Lonza

In this online symposium Drs. Jessica Hartman and Martin Phillips present 2D and 3D models being developed by ScitoVation for assessing hepatotoxicity. By combining Lonza’s primary hepatocytes and liver non-parenchymal cells in 2D or 3D models, the viability, phenotypic behavior, and response of cells to chemicals is enhanced.

This webcast explores how scientists can improve the physiological relevance of their in vitro liver models, while ensuring the complexity of cultures is kept to a minimum, saving laboratories both time and money.

Topics covered include:

- Hepatocytes as a metabolically active, in vitro test system
- Comparison of existing technologies
- Alginate encapsulation for testing slowly-cleared chemicals
- Development of 2D and 3D organotypic rat liver models

Designed for Purpose - Complex Liver Cell Cultures for Improving In Vitro Hepatotoxicity Testing

Lonza’s Nucleofector™ Technology is an improved electroporation technology that can help you achieve high transfection efficiency in primary cells, stem cells and hard to transfect cell lines. This video shows how to use the X Unit of the 4D-Nucleofector™ System with its two different vessel types: The 100 microliter cuvettes for high cell numbers at low throughput (e.g. for biochemical applications or Western Blots) and the 20 microliter 16-well strips for low cell numbers at medium throughput (e.g. reporter gene assays, RNAi, CRISPR). Nucleofection conditions are transferable between both vessel types offering maximum flexibility and convenience. Learn more about the 4D-Nucleofector™ System at <a href="http://www.lonza.com/4d" target="_blank">www.lonza.com/4d</a>

4D-Nucleofector™ X Unit – For Flexible Non-Viral Transfection of Hard-to-Transfect Cells

The handling and care of cryopreserved hepatocytes is crucial to achieving consistent cell yield and viability from any lot. This video guide provides step-by-step instructions on the proper way to thaw, plate, and incubate cryopreserved hepatocytes for optimal results.

Thaw, Plate, Incubate: Cryopreserved Hepatocyte Thawing and Plating Instructions

This video shows the technology behind the RAFT[trade/] 3D Cell Culture System and how it creates high-density collagen scaffolds with cells embedded. The RAFT[trade/] System has successfully been used in many research areas including toxicology, oncology, blood brain barrier models, corneal models and more. Coming along with easy-to-follow protocols, RAFT[trade/] System allows you to set up robust 3D cultures in less than an hour. Learn more about RAFT[trade/] System on www.lonza.com/raft-3d-culture.com.

Simplify Your 3D Cell Culture with a Novel Method for Tissue Modeling

Not all researchers are maintaining correct supervision of their cells while being cultured in the incubator. This led to a worldwide trend of shocking cell misbehavior generating meaningless results. Do YOU know what your cells get up to when you are not watching them?  Lonza News with Leigh Eastwood show what is really happening in your incubator and how suspicious cell conduct can be avoided!

Lonza

Home

Videos

Playlists

About

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.