SiliCycle Inc.

SiliCycle Scavenging Solutions

Get a quick overview on how a sample is analyzed using High Performance Liquid Chromatography (HPLC).

As the sample mixture in the mobile phase is pumped into the column, the analytes or compounds in it interact with the stationary phase (ie sorbent). The overall interactions make it that the compounds get retained with varying strength, thereby affecting when they exit the column. The detector at the exit records this elution and the information generates the chromatogram. 

Have more questions regarding HPLC? SiliCycle has compiled a list of the most frequently asked questions here: https://www.silicycle.com/faq/hplc 

If new to HPLC, it is also important to note that keeping the column in top condition with proper care will help to maintain column efficiency over an extended period of time, so you can continue your testing for as long as possible. This Application Note shares different procedures used to help extend the lifetime of HPLC columns. https://www.silicycle.com/resource-center/application-notes/hplc-columns-cleaning-regeneration-procedures

HPLC Analysis in a nutshell - How a sample is analyzed 

E-PAK is a family of radial flow purification cartridges that linearly scale-up from lab to commercial processing sizes, which means that lab conditions and results are easily reproducible. 

They are four ways/ configurations that one can use E-PAK cartridges:
-Single Pass
-Recirculation
-Multi pass
-Series pass

Watch the video to see each one in action. 

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Also be sure to check out our Application Notes library to see industry examples of when and how to use E-PAK:  
https://www.silicycle.com/resource-center/application-notes


Methods of using E-PAK

E-PAK is a family of radial flow purification cartridges developed specifically for pharmaceutical processing from the lab scale all the way up to commercial sizes. 

Scaling up is easy when  it comes to E-PAK as it follows a linear trend, that way you can be sure that your lab results can be easily achieved at pilot and commercial purification steps.    

Offered with a choice of metal scavengers, bare silica or activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Have questions about metal scavengers? Visit our FAQ page: https://www.silicycle.com/faq/metal-organic-scavengers

SiliCycle also has a product blog page and frequently touches on the drug purification. Some articles that may be of interest include;
Excelling at scaling-up: https://www.silicycle.com/articles/cat/product-blog/post/some-people-excel-at-scaling-up-their-purifications-and-some-dont-which-one-are-you
Streamlining scale-up: https://www.silicycle.com/articles/cat/product-blog/post/how-to-streamline-metal-scavenger-scale-up-during-drug-development/
Simplifying catalyst removal: https://www.silicycle.com/articles/cat/product-blog/post/simplifying-metal-catalyst-removal-in-drug-development-applications/
Eliminating metal contamination: https://www.silicycle.com/articles/cat/product-blog/post/pharmaceutical-metal-scavengers/


E-PAK cartridge housings, from lab to commercial scale

Created with proprietary technology, E-PAK radial flow cartridges provide rapid adsorption kinetics and processing capacities for laboratory, pilot and commercial operations. They are designed for use with both organic and aqueous solvents, and incorporate design features useful for the production of active pharmaceutical ingredients (API).

Features include:
• Proven cartridge design to ensure rapid, simple & reliable technology
• Fixed-bed design ensuring safer handling, clean-up and disposal
• Large adsorbent capacity in small area footprint increases product recovery & reduces solvent requirements

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Take a closer look at the E-PAK cartridge

E-PAK is a family of radial flow purification cartridges developed specifically for pharmaceutical processing.

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

Have questions about metal scavengers? Visit our FAQ page: https://www.silicycle.com/faq/metal-organic-scavengers

Also check out our Application Notes library to see some examples of when  and how to use E-PAK cartridges to help with purification needs:   https://www.silicycle.com/resource-center/application-notes


What are E-PAK radial flow cartridges?

Flash chromatography is a widely established chemical separation technique based on the polarity differences of compounds. General the following will influence the efficiency and success of your separation and purification:

1-Methodology
2-Theory of separation
3-Choice of solvent
4-Loading the column
5-Solvent equilibration
6-Loading of the sample
7-Column capacity

To know more about the principal common steps listed above and some rules of thumb for performing flash chromatography read this Application Note:  https://www.silicycle.com/resource-center/application-notes/quick-overview-of-column-chromatography

Have more questions about Flash Chromatography or about the sorbents you can use? Visit our FAQ page : https://www.silicycle.com/faq

Prior to performing a separation with a chromatographic column, it is highly recommended to perform screening tests using thin layer chromatography (TLC) to determine the best conditions for the separation. To understand the relationship and how one transfers to the other, our FAQ video series is great. Videos available on LabTube.

SiliCycle Inc.

Home

Videos

Playlists

About

Keep up to date with all your favourite videos and channels.

Get personalised notifications on new releases and channel content by subscribing to the LabTube eNewsletter.