gel or Blot Quantification Software Phoretix 1D - LINK IN DESCRIPTION

Calibration is an important part of some user's workflows, allowing harmonisation across your labs or even within the same lab using different imaging devices such as gel docs or densitometers to scan your immunoblots or electrophoresis gels. 

Our TotalLab GxP software fully supports calibration of images to convert pixel intensity to optical density or whatever other calibrated metric you wish to use. 

00:00 - Intro
01:35 - Why calibrating images is important
01:55 - How to import your stepwedge
02:45 - How to open the calibration mode
03:08 - False colour heatmap
04:08 - Creating a calibration map
05:20 - Quick sampling stepwedge to define steps
05:55 - Manual sampling points for calibration
06:30 - Standard curve/line of best fit for calibration data
08:40 - Equations for calibration data fitting
11:20 - Saving calibration files
13:20 - Applying calibration file to an image

Calibration is compatible with 1D or 2D images and our Phoretix or SameSpots/SpotMap image analysis products.
 
See our other image analysis-based products here: https://totallab.com/products/ where you can request a free trial of our software.

And if you want to get in touch with us here at TotalLab:

Website: http://www.totallab.com/ 
LinkedIn: https://www.linkedin.com/company/totallab-ltd/

#calibration #imagecalibration #westernblot #electrophoresis #SDS-PAGE #tlc #quantification #immunoblot #software #imageanalysis #totallab1d #imagej #21cfr #FDA #lifescience

How to Calibrate 1D or 2D Gels or Blots Using a Stepwedge in Phoretix 1D


http://totallab.com/spot-map » Accurate quantitation is an important part of your HCP analysis. In this video we show you how automatic spot outlines in SpotMap obtain an objective, accurate and quantitative analysis and how you can use 3D mode to validate the software you currently use.

Are you ready to try SpotMap free for 14 days? Just fill out the SpotMap demo form on our website » http://totallab.com


SpotMap: Accurate Quantitative Analysis for HCP Coverage 

Good image capture is critical to guarantee optimal performance of automated image analysis packages and generate reliable scientific data. This checklist defines some of the important technical factors required to generate digital images of a quality suitable for automated image analysis. Download our image capture guide here: http://totallab.com/cms/pdf/imagecaptureebook.pdf

Image Quality Hints and Tips- Checklist 

TotalLab software will analyse colour images. If the software loads a colour image it is automatically converted to grayscale for analysis. However, we recommend using greyscale images as you have the potential to get more data from your analysis. A colour image is made up of 3 different channels; Red, Green and Blue. So a 24-bit colour image has 8-bits per channel. When the image is converted to grayscale, it’s effectively changed to an 8-bit grayscale image with weighted conversion of the Red, Green and Blue channels. So a colour image, when imported to the software, will lose information and be converted in a way that may not be accurate for the image capture device used. Always scan directly to grayscale as the imaging device will then do the conversion from colour in the most accurate and sensitive manner. For more information download our image capture guide: http://totallab.com/cms/pdf/imagecaptureebook.pdf

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