SiliCycle Inc.

SiliCycle Scavenging Solutions

This Q&A video will show you the two different ways to determine how much scavenger will be needed in your purification based on your solution parameters.

What are metal scavengers?
Metal Scavengers are functionalized silica gels designed to react and bind metal complexes. They are used to remove metallic impurities from your chemistry.

Metal scavengers are offered in various formats:

- as bulk (SiliaMetS functionnalized silica gels)
https://www.silicycle.com/products/metal-scavengers

- packed in "flash" cartridges (SiliaSep)
https://www.silicycle.com/products/siliasep-flash-cartridges

- packed in SPE cartridges (SiliaPrep)
https://www.silicycle.com/products/silicycle-sample-preparation-products/spe-cartridges

- packed in flow-through fixed bed cartridges (E-PAK)
https://www.silicycle.com/products/e-pak-purification-cartridges

FAQ How to calculate the right amount of scavenger to remove metal / organic impurities

Created with proprietary technology, E-PAK radial flow cartridges provide rapid adsorption kinetics and processing capacities for laboratory, pilot and commercial operations. They are designed for use with both organic and aqueous solvents, and incorporate design features useful for the production of active pharmaceutical ingredients (API).

Features include:
• Proven cartridge design to ensure rapid, simple & reliable technology
• Fixed-bed design ensuring safer handling, clean-up and disposal
• Large adsorbent capacity in small area footprint increases product recovery & reduces solvent requirements

Offered with a choice of metal scavengers, bare silica & activated carbons, these cartridges are designed to remove precious metal catalysts, reaction-by-products or color from synthetic reaction mixtures and natural products.

For more information on E-PAK, visit us at:
https://www.silicycle.com/products/e-pak-purification-cartridges


Music Credit: 
Brighter Days by Markvard & Kvarmez

E-PAK cartridge 3D animation  of API filtration

Watch a time lapse of  a dye mixture having each colour separated and isolated via flash chromatography using bare silica spherical gel.  

Procedure 
Cartridge : FLH-10095D-A-ISO40 (https://www.silicycle.com/products/siliasep-flash-cartridges/portfolio/siliasep-premium/flh-10095d-a)
Solvent : A = Methanol, B = Ethyl Acetate 
Conditioning : 100% Ethyl Acetate, 1 min at 100 mL/min 
Injection : 0.5 mL via liquid loading 
Gradient : 
-Segment 1 : 100%B, for 1 min at 100 mL/min 
-Segment 2 : 100-55% B, 5 min at 100 mL/min 
-Segment 3 : 55% B, 6 min at 100 mL/min 

 Our flash chromatography products offer high levels of performance and reproducibility. The flash media consistently has a tight particle size distribution with low fines and extremely low metal ion content, for more predictable and easier separations.

Using SiliCycle SiliaSep cartridges, you can purify your sample base on different mechanisms depending on the properties or your compound. Learn more about SiliaSep PREMIUM
https://www.silicycle.com/products/siliasep-flash-cartridges/portfolio/siliasep-premium

Flash Separation of Dye Mixture with 25 μm spherical bare silica gel (Time Lapse)

Particle size distribution is one of seven characteristics that is important to consider when selecting a silica product for your chromatographic applications. As such, it is good to understand exactly what it is and how it impacts column performance so that the best product for the separation or purification is chosen. 

Read more about  the seven key characteristics to consider here: https://www.silicycle.com/articles/cat/product-blog/post/use-of-silica-in-chromatography-understanding-key-physical-characteristics/

Next find out how to determine which chromatographic phase is best suited for your application: https://www.silicycle.com/articles/cat/product-blog/post/how-to-choose-the-right-chromatographic-phase/



Music Credit: 
Brighter Days by Markvard & Kvarmez 
https://soundcloud.com/markvard
https://soundcloud.com/simen-eliassen...
Creative Commons — Attribution 3.0 Unported  — CC BY 3.0 
Free Download / Stream: https://bit.ly/brighter-days
Music promoted by Audio Library https://youtu.be/jVEoeR9AunY

FAQ Understanding particle size distribution - D50, D90 and D10

The relationship between retention factors and column volumes is important to transfer from TLC to flash chromatography.

Have more questions? Head over to our FAQs:
https://www.silicycle.com/faq

Learn more about SiliaSep:
https://www.silicycle.com/products/siliasep-flash-cartridges

Learn more about SiliaPlate:
https://www.silicycle.com/products/thin-layer-chromatography-tlc-plates

Music Credit: 
Brighter Days by Markvard & Kvarmez 
https://soundcloud.com/markvard
https://soundcloud.com/simen-eliassen...
Creative Commons — Attribution 3.0 Unported  — CC BY 3.0 
Free Download / Stream: https://bit.ly/brighter-days
Music promoted by Audio Library https://youtu.be/jVEoeR9AunY

FAQ What is the relationship between retention factor and column volume?

The basis of smoothly going from TLC plates to flash chromatography starts with the retention factor (Rf).  

Inaccurate Rf’s will lead to erroneous column volumes (CVs) and then completely skew all the method development. 

Have more questions? Visit our TLC FAQ guide: https://www.silicycle.com/faq/thin-layer-chromatography

Get TLC best practices with our blog article:
https://www.silicycle.com/articles/cat/product-blog/post/thin-layer-chromatography-plates-use-and-best-practices/

Learn more about SiliaPlate:
https://www.silicycle.com/products/th...


Music Credit: 
Brighter Days by Markvard & Kvarmez 
https://soundcloud.com/markvard
https://soundcloud.com/simen-eliassen...
Creative Commons — Attribution 3.0 Unported  — CC BY 3.0 
Free Download / Stream: https://bit.ly/brighter-days
Music promoted by Audio Library https://youtu.be/jVEoeR9AunY

SiliCycle Inc.

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