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Novus Biologicals Visual Protocols: In phase 2 of this video you will learn how to effectively shear chromatin for ChIP using a sonicator, and how to analyze DNA fragment sizes with an agarose gel. Components of the Novus ChromataChIP kit, an all in one ChIP solution, are also shown. Full length protocols and additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

Chromatin Immunoprecipitation: Phase 2

Novus Biologicals Visual Protocols: In this video you will learn how to add your secondary antibody, wash the plated cells, double label, and mount your coverslips onto slides for analysis. Full length protocols and additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

Chromatin Immunoprecipitation: Phase 3

Novus Biologicals Visual Protocols: In phase 4 of this video you will learn how to reverse the DNA-protein cross-linking, followed by DNA purification through silica based cleanup columns. Your precipitated and cleaned DNA can then be analyzed by a variety of detection methods discussed in the final phase. Components of the Novus ChromataChIP kit, an all in one ChIP solution, are also shown. Full length protocols and additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

Chromatin Immunoprecipitation: Phase 4

Novus Biologicals Visual Protocols: In phase 5 of this video you will learn how load your precipitated and cleaned DNA into a microwell plate for amplification and detection through quantitative real-time PCR. Components of the Novus ChromataChIP kit, an all in one ChIP solution, are also shown. Full length protocols and additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

Chromatin Immunoprecipitation: Phase 5

Novus Biologicals Visual Protocols: In this video you will learn how to clean and coat coverslips, prepare cell culture plates, and plate cells for ICC. Full length protocols and additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

Immunocytochemistry: Phase 1

Novus Biologicals Visual Protocols: In this video you will learn how to fix, permeabilize, block, and add primary antibody to your plated cells. Full length protocols and additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

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