SiliCycle Inc.

SiliCycle Scavenging Solutions

The relationship between retention factors and column volumes is important to transfer from TLC to flash chromatography.

Have more questions? Head over to our FAQs:
https://www.silicycle.com/faq

Learn more about SiliaSep:
https://www.silicycle.com/products/siliasep-flash-cartridges

Learn more about SiliaPlate:
https://www.silicycle.com/products/thin-layer-chromatography-tlc-plates

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FAQ What is the relationship between retention factor and column volume?

The basis of smoothly going from TLC plates to flash chromatography starts with the retention factor (Rf).  

Inaccurate Rf’s will lead to erroneous column volumes (CVs) and then completely skew all the method development. 

Have more questions? Visit our TLC FAQ guide: https://www.silicycle.com/faq/thin-layer-chromatography

Get TLC best practices with our blog article:
https://www.silicycle.com/articles/cat/product-blog/post/thin-layer-chromatography-plates-use-and-best-practices/

Learn more about SiliaPlate:
https://www.silicycle.com/products/th...


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FAQ How to calculate retention factor (Rf)?

Don't be fooled,  column volume does not mean the internal volume of  the column without the packing material inside!
Also known as CV, column volumes are an important part of column chromatography as they help determine purification run length, gradient profile, and solvent consumption.  

Get an more in-depth overview of column chromatography with our free Application Note:
https://www.silicycle.com/resource-center/application-notes/quick-overview-of-column-chromatography

Have more questions? Visit our FAQ guide: 
https://www.silicycle.com/ca/faq

Learn more about SiliaSep Flash Cartridges: https://www.silicycle.com/products/siliasep-flash-cartridges


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Brighter Days by Markvard & Kvarmez 
https://soundcloud.com/markvard
https://soundcloud.com/simen-eliassen...
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FAQ Understanding Column Volume

One of the the questions most received at SiliCycle when researchers are looking to try functionalized silica gel for their purification applications, is :
What pH range is suitable when working with functionalized silica gel? 

This is often followed by : What happens to the gel outside this pH range?

Watch this Q&A video to find out the answer.

Have more questions regarding functionalized or even bare silica? Visit our FAQ guide : https://www.silicycle.com/faq/functionalized-silica

Interested in knowing what kind of bonded phases exist and their applications?
https://www.silicycle.com/products/complete-range-of-functionalized-silicas

Learn more about SiliaBond bulk gels:
https://www.silicycle.com/products/functionalized-silica-gels

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Brighter Days by Markvard & Kvarmez 
https://soundcloud.com/markvard
https://soundcloud.com/simen-eliassen...
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Free Download / Stream: https://bit.ly/brighter-days
Music promoted by Audio Library https://youtu.be/jVEoeR9AunY

FAQ What pH range is suitable when working with functionalized silica?

E-PAK is a family of radial flow purification cartridges developed specifically for pharmaceutical processing.

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges

SiliCycle Inc.

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