BMG LABTECH

The CLARIOstar microplate reader is used to run a variety of assays mainly focussing on cell signalling and protein-protein interaction studies, adopting fluorescence complementation and luminescence complementation techniques.

More information about the product: https://www.bmglabtech.com/clariostar/

Understanding the basic mechanisms and physiology of GPCRs

Sygnature Discovery is a Contract Research Organisation (CRO) specialized in supporting clients´ projects by providing integrated drug discovery resources. This is mainly achieved by assessing the pharmacology and functionality of novel compounds or targets.


Sygnature Discovery routinely utilises a plethora of biochemical assays including BRET, TR-FRET, HTRF, absorbance, fluorescence intensity and polarization and AlphaLISA in different microplate formats all of which are measured on the PHERAstar FSX. The microplate reader was chosen for its sensitivity, reliability and flexibility. The flexibility of the reader helps to match the diverse and changing needs CROs have to face when tackling different drug discovery projects. The sensitivity and consistent reliability enabled the design of very challenging bespoke assays that could to be performed on other assays.

More Information: https://www.bmglabtech.com/pherastar-fsx/

PHERAstar FSX supports the diverse and changing needs of CROs

Laura Kilpatrick, Chloe Peach and Carl White from the University of Nottingham talk about Cancer Research and how they use our Microplate Readers PHERAstar and CLARIOstar.

Analysing the pathophysiological role played by VEGFR2 and CXCR4

Maria Arruda is part of the cell signaling group of the University of Nottingham and describes how she uses the CLARIOstar for her research.

Drug discovery using natural products from the Brazilian biodiversity on the CLARIOstar

Prof. Dmitry B. Veprintsev and Prof. Dr. David Sykes from the Molecular and Cellular Pharmacology of the University of Nottingham, show how you can do GPCR binding and signalling studies with the PHERAstar.

GPCR Binding and signalling studies with the PHERAstar

In our latest webinar, speaker Dr. Gene Ananiev from the Small Molecule Screening Facility at the University of Wisconsin Madison, discusses two examples of high-throughput screening in 1536-well plates, presenting methodology for assay miniaturisation and optimisation.

The two projects were set up for customers and include a cell-based luciferase reporter assay and an AlphaScreen-based screen for protein-protein interactions.

In the first project, CRISPR/Cas9 was used to knock in a Nano luciferase (Nluc) gene into the endogenous human FMR1 gene in neural progenitor cells. This was done in order to create a robust FMR1-Nluc reporter hiPS cell line. Functional assays confirmed that the Nluc activities accurately report FMR1 gene expression levels. Moreover, neural progenitor cells derived from this cell line were optimised for high-throughput screening.

In the second project, the focus was on antibiotic-resistant bacterial infections. As antibiotic resistance is on the rise worldwide, there is an urgent need to identify novel classes of antibiotics that can overcome resistance mechanisms. In this study, nine efficient inhibitors have been identified. They produced response curves with IC50 values of <40 μM. Additionally, two compounds directly bound to PriA, demonstrating the success of this screening strategy. Using a miniaturised AlphaScreen test, costs were drastically reduced and throughput for protein-protein interaction was increased.

In addition to Dr. Ananiev, Dr. Carl Peters from BMG LABTECH and Dr. Iain Russell from Labcyte highlight the microplate reader and dispensing solution that have been used in the projects.

BMG LABTECH

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